Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A31 F1 I1 R1
|
21 |
37.9 |
2424921 |
96.5% |
2340048 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,194,420 |
G→T |
V232V (GTG→GTT) |
metA → |
homoserine O‑succinyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,194,420 | 0 | G | T | 100.0%
| 199.6
/ NA
| 52 | V232V (GTG→GTT) | metA | homoserine O‑succinyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (26/26); total (26/26) |
TGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAC > REL606/4194347‑4194492
|
tGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTAc < 1:894732/76‑1 (MQ=255)
ggAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACg < 1:370871/76‑1 (MQ=255)
aTTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGcc > 2:1182272/1‑76 (MQ=255)
tCTGGCAGAGTCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCAt > 1:1120035/1‑76 (MQ=255)
gcaggCAGAGACGNAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCAt < 1:160576/73‑1 (MQ=255)
cTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATc < 2:1067543/76‑1 (MQ=255)
tGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATcc > 1:791206/1‑76 (MQ=255)
tGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATcc < 1:547368/76‑1 (MQ=255)
cAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGa < 1:1115724/76‑1 (MQ=255)
cAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATACCGa < 1:73418/76‑1 (MQ=255)
agagACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGaa > 1:676500/1‑76 (MQ=255)
gagaCGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAat > 2:86749/1‑76 (MQ=255)
agaCGGAAGAAGGTGATGCATATCTGTTTGCCANTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAata > 2:1020586/1‑76 (MQ=255)
ggAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATatgat < 1:912268/76‑1 (MQ=255)
gaggaagGGGATGCATATCTGTTTGCCAGT‑AAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATgc < 2:618917/73‑1 (MQ=255)
gaagaaGGGGATGCATATCTCTNNGCTAGTAAAGNNTAGCGCATTGCCTTGGTTACGGGCCATCCCGAATatgatg < 1:1093043/76‑1 (MQ=255)
aagaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATgc < 1:763073/76‑1 (MQ=255)
agaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATgcg > 1:382223/1‑76 (MQ=255)
gaagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATgcgc < 1:495703/76‑1 (MQ=255)
aagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCa < 1:944064/76‑1 (MQ=255)
aagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCa < 1:1174909/76‑1 (MQ=255)
aagGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCa > 1:652491/1‑76 (MQ=255)
agGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCaa < 2:989999/76‑1 (MQ=255)
ggggATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCaaa < 2:65858/76‑1 (MQ=255)
gCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTgg > 1:783547/1‑76 (MQ=255)
atatCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGgcg > 1:781954/1‑76 (MQ=255)
atatCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGgcg < 1:461167/76‑1 (MQ=255)
cTGTGTTCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCTAATATGATGCGCAAACGCTGGCGCAgg < 1:1199284/76‑1 (MQ=255)
tttGCCAGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAt > 1:1170948/1‑76 (MQ=255)
aGTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTcc < 2:219897/76‑1 (MQ=255)
gTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAAtttttacc > 1:943793/1‑73 (MQ=255)
gTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcg > 1:943825/1‑76 (MQ=255)
gTAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcg > 2:574878/1‑76 (MQ=255)
tAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc > 1:836941/1‑76 (MQ=255)
tAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc > 2:741384/1‑76 (MQ=255)
tAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc > 2:671833/1‑76 (MQ=255)
tAAAGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgc > 2:540644/1‑76 (MQ=255)
aaaGATAAGNGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgcg > 1:30353/1‑76 (MQ=255)
aaaGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgcg > 1:643776/1‑76 (MQ=255)
aaaGATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCcgcg > 2:722225/1‑76 (MQ=255)
gATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtg < 1:722848/76‑1 (MQ=255)
gATAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtg > 2:916180/1‑76 (MQ=255)
tAAGCGTATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg > 1:71066/1‑76 (MQ=255)
tAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgtg < 2:237741/76‑1 (MQ=255)
tAAGCGCATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGAtgta < 2:1077426/76‑2 (MQ=255)
gcgcATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGaa < 1:695987/76‑1 (MQ=255)
gcATTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGc < 1:859433/76‑1 (MQ=255)
aTTGCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCg < 2:976622/76‑1 (MQ=255)
gCCTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCCCGATGTGGAAGCCGGAc > 1:126361/1‑76 (MQ=255)
ccTTTGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACt < 1:833921/76‑1 (MQ=255)
tttGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAg > 2:266036/1‑76 (MQ=255)
ttGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGa < 1:444378/76‑1 (MQ=255)
ttGTTACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGa > 1:487892/1‑76 (MQ=255)
tgttACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAc > 2:1079715/1‑76 (MQ=255)
|
TGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGAC > REL606/4194347‑4194492
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
