Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A55 F1 I1 R1
|
18 |
62.6 |
3986840 |
96.5% |
3847300 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,252,012 |
G→A |
A250V (GCT→GTT) |
nusA ← |
transcription elongation factor NusA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,252,012 | 0 | G | A | 97.7%
| 160.8
/ ‑3.1
| 44 | A250V (GCT→GTT) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (22/21); total (22/22) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.86e-01 |
GACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCG > REL606/3251939‑3252084
|
gACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACa < 1:1360110/76‑1 (MQ=255)
atatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTa < 2:221203/76‑1 (MQ=255)
atatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTa < 2:523791/76‑1 (MQ=255)
cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCgg > 1:1756851/1‑76 (MQ=255)
aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGAt < 2:1776841/76‑1 (MQ=255)
tACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATc < 1:1401309/76‑1 (MQ=255)
aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCg < 1:1160488/76‑1 (MQ=255)
aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCg < 2:1832063/76‑1 (MQ=255)
aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCg > 2:1441360/1‑76 (MQ=255)
aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCg > 2:515734/1‑76 (MQ=255)
aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCg < 2:613514/76‑1 (MQ=255)
aCGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCg > 1:426005/1‑76 (MQ=255)
cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATa > 2:1356376/1‑76 (MQ=255)
cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATCCCTACGCAAACACCTACCGGATCGATCCg > 2:969126/1‑76 (MQ=255)
cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACg < 2:1570988/76‑1 (MQ=255)
aCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTa > 2:899767/1‑76 (MQ=255)
aCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTa > 2:354775/1‑76 (MQ=255)
cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTAt < 2:987226/76‑1 (MQ=255)
ccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATc < 2:434010/76‑1 (MQ=255)
cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCg > 1:700564/1‑76 (MQ=255)
aGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGt > 2:1646868/1‑76 (MQ=255)
tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTgg < 2:998990/76‑1 (MQ=255)
tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTgg > 2:1693729/1‑76 (MQ=255)
tCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTgg > 2:1567998/1‑76 (MQ=255)
tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGtttt < 2:281308/76‑1 (MQ=255)
aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTc < 2:904732/76‑1 (MQ=255)
gACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCa < 1:937833/76‑1 (MQ=255)
ccGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcgc > 2:885637/1‑76 (MQ=255)
cGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACcgcg > 2:819288/1‑76 (MQ=255)
cTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtt > 2:146847/1‑76 (MQ=255)
cTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtt > 1:1985969/1‑76 (MQ=255)
tGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt > 2:194518/1‑76 (MQ=255)
aaCACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTc < 1:1640524/76‑1 (MQ=255)
aaCACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTc > 2:265278/1‑76 (MQ=255)
aaCACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTc < 2:827364/76‑1 (MQ=255)
gcCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGaa < 1:48093/76‑1 (MQ=255)
cACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAcc > 1:1303223/1‑76 (MQ=255)
cGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg > 2:706872/1‑76 (MQ=255)
cGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg < 2:1442598/76‑1 (MQ=255)
aTACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt < 2:1195101/76‑1 (MQ=255)
aTACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt < 2:1262384/76‑1 (MQ=255)
ccTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcgc < 1:885637/76‑1 (MQ=255)
tACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGa > 2:1218250/1‑76 (MQ=255)
cGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGc > 1:940922/1‑76 (MQ=255)
cAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCg < 2:779839/76‑1 (MQ=255)
|
GACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCG > REL606/3251939‑3252084
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
