Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A61 F1 I1 R1
|
19 |
50.7 |
3232285 |
96.8% |
3128851 |
74.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,625,089 |
G→T |
P156Q (CCG→CAG) |
rncS ← |
ribonuclease III |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,625,089 | 0 | G | T | 100.0%
| 138.0
/ NA
| 33 | P156Q (CCG→CAG) | rncS | ribonuclease III |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/19); total (14/19) |
CGGACCTGAACTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTTCTCG > REL606/2625017‑2625160
|
cGGACCTGAACTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGa < 2:798197/75‑1 (MQ=255)
tGAACTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCtttt > 2:894493/1‑75 (MQ=255)
cTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGtt < 1:674970/75‑1 (MQ=255)
cTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGtt > 2:1582155/1‑75 (MQ=255)
tACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGttt < 1:1086770/75‑1 (MQ=255)
tAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCt < 2:1379778/75‑1 (MQ=255)
tAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCt > 2:1321849/1‑75 (MQ=255)
aGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTgg < 2:751576/75‑1 (MQ=255)
gTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTggg < 1:732009/75‑1 (MQ=255)
tCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGc < 1:1216283/75‑1 (MQ=255)
gcggcagATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAAttt < 1:643102/75‑1 (MQ=255)
ggcagATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCg > 1:89785/1‑75 (MQ=255)
agATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTcc > 2:296517/1‑75 (MQ=255)
aTGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAg > 1:1348162/1‑75 (MQ=255)
acgacCCTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACg > 2:101097/1‑75 (MQ=255)
ccTGCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGttt < 1:1523515/75‑1 (MQ=255)
gCAAATATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGAt < 1:857176/75‑1 (MQ=255)
aaTATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATAcc > 1:458247/1‑75 (MQ=255)
aaTATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATAcc > 2:383150/1‑75 (MQ=255)
aaTATTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATAcc < 2:886928/75‑1 (MQ=255)
atatTCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCa > 2:1140694/1‑75 (MQ=255)
ttCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGtt < 1:1170705/75‑1 (MQ=255)
tCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTg < 2:1224040/75‑1 (MQ=255)
tCTTGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTg > 2:1592548/1‑75 (MQ=255)
tGCAAGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAgg < 1:750287/75‑1 (MQ=255)
aGCGCGTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTa > 1:239985/1‑75 (MQ=255)
cgcgTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAAt < 1:232938/75‑1 (MQ=255)
gcgTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGACTAAtt < 2:342752/75‑1 (MQ=255)
cgTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAAttt < 1:524987/75‑1 (MQ=255)
cgTTTTCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAAttt < 1:524983/75‑1 (MQ=255)
tttCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTtctc < 1:1158834/75‑1 (MQ=255)
tttCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTtctc > 2:1130985/1‑75 (MQ=255)
ttCTGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTTCTCg > 2:907588/1‑75 (MQ=255)
|
CGGACCTGAACTACCAGATAAGTCGGCAGCGGCAGATGACGACCCTGCAAATATTCTTGCAAGCGCGTTTTCGGATCTTTTTGTTTATCGCCTGGGCTAATTTCGTCCAGACGAGTTTGATACCAGTTGAGGATTAATTTCTCG > REL606/2625017‑2625160
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
