Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I1 R1
|
18 |
29.2 |
1933165 |
95.1% |
1838439 |
75.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,028,040 |
A→T |
L141* (TTA→TAA) |
ECB_02828 ← |
Polysialic acid transport protein kpsM |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,028,040 | 0 | A | T | 100.0%
| 118.0
/ NA
| 32 | L141* (TTA→TAA) | ECB_02828 | Polysialic acid transport protein kpsM |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (14/18); total (14/18) |
CCAACGACCATGAATATTAAGCCGACGCCACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTAAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAACAGCAACGT > REL606/3027968‑3028112
|
ccAACAACCATGAATATTAAGCCGACCCCACATNAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTtaaa < 1:772396/76‑1 (MQ=255)
cAACGACCATGAATATTAAGCCGACGCCACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTtaaaa < 2:250605/76‑1 (MQ=255)
ccATGAATATTAAGCCGACGCCACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGt > 1:490274/1‑76 (MQ=255)
tatTAAGCCGACGCCACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCg > 1:412627/1‑76 (MQ=255)
tatTAAGCCGACGCCACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGATATCTCg < 1:643367/76‑1 (MQ=255)
tgaaGCGGACGCCACATGAAAGNATGATCAGCANGCTCCAGGNAGCCACAAGTCGTTAAAATTTTNTTATCTCGaa < 2:838528/74‑1 (MQ=255)
aGCCGACGCCACATGAAAGATCGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAata < 2:810114/76‑1 (MQ=255)
gCCGACGCCACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAAGTTGTTATCTCGAAatat < 2:40117/76‑1 (MQ=255)
gCCACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCt < 1:876258/76‑1 (MQ=255)
ccACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTg > 2:96684/1‑76 (MQ=255)
tGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTACAAATTTGTTATCTCGAAATATTCGCCTGCCATc > 1:175898/1‑76 (MQ=255)
gAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATcc < 1:786273/76‑1 (MQ=255)
gAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATcc < 1:935492/76‑1 (MQ=255)
gAAAGAATAATCAGCAGGCTCCAGGTAGCCACAAATTGTTAAAAATTTGTTATCTCGAAATATTCGCCCGCCATcc < 2:865227/76‑1 (MQ=255)
aGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGa < 1:250790/76‑1 (MQ=255)
gAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGAc > 2:465483/1‑76 (MQ=255)
aTGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACaa < 1:518432/76‑1 (MQ=255)
aTGATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACaa < 1:934325/76‑1 (MQ=255)
aTGATCAGCACGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACaa > 1:126616/1‑76 (MQ=255)
gATCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATg < 1:295389/76‑1 (MQ=255)
aTCAGCAGGCTCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGa > 1:924476/1‑76 (MQ=255)
tCCAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAg > 2:545990/1‑76 (MQ=255)
ccAGGTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGa > 1:60491/1‑76 (MQ=255)
aGGTAGCNACAAGTTGTTAAACCTNTGTTATCTCGAAATATNCGCCTGCCTTCCAGACAATGAGCATATGCAGAat < 2:852655/76‑1 (MQ=255)
ggTAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAata < 2:77301/76‑1 (MQ=255)
tAGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAAtata > 1:321851/1‑76 (MQ=255)
aGCCACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATaa > 1:250052/1‑76 (MQ=255)
ccACAAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAAc < 2:220713/76‑1 (MQ=255)
ccACAAGTTGTTAAAAATTTGTTATCTAGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAAc < 1:581811/76‑1 (MQ=255)
acaAGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAACAg > 2:730821/1‑76 (MQ=255)
aaGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAACAGCa > 2:334261/1‑76 (MQ=255)
aaGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAACAGCa < 2:104427/76‑1 (MQ=255)
aaGTTGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAACAGCa < 1:798022/76‑1 (MQ=255)
tGTTAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAACAGCAACGt > 1:469370/1‑76 (MQ=255)
|
CCAACGACCATGAATATTAAGCCGACGCCACATGAAAGAATGATCAGCAGGCTCCAGGTAGCCACAAGTTGTAAAAAATTTGTTATCTCGAAATATTCGCCTGCCATCCAGACAATGAGCATAAGCAGAATATAAACAGCAACGT > REL606/3027968‑3028112
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
