Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A31 F1 I1 R1
|
21 |
37.9 |
2424921 |
96.5% |
2340048 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,421,946 |
T→A |
D66E (GAT→GAA) |
cysQ → |
PAPS (adenosine 3'‑phosphate 5'‑phosphosulfate) 3'(2'),5'‑bisphosphate nucleotidase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,421,946 | 0 | T | A | 100.0%
| 117.4
/ NA
| 33 | D66E (GAT→GAA) | cysQ | PAPS (adenosine 3'‑phosphate 5'‑phosphosulfate) 3'(2'),5'‑bisphosphate nucleotidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (14/19); total (14/19) |
TGCCGCTCACACCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGATCCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGATGGTACTAAAGA > REL606/4421874‑4422017
|
tGCCGCTCACACCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAacct < 2:500164/76‑1 (MQ=255)
ccGCTCACACCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAacctcc < 1:536218/76‑1 (MQ=255)
gCTCACTCCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCg < 2:1176303/76‑1 (MQ=255)
cacCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTgg > 2:402305/1‑76 (MQ=255)
acCGTTATNATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTggg < 1:1064985/76‑1 (MQ=255)
acCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTggg < 2:1069253/76‑1 (MQ=255)
acCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTggg > 1:946169/1‑76 (MQ=255)
acCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTggg > 2:834624/1‑76 (MQ=255)
gTTATCATGGACGGTTTACGTACGCTCACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAg > 2:580469/1‑76 (MQ=255)
aTCATGGACGGTTTACGTACGCTGACACCGGANATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAANGcc > 2:1133585/1‑76 (MQ=255)
tcctGGACGGATTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGACGAAGAACCTCCCGGTTGGACAGTCCg > 2:524716/4‑76 (MQ=255)
aTGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTc < 2:907190/76‑1 (MQ=255)
tGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCa < 2:810668/76‑1 (MQ=255)
gACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGc > 2:1172523/1‑76 (MQ=255)
cGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCAc > 2:854591/1‑76 (MQ=255)
ttACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGc < 2:490039/76‑1 (MQ=255)
ttACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGc < 2:490056/76‑1 (MQ=255)
cTGACACCNGATATTCCGGNCCTTTCTGAAGAAGAACCTCCCNGTTGGGAAGTCCGTCAGCACTGGCAGCGTTAct < 2:1008463/76‑1 (MQ=255)
cTGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTAct < 2:541885/76‑1 (MQ=255)
tGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTActg > 1:319772/1‑76 (MQ=255)
tGACACCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGGTActg > 2:930125/1‑76 (MQ=255)
acCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTActggctg > 2:976606/1‑76 (MQ=255)
acCGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTActggctg > 2:53904/1‑76 (MQ=255)
cGGATATTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCNTTACTGGCTGGt < 1:13465/76‑1 (MQ=255)
tatTCCGGTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGGAGAc > 2:576910/1‑76 (MQ=255)
tCCGGTCCTTTCTGAAGAAGAACTTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCg < 1:1043880/76‑1 (MQ=255)
ggTCCTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTg < 2:1066087/76‑1 (MQ=255)
ccTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGAt < 2:642927/76‑1 (MQ=255)
cTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGATg < 2:27114/76‑1 (MQ=255)
cTTTCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGATg < 2:726200/76‑1 (MQ=255)
tttCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGGAGACCCGCTGGGTgg > 2:447120/1‑76 (MQ=255)
tCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGATGGTa < 2:428138/76‑1 (MQ=255)
tCTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGATGGTa < 1:854592/76‑1 (MQ=255)
cTGAAGAAGAACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGATGGTAc < 1:376586/76‑1 (MQ=255)
agaagaACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGATGGTACTaaa < 1:516879/76‑1 (MQ=255)
aagaACCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGGAGACCCGCTGGGTGGTACTAAaga > 2:9170/1‑76 (MQ=255)
|
TGCCGCTCACACCGTTATCATGGACGGTTTACGTACGCTGACACCGGATATTCCGGTCCTTTCTGAAGAAGATCCTCCCGGTTGGGAAGTCCGTCAGCACTGGCAGCGTTACTGGCTGGTAGACCCGCTGGATGGTACTAAAGA > REL606/4421874‑4422017
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
