Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F1 I1 R1 17 21.6 1356596 98.4% 1334890 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
JC REL606 2,236,113 (TTATCGCCT)2→3 coding (550/1761 nt) yejM → predicted hydrolase, inner membrane

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2236095 =2 (0.090)19 (1.020) 19/130 0.1 95.7% coding (532/1761 nt) yejM predicted hydrolase, inner membrane
?REL606 = 2236103 0 (0.000)coding (540/1761 nt) yejM predicted hydrolase, inner membrane

TTAGCGCGCTGCATGGTGATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2236177‑2236095
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aggcgataaaGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCTGCGCAGCTTTTGCCAG  <  REL606/2236103‑2236031
                                                                                                                                                            
TTAGCGCGCTGCATGGTGATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGn                                                                                  <  1:24591/76‑2
TTAGCGCGCTGCATGGTGATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGG                                                                                  >  2:224947/1‑76
    CGCGCTGCATGGTGATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGAT                                                                              >  2:611123/1‑76
       GCTGCATGGTGATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAA                                                                           >  1:666963/1‑76
          GCATGGTGATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAAGGC                                                                        >  2:243151/1‑76
                TGATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAA                                                                  <  1:579360/76‑1
                 GATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAAT                                                                 <  1:418566/76‑1
                    CGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAG                                                              <  1:206540/76‑1
                     GGGCGATAGAAGTTGGCATCGGACCAGATATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGA                                                             >  2:113814/1‑76
                         GATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGC                                                         <  1:537377/76‑1
                              AAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCA                                                    <  1:618759/76‑1
                                      ATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGC                                            >  2:96060/1‑76
                                               GATATACACCTCATCCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGTGAAGCGT                                   <  1:237413/76‑1
                                                ATATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTC                                  >  2:545895/1‑76
                                                 TATACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAGGCGTCG                                 <  2:481405/76‑1
                                                    ACACCACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACG                              >  2:544408/1‑76
                                                         ACATGCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCG                         >  2:77738/1‑76
                                                             GCGAGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAG                     <  2:440296/76‑1
                                                                AGGCGATAAAGGCGTTAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCT                  <  2:134786/76‑1
                                                                AGGCGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCT                  >  2:173012/1‑76
                                                                   CGATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCTGCG               >  2:529386/1‑76
                                                                    GATAAAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCTGCGC              <  2:669116/76‑1
                                                                        AAGGCGATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCTGCGCAGCT          >  1:541311/1‑76
                                                                              ATAAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCTGCGCAGCTTTTGCC    <  2:506598/76‑1
                                                                                AAAGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCTGCGCAGCTTTTGCCAG  >  2:369828/1‑76
                                                                                                                                                            
TTAGCGCGCTGCATGGTGATCGGGCGATAGAAGTTGGCATCGGCCCAGATATACACCACATGCGAGGCGATAAAGGCGATAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2236177‑2236095
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aggcgataaaGGCGATAAATAAGAATGCGGCCAGCGGGCGCGCGAAGCGTCGACGACGCGTCAGGCTGCGCAGCTTTTGCCAG  <  REL606/2236103‑2236031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.

GATK/CNVnator alignment

N/A