Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A80 F1 I1 R1
|
17 |
48.4 |
3161007 |
95.9% |
3031405 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,451,762 |
T→A |
I144F (ATC→TTC) |
acpD ← |
acyl carrier protein phosphodiesterase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,451,762 | 0 | T | A | 97.9%
| 166.2
/ NA
| 48 | I144F (ATC→TTC) | acpD | acyl carrier protein phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (15/32); minor base C (0/1); total (15/33) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAG > REL606/1451689‑1451832
|
tCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAAcc < 1:1278012/76‑1 (MQ=255)
cGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAAccc > 1:258965/1‑76 (MQ=255)
cGGTAATGCCGATAAAGCCGAGGAACGTGGACAGANACGGCGTCACCAGGTCCGTTGGNCCATCTTTGTGGAAccc > 1:798665/1‑76 (MQ=255)
ggTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccg < 2:1129782/76‑1 (MQ=255)
gTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgc > 2:262146/1‑76 (MQ=255)
tAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgcc < 2:865519/76‑1 (MQ=255)
tAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgcc > 1:797888/1‑76 (MQ=255)
tAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgcc > 1:708778/1‑76 (MQ=255)
tAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgcc < 1:430547/76‑1 (MQ=255)
aaTGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgccg < 1:1015548/76‑1 (MQ=255)
aaTGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgccg < 2:1028007/76‑1 (MQ=255)
aaTGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACccgccg < 1:913178/76‑1 (MQ=255)
tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCcgcg < 1:1537269/76‑1 (MQ=255)
tGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCcgcg < 1:88214/76‑1 (MQ=255)
cGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCt < 2:1557575/76‑1 (MQ=255)
gaaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGt < 2:778435/75‑1 (MQ=255)
aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGta < 2:343234/76‑1 (MQ=255)
aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGta < 2:169479/76‑1 (MQ=255)
aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGta < 2:1252209/76‑1 (MQ=255)
aaaGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGta < 1:217222/76‑1 (MQ=255)
aGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGtaat < 1:1184317/76‑1 (MQ=255)
gCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGtaata < 1:2811/76‑1 (MQ=255)
gAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATg < 1:426178/76‑1 (MQ=255)
aCAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCtttttt < 2:1356460/76‑1 (MQ=255)
aCAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCtttttt < 1:253838/76‑1 (MQ=255)
aCAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCtttttt < 2:1332198/76‑1 (MQ=255)
aCAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCtttttt > 1:592485/1‑76 (MQ=255)
tACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCg < 2:436012/76‑1 (MQ=255)
aCGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGt > 2:221513/1‑76 (MQ=255)
aCGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTAACCGt > 2:392297/1‑76 (MQ=255)
cGGCGTCCCCAGGTCCGTTGGTCCTTCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGtt < 1:418806/76‑1 (MQ=255)
cGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGtt > 1:56948/1‑76 (MQ=255)
cGTCCCCAGGTCCGTTGGTCCATCTTTGTGGACCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTAcc < 1:723883/76‑1 (MQ=255)
cGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTAcc < 2:520165/76‑1 (MQ=255)
cGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTAcc < 1:418795/76‑1 (MQ=255)
cGTCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTAcc < 1:1010361/76‑1 (MQ=255)
tCACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAg > 1:937063/1‑76 (MQ=255)
cACCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGa < 1:465978/76‑1 (MQ=255)
aCCAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGAc > 1:347924/1‑76 (MQ=255)
ccAGGTCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGAcc > 2:1369461/1‑76 (MQ=255)
ntCCGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGACGGCTTTTTTACCCGTTACCAGACCATcc > 1:1579925/2‑76 (MQ=255)
ccGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCgg < 1:44251/76‑1 (MQ=255)
ccGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCgg > 2:245430/1‑76 (MQ=255)
cGTTGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGCTGGCTTTTTTACCCCTTACCAGACCTCCCGGa > 1:764110/1‑76 (MQ=255)
tGGTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCg < 1:1320721/76‑1 (MQ=255)
ggTCCATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGt > 1:109484/1‑76 (MQ=255)
ccATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTtct < 2:975276/76‑1 (MQ=255)
cATCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTtctc > 1:738465/1‑76 (MQ=255)
aTCTTTGTGGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCg < 1:921559/76‑1 (MQ=255)
ttGTGGACCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGtat < 2:341421/76‑1 (MQ=255)
tgtgGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGtata > 2:1088845/1‑76 (MQ=255)
gtgGAACCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAg < 1:697785/76‑1 (MQ=255)
|
TCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAG > REL606/1451689‑1451832
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
