Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A92 F1 I1 R1
|
15 |
58.2 |
3997918 |
96.1% |
3841999 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,438,531 |
T→G |
D110A (GAC→GCC) |
fbp ← |
fructose‑1,6‑bisphosphatase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,438,531 | 0 | T | G | 96.9%
| 390.2
/ ‑2.2
| 97 | D110A (GAC→GCC) | fbp | fructose‑1,6‑bisphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base G (39/55); total (40/57) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
Only 100 of 102 total aligned reads displayed.
AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCA > REL606/4438458‑4438602
|
aaCGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc < 1:150724/76‑1 (MQ=255)
aaCGCGGCGGCAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGcc < 2:1374842/76‑1 (MQ=255)
cgcgGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCAt < 2:403715/76‑1 (MQ=255)
cggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCa < 2:1863088/76‑1 (MQ=255)
ggcggTAGATGGAGAAAATGGTACCGACAGAGGCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg < 1:255615/76‑1 (MQ=255)
ggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg > 1:1116344/1‑76 (MQ=255)
ggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg > 1:514650/1‑76 (MQ=255)
ggcggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAg < 2:434777/76‑1 (MQ=255)
cggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcg < 1:244545/76‑2 (MQ=255)
cggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGca > 1:275581/1‑76 (MQ=255)
cggTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGca < 2:669767/76‑1 (MQ=255)
gTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacc < 2:1629183/76‑1 (MQ=255)
gTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacc < 1:1558315/76‑1 (MQ=255)
tAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcacca > 1:1653359/1‑76 (MQ=255)
aGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGcaccac < 1:1268662/76‑1 (MQ=255)
gATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACg > 2:95378/1‑76 (MQ=255)
aTGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGt > 2:1107793/1‑76 (MQ=255)
ggAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAt > 2:1671344/1‑76 (MQ=255)
gaAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTAtttt < 2:593038/76‑1 (MQ=255)
aaaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTg > 2:279666/1‑76 (MQ=255)
aaaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTg < 2:1978759/76‑1 (MQ=255)
aaaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTg < 2:1177261/76‑1 (MQ=255)
aaaTGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGc < 2:222472/76‑1 (MQ=255)
aaaTGGTACCGACAGAGACGTTAACATCGAGGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGc < 2:142606/76‑1 (MQ=255)
agggTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgt < 2:1731238/74‑1 (MQ=255)
tGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgtg > 1:1659670/1‑76 (MQ=255)
tGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCgtg < 2:452386/76‑1 (MQ=255)
gTACGGACAGAGACTGTAACATCGATGGTGGTCGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGtt < 2:329068/76‑1 (MQ=255)
tACCGACAGAGACGTTAACATCGATGTTGGACGAGTCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTc > 2:1800431/1‑76 (MQ=255)
tACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTc > 2:1086926/1‑76 (MQ=255)
tACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTc > 2:949354/1‑76 (MQ=255)
aCCGACATGGACGTTAACACCGGGGTGGAAAGAGACATCCAGGGGGGCCATCAGGACCACGTATTTTGCGTGTTca < 2:761789/76‑1 (MQ=255)
aCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca > 2:712076/1‑76 (MQ=255)
aCCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTca > 1:825582/1‑76 (MQ=255)
ccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcac < 2:441090/76‑1 (MQ=255)
ccGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcac < 2:964109/76‑1 (MQ=255)
cGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTcaca < 1:806508/76‑1 (MQ=255)
gACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAg < 1:632788/76‑1 (MQ=255)
aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc < 1:1629222/76‑1 (MQ=255)
aCAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGc > 2:74115/1‑76 (MQ=255)
gagaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCtt < 2:1290026/76‑1 (MQ=255)
agaAGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTc < 2:606104/76‑1 (MQ=255)
gaCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCa < 1:682363/76‑1 (MQ=255)
aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 2:1156750/76‑1 (MQ=255)
aCGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaa < 1:592827/76‑1 (MQ=255)
cGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa < 1:905924/76‑1 (MQ=255)
cGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa > 1:418530/1‑76 (MQ=255)
cGTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCaaa > 1:131935/1‑76 (MQ=255)
gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg < 2:224244/76‑1 (MQ=255)
gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg < 2:1870605/76‑1 (MQ=255)
gTTAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAg < 1:711026/76‑1 (MQ=255)
ttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga < 2:1343457/76‑1 (MQ=255)
ttAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga > 2:1762236/1‑76 (MQ=255)
tgaaCGTCGATGTTGGCCGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAga < 2:348068/74‑1 (MQ=255)
tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac > 2:1083837/1‑76 (MQ=255)
tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac < 1:612725/76‑1 (MQ=255)
tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac > 1:1360895/1‑76 (MQ=255)
tAACATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgac < 1:799408/76‑1 (MQ=255)
aaCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg > 2:1977239/1‑76 (MQ=255)
aaCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacg > 1:17469/1‑76 (MQ=255)
aCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCACAgacga > 2:780729/1‑76 (MQ=255)
aCATCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAgacga < 2:660691/76‑1 (MQ=255)
cATCGATGTGGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAGAgacgac < 1:1277923/76‑1 (MQ=255)
aTCGATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACa < 2:1351692/76‑1 (MQ=255)
gATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtc > 2:553763/1‑76 (MQ=255)
gATGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtc < 1:1663333/76‑1 (MQ=255)
aTGTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAAtct > 2:902651/1‑76 (MQ=255)
gTTGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACCGCCTCCCAAGACGACAATCTCa > 2:520190/1‑76 (MQ=255)
ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt > 1:1055651/1‑76 (MQ=255)
ttGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAt > 1:1366773/1‑76 (MQ=255)
tGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtc > 1:1306225/1‑76 (MQ=255)
tGGACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtc > 1:135897/1‑76 (MQ=255)
gggcGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtct < 2:1055651/73‑1 (MQ=255)
gACGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtctt < 1:73389/76‑1 (MQ=255)
aCGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttc < 2:1175315/76‑1 (MQ=255)
cGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttct < 1:1012836/76‑1 (MQ=255)
cGAGCCATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttct > 2:1113849/1‑76 (MQ=255)
gAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttctt < 1:1740963/76‑1 (MQ=255)
gCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCAtcttcttct > 1:1236259/1‑76 (MQ=255)
ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 2:321119/76‑1 (MQ=255)
ccATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATcttcttctt < 1:149936/76‑1 (MQ=255)
cATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCttcttcttc < 1:56119/76‑1 (MQ=255)
cATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCttcttcttc < 1:1991394/76‑1 (MQ=255)
cATCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCttcttcttc > 1:854419/1‑76 (MQ=255)
cATCCAGAGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCttcttcttc > 1:1275096/1‑76 (MQ=255)
aTCCAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCa < 2:1891977/76‑1 (MQ=255)
ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga > 1:1857915/1‑76 (MQ=255)
ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga < 2:1439182/76‑1 (MQ=255)
ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga > 2:672031/1‑76 (MQ=255)
ccAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCaga > 2:681031/1‑76 (MQ=255)
cAGGGGGGCCATCAGCGCCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag < 2:846561/76‑1 (MQ=255)
cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCatag > 1:1626055/1‑76 (MQ=255)
cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag < 2:626014/76‑1 (MQ=255)
cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag > 2:1242590/1‑76 (MQ=255)
cAGGGGGGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCagag > 2:1506894/1‑76 (MQ=255)
ggggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGc < 2:1599578/76‑1 (MQ=255)
ggggggCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGc < 1:1800431/76‑1 (MQ=255)
ggggTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:593342/76‑1 (MQ=255)
ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:412593/76‑1 (MQ=255)
ggggGCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCa < 1:327113/76‑1 (MQ=255)
|
AACGCGGCGGTAGATGGAGAAAATGGTACCGACAGAGACGTTAACATCGATGTTGGACGAGCCATCCAGGGGGTCCATCAGCACCACGTATTTTGCGTGTTCACAGCCTTCAAAGACGACAATCTCATCTTCTTCTTCAGAGGCA > REL606/4438458‑4438602
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
