Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A79 F1 I1 R1
|
19 |
52.0 |
3333102 |
95.7% |
3189778 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,096,697 |
(T)8→7 |
coding (8/1509 nt) |
glpK ← |
glycerol kinase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,096,690 | 0 | T | . | 94.1%
| 143.9
/ 3.6
| 34 | coding (15/1509 nt) | glpK | glycerol kinase |
| Reads supporting (aligned to +/- strand): ref base T (2/0); new base . (15/17); total (17/17) |
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 |
CTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTT > REL606/4096616‑4096757
|
cTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtt < 1:33008/76‑1 (MQ=255)
cTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtt > 2:1559768/1‑76 (MQ=255)
tAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAttt > 2:842331/1‑76 (MQ=255)
aaTGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATAtttt < 2:870290/76‑1 (MQ=255)
tGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTc < 1:1270221/76‑1 (MQ=255)
ggCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCa < 1:804129/76‑1 (MQ=255)
gATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAAt > 2:1114872/1‑76 (MQ=255)
aTCCATTACGACCGCGCGGGAGCGGGATGTGCCCTGGTCGAGCGCTACGATGGCTTTTTTTTCCCTCCTGTTTAAt > 2:653298/1‑76 (MQ=255)
ccATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGt > 1:390012/1‑76 (MQ=255)
ccATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGt < 1:67259/76‑1 (MQ=255)
cATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGt > 1:1116870/1‑76 (MQ=255)
cATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTc > 2:1251630/1‑76 (MQ=255)
aCGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCTGt > 2:197518/1‑76 (MQ=255)
aCGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCga < 2:1411931/76‑2 (MQ=255)
aCGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGt < 2:976665/76‑1 (MQ=255)
ccGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTc < 2:1286140/76‑1 (MQ=255)
cgcgGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCata < 2:451498/76‑1 (MQ=255)
cgGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATAtt > 2:583223/1‑76 (MQ=255)
cgGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATAtt < 1:415679/76‑1 (MQ=255)
cgGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATAtt > 2:379219/1‑76 (MQ=255)
ggAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTAc < 2:79674/76‑1 (MQ=255)
tggtggTGCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGa > 1:1449957/1‑76 (MQ=255)
tggtgCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGc < 2:927227/76‑1 (MQ=255)
gCCCTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCtttt > 1:1523982/1‑76 (MQ=255)
cccTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGGGAAGCtttta < 2:1248764/76‑2 (MQ=255)
cccTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCttttt > 1:985678/1‑76 (MQ=255)
ccTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTg > 1:1471236/1‑76 (MQ=255)
ccTGGTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTg < 2:853036/76‑1 (MQ=255)
gTCGAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCt < 1:422962/76‑1 (MQ=255)
gAGCGCAACGATATATTTTTTTTCAGTCATGGTTAATTGTCCCGTAGTCATATTACAGCGAAGGTTTTTGTTCTGa < 2:72236/76‑1 (MQ=255)
gAGCGCAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGaa > 1:444102/1‑76 (MQ=255)
gcgcAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAgg < 2:736794/76‑1 (MQ=255)
gcgcAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAgg > 2:921547/1‑76 (MQ=255)
gcgcAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAgg < 2:985678/76‑1 (MQ=255)
gcgcAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCAGATTACAGCGAAGCTTTTTGTTCTGAAgg < 2:348933/76‑1 (MQ=255)
cAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGCAGGAGt > 2:1408358/1‑76 (MQ=255)
cAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGt < 1:655670/76‑1 (MQ=255)
cAACGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGt > 2:1015825/1‑76 (MQ=255)
aaCGATATA‑TTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGtt > 2:1557942/1‑76 (MQ=255)
aCGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGtt > 2:665197/1‑76 (MQ=255)
|
CTAATGATATTGGCATCGTGATCCATTACGACCGCGCGGGAGCTGGTGGTGCCCTGGTCGAGCGCAACGATATATTTTTTTTCAGTCATGTTTAATTGTCCCGTAGTCATATTACAGCGAAGCTTTTTGTTCTGAAGGAGTT > REL606/4096616‑4096757
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
