breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
RA | 183,037 | G→T | R320L (CGC→CTC) | dgt → | deoxyguanosinetriphosphate triphosphohydrolase |
RA | 1,290,249 | Δ1 bp | coding (214/1014 nt) | hnr → | response regulator of RpoS |
JC JC | 1,305,418 | IS150 (–) +2 bp | coding (821‑822/1461 nt) | cls ← | cardiolipin synthetase |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
JC | 2,103,915 | (CCAG)7→6 | coding (182‑185/216 nt) | ECB_01992 → | hypothetical protein |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,873,434 | T→G | T201P (ACC→CCC) | glmS ← | D‑fructose‑6‑phosphate amidotransferase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
MC JC | 3,912,149 | Δ15 bp | coding (28‑42/99 nt) | ilvL → | ilvG operon leader peptide |
RA | 4,163,752 | T→G | I966S (ATC→AGC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546976–547700 | 593392 | 45693–46417 | 17 [15] | [14] 16 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593568 | 619843–619116 | 25549–26276 | 17 [15] | [15] 17 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 4194708 = | 56 (1.150) | 50 (1.040) | 41/148 | 0.2 | 52.0% | intergenic (+54/‑215) | metA/aceB | homoserine O‑succinyltransferase/malate synthase |
? | REL606 | = 4449216 | 37 (0.770) | coding (242/1422 nt) | treB | fused trehalose(maltose)‑specific PTS enzyme: IIB component/IIC component |