breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 450,008 A→T I226N (ATC→AAC)  ylaB ← conserved inner membrane protein
JC 474,713 (TAATGGCGATAAATG)1→2 coding (593/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
MC JC 1,289,460 Δ611 bp [ychK][hnr] [ychK], [hnr]
RA 1,305,392 T→C E283G (GAA→GGA)  cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,591,144 T→C N158S (AAT→AGT)  ydeH ← hypothetical protein
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,222,511 C→T R200C (CGT→TGT)  rtn → hypothetical protein
RA 2,520,689 (T)8→7 intergenic (‑101/‑45) dapA ← / → gcvR dihydrodipicolinate synthase/DNA‑binding transcriptional repressor, regulatory protein accessory to GcvA
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,911,964 2 bp→TT intergenic (‑165/‑157) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
JC 3,928,221 (CACTATGAA)1→2 coding (5/1260 nt) rho → transcription termination factor Rho
RA 4,096,697 (T)8→7 coding (8/1509 nt) glpK ← glycerol kinase
RA 4,166,077 G→A A373T (GCA→ACA)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA 4,202,538 T→G K8Q (AAA→CAA)  iclR ← DNA‑binding transcriptional repressor
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546986–547700 593332 45633–46347 17 [13] [14] 15 [insB‑6]–[fepA] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593585 619827–619116 25532–26243 15 [14] [14] 15 [fepA]–[insA‑8] [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8]