Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A68 F1 I1 R1
|
17 |
56.4 |
3914180 |
96.2% |
3765441 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,442,003 |
C→A |
A265E (GCG→GAG) |
pmbA → |
predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,442,003 | 0 | C | A | 100.0%
| 282.8
/ NA
| 71 | A265E (GCG→GAG) | pmbA | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (35/36); total (35/36) |
ACCATGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGCGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCT > REL606/4441930‑4442075
|
accaTGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAgg > 2:1219306/1‑76 (MQ=255)
ccaTGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAggg > 2:536696/1‑76 (MQ=255)
ccaTGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAggg < 2:542743/76‑1 (MQ=255)
tGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTgg < 2:1879170/76‑1 (MQ=255)
tGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTgg > 1:228843/1‑76 (MQ=255)
aaaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCTATAGAGGGTGGCt < 2:1824574/76‑1 (MQ=255)
aaaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCt < 2:813434/76‑1 (MQ=255)
aaaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCt < 2:204053/76‑1 (MQ=255)
aaaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCt > 2:790133/1‑76 (MQ=255)
aaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTc > 2:447231/1‑76 (MQ=255)
gcgcCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCgg < 1:1606010/76‑1 (MQ=255)
gcgcCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCgg < 1:28430/76‑1 (MQ=255)
ccAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGttt < 2:1736843/76‑1 (MQ=255)
ccAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGttt < 1:585595/76‑1 (MQ=255)
cAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTa < 1:971318/76‑1 (MQ=255)
cAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTa < 1:800073/76‑1 (MQ=255)
cAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTa < 1:1592939/76‑1 (MQ=255)
tCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCg < 2:713241/76‑1 (MQ=255)
cATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGt > 2:1607251/1‑76 (MQ=255)
cATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGt > 1:1033289/1‑76 (MQ=255)
aTTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTa > 2:743469/1‑76 (MQ=255)
aTTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGGTTACCGTa > 2:912981/1‑76 (MQ=255)
tttttGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTaa < 2:1864609/76‑1 (MQ=255)
ttttGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGCaaa > 1:1481407/1‑76 (MQ=255)
tttGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAAt > 2:160027/1‑76 (MQ=255)
tttGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAAt < 2:220198/76‑1 (MQ=255)
aaTGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCt > 2:1208737/1‑76 (MQ=255)
aaTGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCt > 2:402835/1‑76 (MQ=255)
tGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCttt > 2:1467536/1‑76 (MQ=255)
tGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCttt < 2:1817771/76‑1 (MQ=255)
tGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCttt < 2:1728181/76‑1 (MQ=255)
gAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTc > 2:590525/1‑76 (MQ=255)
aaGTGGCGACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTct < 2:802605/76‑1 (MQ=255)
tGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTctgct > 2:1307327/1‑76 (MQ=255)
tGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTctgct < 2:1450680/76‑1 (MQ=255)
tGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTctgct > 2:523347/1‑76 (MQ=255)
gcgaCCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTgg < 1:1891768/73‑1 (MQ=255)
aaCCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGan < 1:44326/76‑2 (MQ=255)
ccGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTc < 1:1870783/76‑1 (MQ=255)
gggCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGc > 2:1066721/1‑76 (MQ=255)
gggCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGc > 2:871717/1‑76 (MQ=255)
gggCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGc > 2:897096/1‑76 (MQ=255)
ggCTTTTTGCCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCt < 2:971697/76‑1 (MQ=255)
tttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTggg > 1:383908/1‑76 (MQ=255)
ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt < 1:918888/76‑1 (MQ=255)
ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt > 1:826130/1‑76 (MQ=255)
ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt < 1:177795/76‑1 (MQ=255)
ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt < 1:582298/76‑1 (MQ=255)
ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt < 1:582274/76‑1 (MQ=255)
tttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTa < 2:1103116/76‑1 (MQ=255)
tttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTa > 1:1262739/1‑76 (MQ=255)
tttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTa < 2:1375496/76‑1 (MQ=255)
tGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTaaa < 1:1041174/76‑1 (MQ=255)
tGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTaaa < 1:1024026/76‑1 (MQ=255)
cATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACaaa > 1:707865/1‑76 (MQ=255)
tggtggGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCt > 1:689707/1‑76 (MQ=255)
gtggGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGc > 1:1839194/1‑76 (MQ=255)
tggGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGcc > 2:1190226/1‑76 (MQ=255)
gggggCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCg > 1:280470/1‑76 (MQ=255)
gggggCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCg < 1:177751/76‑1 (MQ=255)
gggggCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCg > 1:1102666/1‑76 (MQ=255)
gCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACt < 1:623189/76‑1 (MQ=255)
cGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTg < 1:569075/76‑1 (MQ=255)
gATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTgg > 1:1604586/1‑76 (MQ=255)
aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGc > 1:48439/1‑76 (MQ=255)
aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGc > 1:261708/1‑76 (MQ=255)
aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGc > 1:771200/1‑76 (MQ=255)
aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGc < 2:1694677/76‑1 (MQ=255)
aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGCAAACAAATTCTCCCGGACTGGc > 1:549694/1‑76 (MQ=255)
tAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCt < 2:1909541/76‑1 (MQ=255)
tAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCt > 1:1021598/1‑76 (MQ=255)
|
ACCATGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGCGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCT > REL606/4441930‑4442075
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
