Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A31 F1 I1 R1	21	37.9	2424921	96.5%	2340048	76.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,184,059	G→T	A385A (GCG→GCT)	zraR →	fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,184,059	0	G	T	100.0%	120.6 / NA	34	A385A (GCG→GCT)	zraR	fused DNA‑binding response regulator in two‑component regulatory system with ZraS: response regulator/sigma54 interaction protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/17); total (17/17)

GAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTG  >  REL606/4183988‑4184131

gAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTg                                                                      <  2:1192534/76‑1 (MQ=255)

   aaggcgGTGGAAGGGGCAGGGGTGCGGCTGAGGGGGGAATATATCTCCGAACGCGAGCTGCCGCTGGCTATTGCCa                                                                   <  2:791276/73‑1 (MQ=255)

   aaCGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCa                                                                   <  2:791293/76‑1 (MQ=255)

       cgGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTAc                                                               >  1:482617/1‑76 (MQ=255)

          tGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGcc                                                            <  1:649930/76‑1 (MQ=255)

            gAACTGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGa                                                          <  2:1205793/76‑1 (MQ=255)

            gAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGa                                                          >  1:324413/1‑76 (MQ=255)

               cGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATcc                                                       <  1:319979/76‑1 (MQ=255)

                gggCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATccc                                                      <  2:324288/76‑1 (MQ=255)

                         tgctgcTGACCGGGAAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACa                                             <  1:662682/76‑1 (MQ=255)

                          gctgctGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACaa                                            >  2:1079139/1‑76 (MQ=255)

                          gctgctGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTCGTACaa                                            <  1:74041/76‑1 (MQ=255)

                            tgctgACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAg                                          <  2:893712/76‑1 (MQ=255)

                               tgACCGGGGTATATATTTCCGAANGCGAGNTGCCGATGGCTATTNCCTGTAGGCCGATCCCACTGGGACAAAGTCa                                       >  2:152784/1‑76 (MQ=255)

                               tgACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCa                                       >  1:489565/1‑76 (MQ=255)

                                gACCGGGGAATATATTTCCGAAGGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAg                                      >  1:5101/1‑76 (MQ=255)

                                  ccGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGa                                    >  2:480716/1‑76 (MQ=255)

                                     gggAATATNTGCCCGAACGNGGGCTGCCGCTGGCTATTGCCANTACGCCGGTCCCGCTGGGACAAAGTCAGGatat                                 <  2:1019389/76‑1 (MQ=255)

                                      ggAATATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATAtt                                <  1:176762/76‑1 (MQ=255)

                                        aaTATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCa                              >  2:523211/1‑76 (MQ=255)

                                        aaTATATTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCa                              >  1:750993/1‑76 (MQ=255)

                                          tatatTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGc                            >  2:961871/1‑76 (MQ=255)

                                           atatTTCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCACGCCGGGGCAAAGCGAGGATTTTAAgac                           >  2:1076845/1‑74 (MQ=255)

                                               ttCCGAACGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCNGCNGGGACAAAGTCAGGNTATTCAGCCGTtg                       >  2:1087693/1‑76 (MQ=255)

                                               tcccGACCGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTtg                       <  2:638019/74‑1 (MQ=255)

                                                    aaCGCGAGCTGCCGCTGGCTATTGCCAGTACGCCGAGCCCGCTGGGACAAAGTCAGGNTATTCAGCCGTTGgtgga                  <  1:1155718/76‑1 (MQ=255)

                                                      ggcgAGCTGCCGCTGGATGTTGTCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGgtggaag                <  2:46234/75‑1 (MQ=255)

                                                      cgggAGCTGCCGCTGGCTATTGACAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGgtggaag                <  2:46244/73‑1 (MQ=255)

                                                      cgcgAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGgtggaag                >  2:829993/1‑76 (MQ=255)

                                                         gAGCTGCCGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGgtggcagagg             >  2:451128/1‑76 (MQ=255)

                                                               ccGCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAag       <  2:885991/76‑1 (MQ=255)

                                                                 gCTGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAagag     >  1:926503/1‑76 (MQ=255)

                                                                  cTGGCTATTCCCAGTACGCCGATCCCGCTGGGATAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAgg    >  2:1035263/1‑76 (MQ=255)

                                                                   tGGCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGt   <  2:575473/76‑1 (MQ=255)

                                                                    ggCTATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTg  >  2:960439/1‑76 (MQ=255)

GAAAACGCGGTGGAACGGGCAGTGGTGCTGCTGACCGGGGAATATATTTCCGAACGCGAGCTGCCGCTGGCGATTGCCAGTACGCCGATCCCGCTGGGACAAAGTCAGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTG  >  REL606/4183988‑4184131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A