Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A61 F1 I1 R1
|
19 |
50.7 |
3232285 |
96.8% |
3128851 |
74.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,247,298 |
A→G |
D238D (GAT→GAC) |
truB ← |
tRNA pseudouridine synthase B |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,247,298 | 0 | A | G | 91.4%
| 204.4
/ 8.3
| 58 | D238D (GAT→GAC) | truB | tRNA pseudouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base G (31/22); total (34/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 |
CAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGT > REL606/3247228‑3247369
|
cAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCaa < 2:254554/75‑1 (MQ=255)
gaagtCGTTAACGGAAGATTCACCCCCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGt < 1:1190531/75‑1 (MQ=255)
aagaCGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTa < 2:695308/75‑1 (MQ=255)
aagaCGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTa < 2:631535/75‑1 (MQ=255)
aagaCGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTa < 2:230969/75‑1 (MQ=255)
gaCGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAAc < 2:582591/75‑1 (MQ=255)
cGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTc < 1:634568/75‑1 (MQ=255)
tAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCcgc > 1:1079553/1‑75 (MQ=255)
tAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCcgc < 2:590451/75‑1 (MQ=255)
gATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGaa < 2:1066653/75‑1 (MQ=255)
gATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGaa > 1:388237/1‑75 (MQ=255)
aTTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAat > 2:923088/1‑75 (MQ=255)
aTTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAat < 2:545315/75‑1 (MQ=255)
tCACCGCCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAatat < 2:115187/75‑1 (MQ=255)
caccacCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATc > 2:478479/1‑75 (MQ=255)
accaccGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATcc > 1:1350044/1‑75 (MQ=255)
ccaccGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCct < 2:516673/75‑1 (MQ=255)
caccGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCctg > 2:1285882/1‑75 (MQ=255)
caccGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTCCGCGGCTGGAACATcccg > 2:396746/1‑73 (MQ=255)
accGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTCCGCGGCTGGAATATCctgc < 1:3261/75‑1 (MQ=255)
ggATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATTGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGt > 1:679031/1‑75 (MQ=255)
ggATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGt > 1:1469817/1‑75 (MQ=255)
gATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGtt < 1:1418211/75‑1 (MQ=255)
tAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCa < 2:1378971/75‑1 (MQ=255)
tAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCa < 2:373530/75‑1 (MQ=255)
tAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCa > 1:739969/1‑75 (MQ=255)
aGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAg > 2:221993/1‑75 (MQ=255)
tCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCt > 2:1466258/1‑75 (MQ=255)
aGCTGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTc > 2:1244223/1‑75 (MQ=255)
tGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTcaac > 1:772107/1‑75 (MQ=255)
tGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTcaac > 1:1112390/1‑75 (MQ=255)
tGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTcaac > 2:1578446/1‑75 (MQ=255)
tGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTcaac < 1:408652/75‑1 (MQ=255)
tGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTcaac < 1:408637/75‑1 (MQ=255)
tGGACTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTcaac > 1:206029/1‑75 (MQ=255)
cTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGt < 2:1585565/75‑1 (MQ=255)
cTGTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGt > 1:766074/1‑75 (MQ=255)
gTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTc < 2:1586580/75‑1 (MQ=255)
gTCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTc < 1:32538/75‑1 (MQ=255)
tCCATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCa > 2:1391892/1‑75 (MQ=255)
ccATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCAc < 1:1511923/75‑1 (MQ=255)
ccATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCAc > 2:416591/1‑75 (MQ=255)
cATTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACg > 1:1590772/1‑75 (MQ=255)
aTTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGc < 2:109749/75‑1 (MQ=255)
aTTGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGc > 1:926592/1‑75 (MQ=255)
ttGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCa > 2:665289/1‑75 (MQ=255)
ttGGCATCAGTAATGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCa > 2:434581/1‑75 (MQ=255)
tGGCATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAg > 2:1123195/1‑75 (MQ=255)
cATCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTg > 1:1052921/1‑75 (MQ=255)
tCAGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCt > 2:1060669/1‑75 (MQ=255)
aGTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTcc > 1:1133810/1‑75 (MQ=255)
gTAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCa > 1:1523320/1‑75 (MQ=255)
tAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAg > 1:878604/1‑75 (MQ=255)
tAATGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAg > 1:1198574/1‑75 (MQ=255)
aaTGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAgg > 1:775640/1‑75 (MQ=255)
aTGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAggg > 1:1246130/1‑75 (MQ=255)
aTGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAggg < 1:1027142/75‑1 (MQ=255)
tGGGTCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGt > 1:1474935/1‑75 (MQ=255)
|
CAGAAGACGTTAACGGAAGATTCACCACCGGATAGTCCGAAGCTGGACTGTCCATTGGCATCAGTAATGGATCAAGTAACTCCGCGGCTGGAATATCCTGCTGTTCAGCTTGCTCAACAAGTTCACGCAGGTGCTCCAGGGT > REL606/3247228‑3247369
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
