Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I1 R1
|
15 |
61.9 |
4096487 |
94.5% |
3871180 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,438,531 |
T→G |
D110A (GAC→GCC) |
fbp ← |
fructose‑1,6‑bisphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,438,531 | 0 | T | G | 97.8%
| 166.4
/ ‑6.3
| 45 | D110A (GAC→GCC) | fbp | fructose‑1,6‑bisphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (19/25); minor base A (0/1); total (19/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAA > REL606/4438458‑4438603
|
aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc > 1:709995/1‑76 (MQ=255)
aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc > 2:572690/1‑76 (MQ=255)
aCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCa > 2:1239537/1‑76 (MQ=255)
cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGCNGCCATCa < 2:1648258/76‑1 (MQ=255)
tAGATGGAGAAAATGGTNACGACAGAGACGTTAACATCGATGTTGGACGAGCTATCCAGGGGGGCCGTCAGcacca > 1:258796/1‑76 (MQ=255)
tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacca > 2:1074359/1‑76 (MQ=255)
tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacca > 2:637998/1‑76 (MQ=255)
gagaAAATGGTNCCNACAGAGACGTTCACATCGATGTTGGACGAGCCATCCAGGGGGATCATCAGCTCCCCGTAtt < 2:325070/76‑1 (MQ=255)
gaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCGNGTAtttt < 2:1652830/76‑1 (MQ=255)
ggTACCGACAGCGACCTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgtgt > 1:61572/1‑76 (MQ=255)
cccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca < 2:1294318/75‑1 (MQ=255)
ccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcac < 2:956105/76‑1 (MQ=255)
cGACAGAGACGTTAACATCGTTGTTGGACGAGCCATCCAGGGGGGCCATCAGCGCCACGTATTTTGCGTGTTcaca > 2:167904/1‑76 (MQ=255)
cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca < 2:495763/76‑1 (MQ=255)
gACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAg < 2:1600611/76‑1 (MQ=255)
gACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGAGCCATCAGCACCACGTATTTTCCGTGTTCagag < 1:1867716/76‑1 (MQ=255)
aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc > 1:1103327/1‑76 (MQ=255)
aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc > 2:394534/1‑76 (MQ=255)
cAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGcc > 2:1596101/1‑76 (MQ=255)
agagACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCt > 1:964634/1‑76 (MQ=255)
agagACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCt > 2:1022367/1‑76 (MQ=255)
agagACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCt > 2:746774/1‑76 (MQ=255)
gagaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCtt < 1:1264321/76‑1 (MQ=255)
aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 1:862572/76‑1 (MQ=255)
aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 1:1150875/76‑1 (MQ=255)
aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGCGTTCACAGCCTTCaa > 1:1687308/1‑76 (MQ=255)
aTCGATGTTGGANGANCCATCCAGGGGGGCCATCNGCACCATGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa < 1:1669452/76‑1 (MQ=255)
cGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAt < 1:746775/76‑1 (MQ=255)
aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtct > 2:1481302/1‑76 (MQ=255)
aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGACTTCAAAGACGACAAtct < 1:1925088/76‑1 (MQ=255)
ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt < 2:556139/76‑1 (MQ=255)
ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt < 1:1142859/76‑1 (MQ=255)
ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt < 1:1339249/76‑1 (MQ=255)
ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGAGTTCACAGCCTTCAACGACGACAATCTCAt > 1:43385/1‑76 (MQ=255)
tGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtc > 2:677952/1‑76 (MQ=255)
gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt < 2:1584976/76‑1 (MQ=255)
gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt > 2:1071652/1‑76 (MQ=255)
gAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt > 2:476181/1‑76 (MQ=255)
ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 2:1450621/76‑1 (MQ=255)
ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 2:1450603/76‑1 (MQ=255)
aTCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCa > 1:1937175/1‑76 (MQ=255)
ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga < 1:1503021/76‑1 (MQ=255)
ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga < 1:1476445/76‑1 (MQ=255)
cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag < 1:838573/76‑1 (MQ=255)
aGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAgg < 2:1299542/76‑1 (MQ=255)
ggggggCCATCAGCCCCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCGTCTTCTCCTTCAGAGGc < 1:168935/76‑1 (MQ=255)
ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:1703940/76‑1 (MQ=255)
ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:1425147/76‑1 (MQ=255)
ggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCaa > 2:980975/1‑76 (MQ=255)
ggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCaa > 2:629529/1‑76 (MQ=255)
ggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCaa > 2:495473/1‑76 (MQ=255)
|
AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCAA > REL606/4438458‑4438603
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
