breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
JC | 474,393 | Δ9 bp | coding (905‑913/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
RA | 650,773 | T→C | Y10C (TAT→TGT) | mrdA ← | transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2) |
RA | 977,118 | T→A | D405E (GAT→GAA) | aroA → | 3‑phosphoshikimate 1‑carboxyvinyltransferase |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
MC JC | 2,298,775 | (GCC)3→2 | coding (306‑308/1359 nt) | glpT ← | sn‑glycerol‑3‑phosphate transporter |
JC JC | 3,015,771 | IS150 (–) +3 bp | coding (1192‑1194/1677 nt) | ECB_02816 → | KpsD protein |
RA | 3,499,798 | T→G | T336P (ACC→CCC) | glgC ← | glucose‑1‑phosphate adenylyltransferase |
RA | 3,613,023 | A→C | intergenic (‑84/+99) | dctA ← / ← yhjK | C4‑dicarboxylate transport protein/predicted diguanylate cyclase |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,911,998 | C→A | intergenic (‑199/‑124) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
RA | 4,161,105 | G→A | E84K (GAA→AAA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546985–547700 | 593393 | 45694–46409 | 21 [19] | [20] 22 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593573 | 619839–619116 | 25544–26267 | 21 [18] | [20] 21 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |