Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
17 |
21.6 |
1356596 |
98.4% |
1334890 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,237,516 |
(T)7→6 |
intergenic (+41/+62) |
proL → / ← yejO |
tRNA‑Pro/predicted autotransporter outer membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,237,510 | 0 | T | . | 96.0%
| 106.3
/ ‑2.4
| 25 | intergenic (+35/+68) | proL/yejO | tRNA‑Pro/predicted autotransporter outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (8/16); total (8/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 |
GGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAG > REL606/2237441‑2237581
|
gggggTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGtttttta > 1:599191/1‑75 (MQ=255)
gTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATc > 2:276035/1‑76 (MQ=255)
cGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTg < 2:32102/76‑1 (MQ=255)
ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGc < 1:291224/76‑1 (MQ=255)
ggAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGc > 2:167094/1‑76 (MQ=255)
aGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCaa < 2:260091/76‑1 (MQ=255)
gTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCaatt < 2:47304/76‑1 (MQ=255)
aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCaattaatt < 2:462062/76‑1 (MQ=255)
aaaTCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCaattaatt < 2:140633/76‑1 (MQ=255)
tctcGTGCCTACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATa < 1:43978/76‑1 (MQ=255)
tctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATTTGCAATTAATTCGATaa > 2:272749/1‑76 (MQ=255)
ctcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACTGTTGG‑TTTTTTATATCTGCAATTAATTCGATaaa < 2:157299/76‑1 (MQ=255)
tcGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAAc < 1:111958/76‑1 (MQ=255)
cGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACa < 1:497103/76‑1 (MQ=255)
tGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGa > 2:631810/1‑76 (MQ=255)
aCCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTg < 2:119675/76‑1 (MQ=255)
aCCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTg > 2:275589/1‑76 (MQ=255)
aaaaTCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGacac > 2:467808/1‑76 (MQ=255)
aaaTCCCAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGAcaca < 2:413134/76‑1 (MQ=255)
ccAAGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATcaca < 1:343166/76‑1 (MQ=255)
aaGAAAAAACCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGc > 1:481283/1‑76 (MQ=255)
aaaaaaCCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTg < 1:375784/76‑1 (MQ=255)
aaaaaCCAACCCTTACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGt < 2:502585/76‑1 (MQ=255)
ttACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGtt > 1:52597/1‑76 (MQ=255)
ttACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGtt < 1:397123/76‑1 (MQ=255)
ttACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGtt < 1:397113/76‑1 (MQ=255)
ttACGGTTGG‑TTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGtt < 2:432959/76‑1 (MQ=255)
ggttgg‑ttTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCa < 1:189430/76‑1 (MQ=255)
gttgg‑ttTTTTATATCTGCAATTAATTCGATAAACAGACCGTTACACATCACAGCCTGTTTATTTTCTGTTATCAg < 1:87836/76‑1 (MQ=255)
gttgg‑ttTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAg < 1:202747/76‑1 (MQ=255)
|
GGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCAAAAATCCCAAGAAAAAACCAACCCTTACGGTTGGTTTTTTTATATCTGCAATTAATTCGATAAACAGACCGTGACACATCACAGCCTGTTTATTTTCTGTTATCAG > REL606/2237441‑2237581
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
