Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A31 F1 I1 R1
|
21 |
37.9 |
2424921 |
96.5% |
2340048 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,498,010 |
G→A |
S9L (TCG→TTG) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,498,010 | 0 | G | A | 100.0%
| 132.1
/ NA
| 34 | S9L (TCG→TTG) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/24); total (10/24) |
GGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTC > REL606/3497938‑3498077
|
gggTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATg < 1:886991/76‑1 (MQ=255)
ggTCCTTTCCAATCGTATACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGa < 1:128617/76‑1 (MQ=255)
ccTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAata > 2:963842/1‑76 (MQ=255)
ccTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAata > 1:348343/1‑76 (MQ=255)
cTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAatat < 1:1161554/76‑1 (MQ=255)
cTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAatat < 2:132032/76‑1 (MQ=255)
ttCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGt > 2:1162261/1‑76 (MQ=255)
cAATCAAAAGCATGNGCTTGTAACGGATAGAGTGCTTAAGCGCTCATACGCTAAGCGTGGGCGATGAATATGTaan < 2:171007/76‑2 (MQ=255)
aaTCGTAAGCATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAAc < 1:1082772/76‑1 (MQ=255)
cGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGa < 2:733791/76‑1 (MQ=255)
gTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAg > 2:560243/1‑76 (MQ=255)
aaCATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCAt < 1:174713/76‑1 (MQ=255)
gCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCAtata < 1:618432/76‑1 (MQ=255)
cTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAg < 2:517832/76‑1 (MQ=255)
cTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAg > 1:974734/1‑76 (MQ=255)
aaGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGtt > 2:1161008/1‑76 (MQ=255)
aGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGttt < 2:858135/76‑1 (MQ=255)
cGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTcc > 1:552397/1‑76 (MQ=255)
aTAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTg > 2:894661/1‑76 (MQ=255)
tAGAGTGCTTAAGNGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGa < 2:1110278/76‑1 (MQ=255)
agagTGCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGaa > 1:558070/1‑76 (MQ=255)
gCTTAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATGGGCGTTTCCTGAAAACTa < 1:90134/76‑1 (MQ=255)
tAAGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTAttt < 2:1867/76‑1 (MQ=255)
aaGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTc < 1:1114194/76‑1 (MQ=255)
aGGGCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa < 2:123994/76‑1 (MQ=255)
aGGGCTTCTACGCNAAGCGTGGGCAATGAATATGTAAACGGAGCAATCATATAGGCGTTTCCTGAAAACTATTTCa < 2:1107883/76‑1 (MQ=255)
gggCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCaa < 2:481956/76‑1 (MQ=255)
ggCTTCTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg < 2:350591/76‑1 (MQ=255)
cTACGCTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATa < 1:963843/76‑1 (MQ=255)
aCGNTAAGCGTGGGCAATGAATATGTAAACGGAGCATTCATATNGGCGTTTCTTGAAAACTATTTCAAGCGATAGt < 2:1128416/76‑1 (MQ=255)
aaGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGc > 1:659218/1‑76 (MQ=255)
aGCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt < 2:372501/76‑1 (MQ=255)
gCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATATTAAAGCTc < 2:1170334/76‑1 (MQ=255)
gCGTGGGCAATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTc < 2:1170336/76‑1 (MQ=255)
gCGTGGGCAATGAAGATGTAATCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTc < 2:181296/76‑1 (MQ=255)
|
GGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGGGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTC > REL606/3497938‑3498077
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
