Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A31 F1 I1 R1
|
21 |
37.9 |
2424921 |
96.5% |
2340048 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,252,023 |
A→T |
D246E (GAT→GAA) |
nusA ← |
transcription elongation factor NusA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,252,023 | 0 | A | T | 100.0%
| 113.6
/ NA
| 31 | D246E (GAT→GAA) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (13/18); total (13/18) |
CCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTA > REL606/3251958‑3252092
|
ccACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGtt < 1:797795/76‑1 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGttt < 1:730248/76‑1 (MQ=255)
ccccAGTTCAGTAGACCCCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTa < 2:1186119/75‑1 (MQ=255)
ccccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTa < 1:546709/75‑1 (MQ=255)
aGTTCAGTAGAAACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGt > 2:299488/1‑76 (MQ=255)
ttCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTg > 1:763168/1‑76 (MQ=255)
tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTgg > 2:932688/1‑76 (MQ=255)
aGTANACACCGCCTGAACACGCGCGCCATGCATACCTACNCAAGCACCTACCGGTTCGATACGTTTATCGTTGGtt < 1:141747/76‑1 (MQ=255)
aGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGtt < 1:706250/76‑1 (MQ=255)
gTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGttt > 1:975597/1‑76 (MQ=255)
acacCGCCTGAACTCGCGCGCCATGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCAc < 1:1038127/76‑1 (MQ=255)
acacCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCAc < 1:399537/76‑1 (MQ=255)
acacCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCAc < 1:851689/76‑1 (MQ=255)
acCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACcg < 1:815429/76‑1 (MQ=255)
nccTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTNGGTTTTCACCGCGa > 2:174611/2‑76 (MQ=255)
cTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGAtt > 1:441371/1‑76 (MQ=255)
gAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGGtttt < 2:1197195/76‑1 (MQ=255)
gAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGAtttt < 2:945403/76‑1 (MQ=255)
gAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGAtttt < 2:385902/76‑1 (MQ=255)
gcgcgcCACGCATATCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTGACCGCGATTTTCGCACg < 2:176162/76‑1 (MQ=255)
cgcCACGCATACCTACGCAAGCACCTACCGTTTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACgaga < 2:108493/76‑1 (MQ=255)
gcCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTTACCGCGATTTTTGCCCgagga > 2:912692/1‑74 (MQ=255)
gcCACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGaa > 1:883548/1‑76 (MQ=255)
ccACGCATACCTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAc < 2:716497/76‑1 (MQ=255)
cTACGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcgcg > 2:749343/1‑76 (MQ=255)
aCGCAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAg > 2:1201212/1‑76 (MQ=255)
cAAGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCg > 1:832576/1‑76 (MQ=255)
aaGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgc > 1:801356/1‑76 (MQ=255)
aaGCACCTACCGGTTCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgc > 1:631413/1‑76 (MQ=255)
gCACCTCCGGGTTCGATACGTTTATCGTGGGTTTTCACCGGGATTTTCGCACGAGACCCCGGATCGCGAGCCgctg < 2:631005/76‑1 (MQ=255)
ctcccGGTTCGATACGTTTATCGTTGGTTTTCACCGGGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCttt < 2:621044/73‑1 (MQ=255)
tACCGGTTCGATACGTTCATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTa < 1:134156/76‑1 (MQ=255)
|
CCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTA > REL606/3251958‑3252092
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
