Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A72 F1 I1 R1
|
16 |
60.6 |
3810144 |
97.0% |
3695839 |
75.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,911,998 |
C→A |
intergenic (‑199/‑124) |
yifB ← / → ilvL |
predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,911,998 | 0 | C | A | 94.7%
| 202.6
/ 3.5
| 57 | intergenic (‑199/‑124) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (22/32); total (23/34) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 |
GAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAA > REL606/3911926‑3912069
|
gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑Aaaaaaaaaa < 2:460513/76‑2 (MQ=255)
aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAaaaaca > 1:1873537/1‑76 (MQ=255)
tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAAtt > 1:187527/1‑76 (MQ=255)
aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAAttt < 2:887557/76‑1 (MQ=255)
aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAAttt < 2:1690602/76‑1 (MQ=255)
cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTta < 1:1818857/76‑1 (MQ=255)
gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTtat < 1:1645556/76‑1 (MQ=255)
ggAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTatat > 2:3069/1‑76 (MQ=255)
ggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGaaa > 1:489222/1‑76 (MQ=255)
gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAat > 1:1330081/1‑76 (MQ=255)
gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATCTATATTGAAat < 2:1619792/76‑1 (MQ=255)
aacaacATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAatt < 2:601718/76‑1 (MQ=255)
aacaacATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAatt > 1:959467/1‑76 (MQ=255)
aacaacATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAatt > 1:503111/1‑76 (MQ=255)
acaacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAatta < 2:1137231/76‑1 (MQ=255)
caacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAattat > 2:1528670/1‑76 (MQ=255)
aaacaTTCATACTGAAATTGATTTTTTTTCCCTCACTA‑TTTAATTTT‑AAAAAAAAACAATTTATATTGAAattatt < 1:1323288/75‑1 (MQ=255)
aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAattatt > 1:1542511/1‑76 (MQ=255)
aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAattatt > 2:35822/1‑76 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAAttatta < 2:1162245/76‑1 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAAttatta < 1:1444021/76‑1 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAAttatta < 1:206944/76‑1 (MQ=255)
acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATCTTT‑AAAAAAAAACAATTTATATTGAAAttatta > 2:670332/1‑76 (MQ=255)
caTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaa < 2:1845685/76‑1 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaaa < 2:599099/76‑1 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaaa < 2:338494/76‑1 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaaa < 1:833301/76‑1 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaaa < 2:599094/76‑1 (MQ=255)
aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAAGTATTaaa < 2:153013/76‑1 (MQ=255)
tACTGAAATTGAATTTTTTTGACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGcat < 2:1609168/76‑1 (MQ=255)
tGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGcatcat < 2:1499826/76‑1 (MQ=255)
aaaTTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:1428496/76‑1 (MQ=255)
aaTTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:382436/76‑1 (MQ=255)
ttGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaatgg < 2:1326926/76‑4 (MQ=255)
ttGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaaaaa > 2:187012/1‑76 (MQ=255)
ttGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaaaaa > 2:417067/1‑76 (MQ=255)
tcactcacTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaa < 1:142570/76‑1 (MQ=255)
actcactATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaact < 2:33064/76‑3 (MQ=255)
ctcactATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAata > 1:571610/1‑76 (MQ=255)
ctcactATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAata > 1:571591/1‑76 (MQ=255)
cactATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATc < 2:1144325/76‑1 (MQ=255)
cactATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAGATCGGCCAAAAAATATc < 1:1580800/76‑1 (MQ=255)
actATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCt > 2:657695/1‑76 (MQ=255)
ctATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt < 2:148078/76‑1 (MQ=255)
ttttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:1877665/1‑76 (MQ=255)
ttttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:1686177/1‑76 (MQ=255)
atttattttTAAAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 2:936867/75‑1 (MQ=255)
tttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:780452/1‑76 (MQ=255)
tttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:1592336/1‑76 (MQ=255)
ttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt > 2:487754/1‑76 (MQ=255)
ttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt > 2:487763/1‑76 (MQ=255)
tattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa < 1:54658/76‑1 (MQ=255)
attttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAt < 1:1845425/76‑1 (MQ=255)
ttttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAtt < 1:1692752/76‑1 (MQ=255)
tttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAttt < 2:1785337/76‑1 (MQ=255)
tttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAttt < 1:1292564/76‑1 (MQ=255)
ttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTa > 1:1286123/1‑76 (MQ=255)
ttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTa > 1:1480309/1‑76 (MQ=255)
ttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTa < 1:1589961/76‑1 (MQ=255)
tt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTAc < 1:1345323/76‑1 (MQ=255)
aaaaaaaaCAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaa > 1:1624284/1‑76 (MQ=255)
aaaaaaaaCAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaa > 1:882634/1‑76 (MQ=255)
aaaaaaaaCAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaa > 2:1112159/1‑76 (MQ=255)
aaaaaaACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaaa > 2:1322677/1‑76 (MQ=255)
|
GAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAA > REL606/3911926‑3912069
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
