breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 183,037 G→T R320L (CGC→CTC)  dgt → deoxyguanosinetriphosphate triphosphohydrolase
RA 1,290,249 Δ1 bp coding (214/1014 nt) hnr → response regulator of RpoS
JC JC 1,305,418 IS150 (–) +2 bp coding (821‑822/1461 nt) cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
JC 2,103,915 (CCAG)7→6 coding (182‑185/216 nt) ECB_01992 → hypothetical protein
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,873,434 T→G T201P (ACC→CCC)  glmS ← D‑fructose‑6‑phosphate amidotransferase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
MC JC 3,912,149 Δ15 bp coding (28‑42/99 nt) ilvL → ilvG operon leader peptide
RA 4,163,752 T→G I966S (ATC→AGC)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546976–547700 593392 45693–46417 17 [15] [14] 16 [insB‑6]–[fepA] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593568 619843–619116 25549–26276 17 [15] [15] 17 [fepA]–insA‑8 [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 4194708 =56 (1.150)50 (1.040) 41/148 0.2 52.0% intergenic (+54/‑215) metA/aceB homoserine O‑succinyltransferase/malate synthase
?REL606 = 4449216 37 (0.770)coding (242/1422 nt) treB fused trehalose(maltose)‑specific PTS enzyme: IIB component/IIC component