Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A25 F1 I1 R1
|
14 |
46.3 |
3075166 |
96.7% |
2973685 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,873,434 |
T→G |
T201P (ACC→CCC) |
glmS ← |
D‑fructose‑6‑phosphate amidotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,873,434 | 0 | T | G | 97.2%
| 139.4
/ ‑6.7
| 36 | T201P (ACC→CCC) | glmS | D‑fructose‑6‑phosphate amidotransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (18/17); total (18/18) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAG > REL606/3873361‑3873500
|
ttATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCa > 1:839322/1‑76 (MQ=255)
aTCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCAcc > 2:1095307/1‑76 (MQ=255)
aTCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCAcc > 2:936272/1‑76 (MQ=255)
tCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCg < 1:453967/76‑1 (MQ=255)
tCGAAGATGTTTACCGAACGACNNGTGATTTCCGNNTTATCGCCCTCTTCGAGGAAGATAAAGCGACGGGGCACCg < 1:1380181/76‑1 (MQ=255)
cGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCgg > 1:1329668/1‑76 (MQ=255)
gAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGc < 2:1014976/76‑1 (MQ=255)
gAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGc < 2:1014970/76‑1 (MQ=255)
tGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAg < 2:93993/76‑1 (MQ=255)
aCCGAACGGCGAGTGCTTTCCTTAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCa < 2:46138/76‑1 (MQ=255)
aCCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCa < 2:653457/76‑1 (MQ=255)
ccGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCGAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAg < 2:1360717/76‑1 (MQ=255)
gAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGcc > 2:146903/1‑75 (MQ=255)
aaCGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTg < 2:37618/76‑1 (MQ=255)
aCGGCGAGTGAGTTCCGCAATATCGCCCGCTTCAAGGAAGATAAAGCGTCGGGGCACCGGCAACAGCGCCAGCTgg < 2:131175/76‑1 (MQ=255)
gAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGa > 1:1115632/1‑76 (MQ=255)
gAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGa > 1:1023666/1‑76 (MQ=255)
aGTGATTTCCGCAATATCGCCCTCTTCCAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGCTCAGaa > 1:1510478/1‑76 (MQ=255)
gTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAg < 1:892008/76‑1 (MQ=255)
gATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCg < 2:864347/76‑1 (MQ=255)
tttCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGAt > 1:1117469/1‑76 (MQ=255)
ttCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATa > 2:568170/1‑76 (MQ=255)
cAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtt > 2:475204/1‑76 (MQ=255)
atatCGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt < 2:827032/76‑1 (MQ=255)
cGCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGcc > 2:416218/1‑76 (MQ=255)
gCCCTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGccc > 1:1129492/1‑76 (MQ=255)
cccTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCa < 2:1364795/76‑1 (MQ=255)
ccTCTTCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCAt > 1:1115385/1‑76 (MQ=255)
ttCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc < 2:1534528/76‑1 (MQ=255)
ttCAAGGAAGATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATcccc > 1:650941/1‑76 (MQ=255)
aaGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCaa < 2:650684/76‑1 (MQ=255)
gATAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATc < 2:1107339/76‑1 (MQ=255)
tAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCATGCCAATCAc > 1:54448/1‑76 (MQ=255)
tAAAGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAc < 1:1219709/76‑1 (MQ=255)
aaaGCGACGGGGCACCGGCAACAGCGCCAGCTGNTCAGAAGCGATAAAGTTTTCGCNCATCCCCAAGNCAATCAcc > 2:1276458/1‑76 (MQ=255)
aaGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCa > 2:589177/1‑76 (MQ=255)
aGCGACGGGGCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAg > 2:385360/1‑76 (MQ=255)
|
TTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAG > REL606/3873361‑3873500
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
