Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
17 |
21.6 |
1356596 |
98.4% |
1334890 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,894,119 |
C→A |
intergenic (‑8/+89) |
proQ ← / ← yebR |
putative solute/DNA competence effector/hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,894,119 | 0 | C | A | 100.0%
| 76.5
/ NA
| 22 | intergenic (‑8/+89) | proQ/yebR | putative solute/DNA competence effector/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (14/8); total (14/8) |
AAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTACC > REL606/1894049‑1894183
|
aaaaCGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGAt > 2:248925/1‑76 (MQ=255)
aaCGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTa > 1:359229/1‑76 (MQ=255)
ggCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGa > 2:25131/1‑76 (MQ=255)
aGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTa > 1:670839/1‑76 (MQ=255)
gAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAg > 1:168384/1‑76 (MQ=255)
aCGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCca < 2:133678/76‑1 (MQ=255)
aCGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCca < 1:567841/76‑1 (MQ=255)
gATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCcaacaa > 1:339757/1‑76 (MQ=255)
ttACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAgc > 1:206344/1‑76 (MQ=255)
ttACTTCTTTACTGCTATTCAAATTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAgc > 2:546375/1‑76 (MQ=255)
aCTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAgcgc < 1:550186/76‑1 (MQ=255)
tACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATg > 2:329689/1‑76 (MQ=255)
aCTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGa < 1:631332/76‑1 (MQ=255)
aCTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGa < 2:523228/76‑1 (MQ=255)
tGCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAAc < 1:264426/76‑1 (MQ=255)
gCTATTCAACTTAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACa > 2:630995/1‑76 (MQ=255)
tAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATtat > 2:223287/1‑76 (MQ=255)
tAGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATtat > 2:341397/1‑76 (MQ=255)
aGGTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATtata < 1:373870/76‑1 (MQ=255)
gTTGATTTTCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAAt < 1:31319/76‑1 (MQ=255)
tttCCATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCt > 1:557048/1‑76 (MQ=255)
ccATGAAATTTACTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTAcc > 2:484697/1‑76 (MQ=255)
|
AAAACGTTCGGCCAGAAACGCGATTACTTCTTTACTGCTATTCAACTTAGGTTGATTTTCCATGAAATTTCCTGATTACAACGGACGTAGCCAACAAGCGCAGGCATGAACAGGCGTCATTATAATGACGCTACC > REL606/1894049‑1894183
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
