Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
14 |
23.9 |
1506608 |
98.1% |
1477982 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,168,340 |
A→C |
E1127A (GAA→GCA) |
rpoC → |
DNA‑directed RNA polymerase subunit beta' |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,168,340 | 0 | A | C | 100.0%
| 86.8
/ NA
| 22 | E1127A (GAA→GCA) | rpoC | DNA‑directed RNA polymerase subunit beta' |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/13); total (9/13) |
TAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGC > REL606/4168269‑4168400
|
tAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATcc > 2:127760/1‑76 (MQ=255)
aaaGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCcg > 2:475936/1‑76 (MQ=255)
aTTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTa < 2:672230/76‑1 (MQ=255)
tGTTCAGCTGGAAGATGGCGTACAGATCAACTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTAcc < 1:146912/76‑1 (MQ=255)
gTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCa > 2:545009/1‑76 (MQ=255)
ttCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCaa < 1:44384/76‑1 (MQ=255)
ttCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCaa < 2:353795/76‑1 (MQ=255)
gCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGTGTATTCCGCAGGCATCCGGCGGTACCAAGGAc < 1:317045/76‑1 (MQ=255)
gAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCa < 1:508240/76‑1 (MQ=255)
tGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCggt < 2:669181/76‑1 (MQ=255)
ggCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCggtg > 1:685159/1‑76 (MQ=255)
ggCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCggtg > 2:446501/1‑76 (MQ=255)
tACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCt < 1:230411/76‑1 (MQ=255)
ctctGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGtt < 2:746172/76‑1 (MQ=255)
tGGTGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCg < 2:121618/76‑1 (MQ=255)
tGACACCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGAc > 2:667955/1‑76 (MQ=255)
acacCCTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGGTGCGGACCt > 2:67357/1‑76 (MQ=255)
cacCCTGGCGCGTATTCCGCAGGCATCCGTCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTg < 2:231630/76‑1 (MQ=255)
cccTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGtt < 2:351350/76‑1 (MQ=255)
ccTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGAGGACCTGTTc > 2:160817/1‑76 (MQ=255)
cTGGCGCGTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGTGGACCTGTTCg < 2:448278/76‑1 (MQ=255)
cgcgTATTCCGCAGGCATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGc > 1:376207/1‑76 (MQ=255)
|
TAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACACCCTGGCGCGTATTCCGCAGGAATCCGGCGGTACCAAGGACATCACCGGTGGTCTGCCGCGCGTTGCGGACCTGTTCGAAGC > REL606/4168269‑4168400
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
