Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
19 |
34.1 |
2156969 |
96.4% |
2079318 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,497,971 |
G→A |
A22V (GCT→GTT) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,497,971 | 0 | G | A | 100.0%
| 141.3
/ NA
| 39 | A22V (GCT→GTT) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/20); total (19/20) |
TAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCG > REL606/3497898‑3498042
|
tcaCGTGGCGTTCAGCCATTCANGTGTATTGGCNACGACCGGNTCCTTTCCAATCGTAAACATCANCTTGTAAACg < 2:942501/74‑1 (MQ=255)
tAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTACCAATCGTAAACATCAGCTTGTAAACg < 1:98886/76‑1 (MQ=255)
gTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATag < 1:497812/76‑1 (MQ=255)
ttGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATaga < 2:119256/76‑1 (MQ=255)
ttGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCCTTCCANTCGTAANCATCAGCTTGTAAACGATagn > 1:903342/1‑75 (MQ=255)
cGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTg > 2:561390/1‑76 (MQ=255)
cGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTg > 1:590934/1‑76 (MQ=255)
cATTCATGTTTATTGGCCACGACCGGGTCCTTTCCACTCGTAAACATCAGCTTGTACACGATAGAGTGCTTAAggn > 1:924344/1‑75 (MQ=255)
tCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCtt > 2:606116/1‑76 (MQ=255)
cATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTc > 1:597260/1‑76 (MQ=255)
aTGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCt < 2:102401/76‑1 (MQ=255)
tGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTa < 2:194514/76‑1 (MQ=255)
tttATTGGCGACGACCGGGTCCTATCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACg > 2:633318/1‑76 (MQ=255)
ttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGCAAACGATAGAGTGCTTAAGGGCTTCTACGc > 2:558053/1‑76 (MQ=255)
tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGANAGAGTGCTTAAGGGCTTCTACGCt < 2:177285/76‑1 (MQ=255)
gCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCg < 1:23905/76‑1 (MQ=255)
cgacgaCCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGt > 2:478111/1‑76 (MQ=255)
cgacgaCCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGt < 2:863077/76‑1 (MQ=255)
gacgacCGGGTCCTTTCNAACCGTAAACATCAGCTTGTAAACGATAGAGTGNGTAATGCCTGCGACGCTAAGCGTg > 2:890016/1‑76 (MQ=255)
gacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTAGGc < 1:245262/76‑1 (MQ=255)
ccGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGa > 1:411894/1‑76 (MQ=255)
ccGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGa < 2:638504/76‑1 (MQ=255)
cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGAt > 2:795391/1‑76 (MQ=255)
gggTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATg < 2:333283/76‑1 (MQ=255)
gggTCCTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATg < 2:817026/76‑1 (MQ=255)
cTTTCCAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAatat < 1:666017/76‑1 (MQ=255)
ccAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTaa < 2:82572/76‑1 (MQ=255)
cAATCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTaaa > 2:38682/1‑76 (MQ=255)
tCGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACgg < 1:413308/76‑1 (MQ=255)
cGTAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGa < 1:623759/76‑1 (MQ=255)
tAAACATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGc > 1:545266/1‑76 (MQ=255)
aaaCATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCa > 2:467908/1‑76 (MQ=255)
aaaCATCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCa > 1:156598/1‑76 (MQ=255)
aTCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCa > 1:629145/1‑76 (MQ=255)
aTCAGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCa < 1:403166/76‑1 (MQ=255)
aGCTTGTAAACGATAGAGTGCTTANGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCatat > 2:170518/1‑76 (MQ=255)
aGCTTGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCatat < 1:175014/76‑1 (MQ=255)
ttGTAAANGATAGAGTGCTTAGTGNCTTCTACGCTAAGCGTNGGCGATGAGTATGTAAACGGAGCATTCATATAgg < 2:955481/76‑1 (MQ=255)
tGTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGc < 1:281717/76‑1 (MQ=255)
gTAAACGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCg > 1:272158/1‑76 (MQ=255)
|
TAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCG > REL606/3497898‑3498042
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
