breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 474,424 A→T D294E (GAT→GAA ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
JC JC 1,305,435 IS150 (–) +3 bp coding (803‑805/1461 nt) cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 1,886,183 A→G intergenic (‑13/+656) yobF ← / ← yebO hypothetical protein/hypothetical protein
JC 1,932,316 (GCA)3→2 coding (105‑107/972 nt) yecP → predicted S‑adenosyl‑L‑methionine‑dependent methyltransferase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,145,869 G→T E2D (GAG→GAT rpoD → RNA polymerase sigma factor
RA 3,328,962 C→T R6H (CGT→CAT)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 4,166,492 A→C Y511S (TAC→TCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA 4,438,531 T→G D110A (GAC→GCC)  fbp ← fructose‑1,6‑bisphosphatase
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546989–547700 593401 45702–46413 20 [17] [17] 18 [insB‑6]–[fepA] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593566 619842–619116 25551–26277 19 [17] [17] 18 [fepA]–insA‑8 [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8
* * ÷ REL606 3638858–3638920 3639041–3638990 71–184 18 [17] [15] 18 dppA/proK dipeptide transporter/tRNA‑Pro