breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 16,990 IS150 (+) +3 bp intergenic (‑32/‑496) mokC ← / → nhaA regulatory protein for HokC, overlaps CDS of hokC/pH‑dependent sodium/proton antiporter
RA 365,031 C→A V249V (GTC→GTA sbmA → predicted transporter
RA 474,884 A→C L141* (TTA→TGA)  ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
JC JC 1,305,487 IS150 (–) +3 bp coding (751‑753/1461 nt) cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,500,863 G→A A134T (GCG→ACG)  tktB → transketolase 2, thiamin‑binding
JC 2,511,675 Δ8 bp intergenic (+139/‑393) acrD → / → yffB aminoglycoside/multidrug efflux system/hypothetical protein
RA 3,447,556 T→C E64G (GAA→GGA)  aroK ← shikimate kinase I
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,928,901 G→T A229S (GCT→TCT)  rho → transcription termination factor Rho
RA 4,162,470 A→C T539P (ACC→CCC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,202,303 +TG coding (257/825 nt) iclR ← DNA‑binding transcriptional repressor
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 4275743 =41 (0.900)37 (0.940) 33/130 0.2 52.3% coding (205/690 nt) yjcO hypothetical protein
?REL606 = 4359351 32 (0.810)coding (1679/1809 nt) frdA fumarate reductase