Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A61 F1 I1 R1
|
19 |
50.7 |
3232285 |
96.8% |
3128851 |
74.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,497,971 |
G→A |
A22V (GCT→GTT) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,497,971 | 0 | G | A | 100.0%
| 130.9
/ NA
| 33 | A22V (GCT→GTT) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/21); total (12/21) |
AACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCG > REL606/3497899‑3498042
|
aaCGTTGCGATCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACg > 2:547417/1‑75 (MQ=255)
tGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATaga > 2:217385/1‑75 (MQ=255)
aTGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTc < 2:1419304/75‑1 (MQ=255)
tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCt < 1:757604/75‑1 (MQ=255)
tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCt < 2:1229491/75‑1 (MQ=255)
tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCt > 2:804397/1‑75 (MQ=255)
gTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTa > 2:1098344/1‑75 (MQ=255)
tttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTAc < 1:355253/75‑1 (MQ=255)
tttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTAc < 2:1548471/75‑1 (MQ=255)
tttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTAc < 1:141320/75‑1 (MQ=255)
acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTg < 1:217385/75‑1 (MQ=255)
gacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTggg < 1:30391/75‑1 (MQ=255)
gacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTggg < 1:913962/75‑1 (MQ=255)
acCGGGTCCTTTCCAATCGTAAACATCACCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGc > 1:563488/1‑75 (MQ=255)
gggTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGAt > 1:18231/1‑75 (MQ=255)
gggTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGAt < 2:6830/75‑1 (MQ=255)
ggTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATg < 1:810939/75‑1 (MQ=255)
tttCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAatat > 1:1269487/1‑75 (MQ=255)
tCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGt < 2:836207/75‑1 (MQ=255)
tCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGt < 2:795107/75‑1 (MQ=255)
tCCAAGCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGt < 1:1271292/75‑1 (MQ=255)
cAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTaa < 2:1563023/75‑1 (MQ=255)
cAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTaa < 1:479480/75‑1 (MQ=255)
aTCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAAc < 2:827417/75‑1 (MQ=255)
gTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGa > 2:540228/1‑75 (MQ=255)
aaaCATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGc > 2:785375/1‑75 (MQ=255)
tCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCa < 2:171825/75‑1 (MQ=255)
cAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCat > 1:476831/1‑75 (MQ=255)
aGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCata < 2:202745/75‑1 (MQ=255)
cTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCAtata > 2:1593093/1‑75 (MQ=255)
ttGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAg < 2:378081/75‑1 (MQ=255)
tGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAgg < 2:286904/75‑1 (MQ=255)
tAAACGATAGAGTGCTTAAGGGATTCTACGCTAAGCGAGGGCGATGAATATGTAAACGGAGCATTCATATAGGCg > 2:957517/1‑75 (MQ=255)
|
AACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCG > REL606/3497899‑3498042
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
