Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A31 F1 I1 R1
|
21 |
37.9 |
2424921 |
96.5% |
2340048 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,465,213 |
A→C |
L19R (CTG→CGG) |
ompR ← |
osmolarity response regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,465,213 | 0 | A | C | 100.0%
| 168.4
/ NA
| 40 | L19R (CTG→CGG) | ompR | osmolarity response regulator |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (27/13); total (27/13) |
AGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCAAA > REL606/3465141‑3465284
|
agGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCcgcg < 1:1016784/76‑1 (MQ=255)
agGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCcgcg > 2:500535/1‑76 (MQ=255)
cGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGcgcac > 2:873742/1‑76 (MQ=255)
cGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGcgcac > 2:632261/1‑76 (MQ=255)
cGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGcgcac < 2:686485/76‑1 (MQ=255)
gATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGcgcacg > 1:489394/1‑76 (MQ=255)
ccATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAg > 2:525405/1‑76 (MQ=255)
ccATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAg < 1:386298/76‑1 (MQ=255)
aTCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATATCCTTCCAGCCGCGCACGCAGgc < 1:8332/76‑1 (MQ=255)
tCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGgcg > 2:804168/1‑76 (MQ=255)
cTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGgcgc > 1:949751/1‑76 (MQ=255)
cTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCGTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGgcgc < 1:170401/76‑1 (MQ=255)
tGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATgtcg > 2:31698/1‑76 (MQ=255)
cATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATgtcgtc > 2:726939/1‑76 (MQ=255)
aTTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCa < 2:614880/76‑1 (MQ=255)
aGCGACGCTTCGAACCTGGAAGCCTTGTTCGCTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCg < 2:198635/76‑1 (MQ=255)
gACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGacc > 2:869315/1‑76 (MQ=255)
cTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGAccacca < 1:607027/76‑1 (MQ=255)
cTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGAccacca > 1:499875/1‑76 (MQ=255)
cTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGAccacca > 2:465810/1‑76 (MQ=255)
ttCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAg > 1:941829/1‑76 (MQ=255)
ttCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAg > 1:401908/1‑76 (MQ=255)
ttCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAg > 2:458879/1‑76 (MQ=255)
gAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAat < 2:544343/76‑1 (MQ=255)
ttGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTg > 1:797021/2‑76 (MQ=255)
cTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTg > 1:797035/1‑76 (MQ=255)
cTGGAAGCCTTGTTCGGAGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTg > 2:84682/1‑76 (MQ=255)
tGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGt > 2:224533/1‑76 (MQ=255)
gAAGCCTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAg < 1:449931/76‑1 (MQ=255)
ccTTGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGNCATCGACCACCAGAATATTGTAGTtct < 1:1208031/76‑1 (MQ=255)
tGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCtt < 1:191571/76‑1 (MQ=255)
tGTTCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCtt < 1:520889/76‑1 (MQ=255)
gTTCGGTGAGATAACGTTCCAGCCGCGCACCCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTCTTCTTg > 1:1100993/1‑76 (MQ=255)
ttCGGTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGc > 1:376839/1‑76 (MQ=255)
gTGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCAttg > 2:225440/1‑76 (MQ=255)
tGAGATAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCAttgt > 2:608382/1‑76 (MQ=255)
agaTAACGTTCCAGCCGCGCACGCAGGCGCATGTCGTCAACGACCACCAGAATATTGTAGTTCTCTTGCAttgttt < 2:203143/76‑1 (MQ=255)
aaCGTTCCAGCCGCGCACGCAGGCGCATGTCGTNATCGACCACCAGAATATTGTAGNTCTCTTGCCTNGTTTGTAc > 2:1011022/1‑76 (MQ=255)
aaCGTTCCAGCCGCGCACGCAGGCGCATGTCGTNATCGACCACCAGAATATTGTAGNTCTCTTGCCTNGTTTGTAc > 2:1011008/1‑76 (MQ=255)
aaCGTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTAc > 1:735762/1‑76 (MQ=255)
gTTCCAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTcc > 1:312283/1‑76 (MQ=255)
ccAGCCGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCaa > 2:758680/1‑76 (MQ=255)
cagccgCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCaaa > 2:713960/1‑76 (MQ=255)
cagccgCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCaaa > 2:992687/1‑76 (MQ=255)
|
AGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGTTCTCTTGCATTGTTTGTACTCCCAAA > REL606/3465141‑3465284
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
