Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A38 F1 I1 R1
|
17 |
54.1 |
3391312 |
97.1% |
3292963 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,252,012 |
G→A |
A250V (GCT→GTT) |
nusA ← |
transcription elongation factor NusA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,252,012 | 0 | G | A | 100.0%
| 138.4
/ NA
| 36 | A250V (GCT→GTT) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/19); total (17/19) |
GACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCG > REL606/3251939‑3252084
|
gACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACa < 1:1454758/76‑1 (MQ=255)
gATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCt < 2:677055/76‑1 (MQ=255)
tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTAc < 2:787902/76‑1 (MQ=255)
atCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTAcc > 1:289750/1‑76 (MQ=255)
cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCgg > 1:1452543/1‑76 (MQ=255)
gATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGa < 2:159716/76‑1 (MQ=255)
aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGAt > 1:1599953/1‑76 (MQ=255)
tACCCTCGCCACCCAGTTCAGCAGACACCGCCTGAACGCGACCGCCACGCATACGCACGAAAACACCTACCGGacc > 2:283180/1‑74 (MQ=255)
cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATa < 1:146238/76‑1 (MQ=255)
cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATa < 2:163615/76‑1 (MQ=255)
cGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACg < 1:1328719/76‑1 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGttt > 2:1343401/1‑76 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGttt > 1:1572941/1‑76 (MQ=255)
aCCCAGTTCAGTAGACACCGCNGGAACACGCGCGCCACTCATACCTACGCAAACACCTACCGGATCGATACGTTTa < 2:279591/76‑1 (MQ=255)
cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTAt < 2:517187/76‑1 (MQ=255)
cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTAt > 1:544682/1‑76 (MQ=255)
ccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATc < 2:1113947/76‑1 (MQ=255)
aGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGt > 2:1009550/1‑76 (MQ=255)
gTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGtt > 1:984681/1‑76 (MQ=255)
gTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGtt > 1:45091/1‑76 (MQ=255)
cAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGt > 2:241063/1‑76 (MQ=255)
tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGtttt < 2:54295/76‑1 (MQ=255)
aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTc < 2:1594241/76‑1 (MQ=255)
gACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCa > 1:630942/1‑76 (MQ=255)
acacCGCCTGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCAc > 2:1385739/1‑76 (MQ=255)
tGAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAttt > 2:457891/1‑76 (MQ=255)
gAACACGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtttt > 1:1345205/1‑76 (MQ=255)
acGCGCGCCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCa < 1:111967/76‑1 (MQ=255)
gcgcgcCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACg < 2:801692/76‑1 (MQ=255)
cgcCACGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACgaga > 1:753395/1‑76 (MQ=255)
cGCATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg < 2:1175053/76‑1 (MQ=255)
cATACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGa < 2:358320/76‑1 (MQ=255)
aTACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt < 1:888804/76‑1 (MQ=255)
aTACCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt < 2:299994/76‑1 (MQ=255)
aCCTACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATcg > 2:160540/1‑76 (MQ=255)
tACGCAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGa > 2:11718/1‑76 (MQ=255)
cAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCg < 2:45091/76‑1 (MQ=255)
cAAACACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCg < 2:984681/76‑1 (MQ=255)
|
GACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCG > REL606/3251939‑3252084
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
