Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A39 F1 I1 R1
|
15 |
59.3 |
3695894 |
97.1% |
3588713 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,465,186 |
C→T |
G28D (GGC→GAC) |
ompR ← |
osmolarity response regulator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,465,186 | 0 | C | T | 100.0%
| 220.6
/ NA
| 56 | G28D (GGC→GAC) | ompR | osmolarity response regulator |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (33/23); total (33/23) |
ATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGT > REL606/3465114‑3465258
|
aTAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCtt < 1:923534/76‑1 (MQ=255)
tAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTg > 2:1607613/1‑76 (MQ=255)
aaGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGt > 2:89426/1‑76 (MQ=255)
gATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTc < 2:1155258/76‑1 (MQ=255)
tGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCgg > 2:1588369/1‑76 (MQ=255)
ggAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGt > 2:987681/1‑76 (MQ=255)
ggAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGt > 2:464773/1‑76 (MQ=255)
ggAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGt > 1:283078/1‑76 (MQ=255)
aaaGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTga > 2:1001058/1‑76 (MQ=255)
aTTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATa > 2:182306/1‑76 (MQ=255)
ttCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATaa < 1:940319/76‑1 (MQ=255)
ttCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATaa > 1:702356/1‑76 (MQ=255)
aCGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGGGATAACGt > 2:908023/1‑76 (MQ=255)
gAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTc < 2:1670247/76‑1 (MQ=255)
cagcagGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCcagc > 2:883045/1‑76 (MQ=255)
cagGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCagcagc > 2:1512516/1‑76 (MQ=255)
agGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAgcg > 2:980816/1‑76 (MQ=255)
agGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAgcg < 1:376571/76‑1 (MQ=255)
agGCGATCCATCTGTTCTGCATTAGCGACACTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAgcg > 1:170546/1‑76 (MQ=255)
gCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGcgca > 1:88220/1‑76 (MQ=255)
cGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGcgcac > 1:450359/1‑76 (MQ=255)
gATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGcgcacg > 2:892835/1‑76 (MQ=255)
aTCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgc > 1:833149/1‑76 (MQ=255)
aTCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgc > 1:641638/1‑76 (MQ=255)
tCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgcg < 2:1739394/76‑1 (MQ=255)
tCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgcg < 2:16701/76‑1 (MQ=255)
tCTGTTCTGCATTAGCAACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGgcg > 2:803641/1‑76 (MQ=255)
tGTTCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCa > 1:1452029/1‑76 (MQ=255)
tCTGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgt > 1:978222/1‑76 (MQ=255)
tGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtcg < 1:187741/76‑1 (MQ=255)
tGCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtcg < 1:1454268/76‑1 (MQ=255)
gCATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtcgt > 2:458959/1‑76 (MQ=255)
cATTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATgtcgtc > 2:1732860/1‑76 (MQ=255)
aTTAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCa < 1:480029/76‑1 (MQ=255)
tAGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATc < 2:450359/76‑1 (MQ=255)
aGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCg < 1:1800490/76‑1 (MQ=255)
aGCGACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACATTCCAGCAGCGCACGCAGGCGCATGTCGTCATCg > 2:1351598/1‑76 (MQ=255)
gACGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGacc < 1:1607613/76‑1 (MQ=255)
cGCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGaccac < 2:1826554/76‑1 (MQ=255)
gCTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGaccacc > 1:6900/1‑76 (MQ=255)
cTTCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGAccacca > 2:1799088/1‑76 (MQ=255)
ttCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAg < 1:1063795/76‑1 (MQ=255)
tCGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGa > 2:630335/1‑76 (MQ=255)
cGAACCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGaa > 2:45544/1‑76 (MQ=255)
aaCCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAata < 2:457939/76‑1 (MQ=255)
aaCCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAata < 2:1697578/76‑1 (MQ=255)
aCCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAatat < 1:823103/76‑1 (MQ=255)
aCCTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAatat < 2:843668/76‑1 (MQ=255)
ccTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATAtt < 2:142636/76‑1 (MQ=255)
ccTGGAAGTCTTGTTCGGTGAGATAACGTACCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATAtt < 2:316634/76‑1 (MQ=255)
cTGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTg > 1:1809504/1‑76 (MQ=255)
tGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGt < 2:847062/76‑1 (MQ=255)
tGGAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGt > 2:115917/1‑76 (MQ=255)
gAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAg > 2:749456/1‑76 (MQ=255)
gAAGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAg < 1:1823644/76‑1 (MQ=255)
aaGTCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGt > 1:96159/1‑76 (MQ=255)
|
ATAAGATGGAAAGATTCACGAGTCAGCAGGCGATCCATCTGTTCTGCATTAGCGACGCTTCGAACCTGGAAGCCTTGTTCGGTGAGATAACGTTCCAGCAGCGCACGCAGGCGCATGTCGTCATCGACCACCAGAATATTGTAGT > REL606/3465114‑3465258
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
