breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,688,9900AC42.4% 39.5 / 13.5 33T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL6063,492,0820TG38.8% 79.6 / 16.8 49V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6064,574,2520TG36.7% 48.4 / 11.9 30S5S (TCA→TCChpaXhypothetical 4‑hydroxyphenylacetate permease
*REL6063,983,7000TG34.2% 64.8 / 23.1 41T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL6064,393,0610TG31.8% 87.9 / 12.2 44V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6061,795,6500AC29.0% 150.7 / 11.7 62V39G (GTG→GGG) celFcryptic phospho‑beta‑glucosidase, NAD(P)‑binding
*REL606716,8320TG27.5% 103.7 / 17.6 51A31A (GCA→GCCECB_00663hypothetical protein
*REL6062,631,7720AC27.1% 102.4 / 12.2 48intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*REL6062,296,3290AC26.2% 141.0 / 15.5 61intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6061,484,1050AG25.5% 108.7 / 12.0 47G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,875,4830GC22.7% 332.8 / 83.2 128intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL6061,747,9030GT20.0% 286.5 / 45.0 90A8S (GCA→TCA) ydiLhypothetical protein

Marginal new junction evidence (lowest skew 10 of 46 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2038074 =55 (0.920)8 (0.150) 7/134 5.6 12.7% coding (57/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 2038286 = 61 (1.140)coding (663/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 2041054 =67 (1.120)9 (0.170) 7/136 5.7 11.3% coding (330/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041468 = 81 (1.490)coding (1353/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 3022429 =76 (1.270)6 (0.110) 6/136 6.1 7.9% coding (256/1140 nt) ECB_02821 hypothetical protein
?REL606 3022636 = 71 (1.310)coding (49/1140 nt) ECB_02821 hypothetical protein
* ? REL606 = 358812386 (1.430)5 (0.090) 5/138 6.7 5.8% coding (267/528 nt) yhiE DNA‑binding transcriptional activator
?REL606 = 3588286 83 (1.500)coding (430/528 nt) yhiE DNA‑binding transcriptional activator
* ? REL606 3022554 =84 (1.400)6 (0.110) 5/138 6.7 6.1% coding (131/1140 nt) ECB_02821 hypothetical protein
?REL606 3022717 = 106 (1.920)intergenic (‑33/+541) ECB_02821/ECB_02822 hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
* ? REL606 = 203999965 (1.080)5 (0.090) 5/138 6.7 8.5% coding (130/438 nt) vioB VioB, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 = 2040151 48 (0.870)intergenic (‑23/+120) vioB/vioA VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 2040311 =67 (1.120)5 (0.090) 5/140 6.8 7.6% coding (1073/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2040458 = 59 (1.050)coding (926/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 = 203785991 (1.520)12 (0.210) 5/144 6.9 14.0% coding (272/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 = 2038290 60 (1.040)coding (659/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 2036228 =64 (1.070)7 (0.130) 4/130 6.9 10.7% coding (731/1113 nt) wbbC putative glycosyltransferase WbbC
?REL606 2036759 = 62 (1.190)coding (200/1113 nt) wbbC putative glycosyltransferase WbbC
* ? REL606 3021263 =91 (1.520)4 (0.080) 4/132 7.0 5.2% coding (221/933 nt) ECB_02820 hypothetical protein
?REL606 3021399 = 67 (1.270)coding (85/933 nt) ECB_02820 hypothetical protein