Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A31 F1 I1 R1
|
21 |
37.9 |
2424921 |
96.5% |
2340048 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,194,135 |
G→T |
S137S (TCG→TCT) |
metA → |
homoserine O‑succinyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,194,135 | 0 | G | T | 98.0%
| 178.1
/ NA
| 50 | S137S (TCG→TCT) | metA | homoserine O‑succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (32/17); minor base C (1/0); total (33/17) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
GGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCAC > REL606/4194063‑4194206
|
ggANTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGNTGGAGTGGTCGAAAGATCACGTCACCTCTACg < 2:1132661/76‑1 (MQ=255)
ggAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACg > 2:637339/1‑76 (MQ=255)
ggAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACg < 1:936140/76‑1 (MQ=255)
ggAGATAAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACg > 1:69498/1‑76 (MQ=255)
gTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTg > 1:311946/1‑76 (MQ=255)
tttAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGt > 2:210767/1‑76 (MQ=255)
tAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGttt > 1:519102/1‑76 (MQ=255)
atgatgTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGt < 2:703677/76‑1 (MQ=255)
atgTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTctg < 2:852735/76‑1 (MQ=255)
atgTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTctg > 2:450259/1‑76 (MQ=255)
cGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTgg > 1:931592/1‑76 (MQ=255)
gCTTACTGGCCGCANATCAAACAGGTGCTGGAGTNGTCGATAGNTCACGTCACCTCTACGCTGATTGTCTGCTggg < 2:1003248/76‑1 (MQ=255)
cTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGc > 1:338124/1‑76 (MQ=255)
cTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGc < 1:422388/76‑1 (MQ=255)
ttACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACTCTGTTTGTCTGCTGGGCg > 1:1141473/1‑76 (MQ=255)
ttACTGGCCGCAGATCAAACAGGTGCTGGAGGGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTTGTGGGCg > 2:976299/1‑76 (MQ=255)
tACTGGCCGCAGATCAACCAGGCGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCgg > 2:184702/1‑76 (MQ=255)
tACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCgg > 2:61427/1‑76 (MQ=255)
aCTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGt > 1:460137/1‑76 (MQ=255)
tGGCCGCAGATCAAACGGGTGCTGGAGTGGTCGAAAGATCTCGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTAc < 1:606710/76‑1 (MQ=255)
tGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTAc > 1:726560/1‑76 (MQ=255)
tGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTAc > 2:1177076/1‑76 (MQ=255)
ggCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACa > 1:1027002/1‑76 (MQ=255)
ggCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACa > 1:858594/1‑76 (MQ=255)
ggCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGAGGTACa > 2:1177872/1‑76 (MQ=255)
gCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAg > 2:776870/1‑76 (MQ=255)
gCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGcc < 2:604921/76‑1 (MQ=255)
gCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTCTGTCTGATGGGCGGTACAGGcc > 2:42398/1‑76 (MQ=255)
aTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCgcgc > 1:1161437/1‑76 (MQ=255)
cAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTc > 2:618323/1‑76 (MQ=255)
aaaCAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCa < 1:640348/76‑1 (MQ=255)
aaaCAGGTGCTGGAGTGGTCGAAAGATCACGTCACCGCTACGCTGTTTGTCTGCTGGGCGGTACAGGNCGCGCTCa > 1:1059816/1‑76 (MQ=255)
aCAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAat > 1:859005/1‑76 (MQ=255)
aGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAatat < 2:53225/76‑1 (MQ=255)
gTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCGGCGCTCAATATcc < 1:79004/76‑1 (MQ=255)
gTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATcc > 1:577393/1‑76 (MQ=255)
tGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCct > 2:495702/1‑76 (MQ=255)
gCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCctc > 2:461166/1‑76 (MQ=255)
tGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTa > 1:1022130/1‑76 (MQ=255)
nggAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCNCTCAATATCCTCTa > 2:163603/2‑76 (MQ=255)
ggAGTGGTCGAAACATCACGTCAGCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTAc < 1:1041307/76‑1 (MQ=255)
gAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACg < 1:216040/76‑1 (MQ=255)
tCGAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTc < 2:581298/76‑1 (MQ=255)
gAAAGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCt < 1:100497/76‑1 (MQ=255)
aaaGATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTa > 2:894731/1‑76 (MQ=255)
aaaGATCACGTCACCTCCACGCAGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCCATATCCCCTACGGCATTCCTa > 2:176522/1‑76 (MQ=255)
gATCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGc < 1:871544/76‑1 (MQ=255)
gATCACGTCACCTCTACGCTGTATGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGc < 2:40461/76‑1 (MQ=255)
aTCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCa > 1:1166632/1‑76 (MQ=255)
aTCACGTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCa > 2:1174908/1‑76 (MQ=255)
gTCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTc > 2:370870/1‑76 (MQ=255)
tCACCTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCg > 2:128366/1‑76 (MQ=255)
ccTCTACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCAc < 1:1116929/76‑1 (MQ=255)
|
GGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCAC > REL606/4194063‑4194206
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
