Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A24 F1 I1 R1
|
18 |
37.8 |
2419812 |
96.5% |
2335118 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,014,276 |
G→A |
G286D (GGT→GAT) |
ECB_02815 → |
Capsule polysaccharide export inner‑membrane protein kpsE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,014,276 | 0 | G | A | 100.0%
| 118.0
/ NA
| 33 | G286D (GGT→GAT) | ECB_02815 | Capsule polysaccharide export inner‑membrane protein kpsE |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/18); total (15/18) |
TGCGCGTAATGCGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGGTGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCGAGCTGT > REL606/3014205‑3014347
|
tGCGCGTAATGCGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGAc < 1:453860/76‑1 (MQ=255)
gcgcGTAATGCGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACa < 2:583740/76‑1 (MQ=255)
gcgcGTAATGCGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACa < 1:554065/76‑1 (MQ=255)
gTAATGCGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCt > 1:851538/1‑76 (MQ=255)
aTGCGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTaaa < 2:1203730/76‑1 (MQ=255)
tGCGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGCCAAGCTAAAc < 1:1117261/76‑1 (MQ=255)
cGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCg < 1:895314/76‑1 (MQ=255)
ttCAGTCATTGCAGGCACAAATTGACGNAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTAt > 1:1204183/1‑76 (MQ=255)
tCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATg > 1:383037/1‑76 (MQ=255)
aGTCAATGCAGGCACAAATTGACGCNGAAAAAAGCAAAATNACAGCGCTGCNGNATGACAAGCGAAACCGTATGGc > 2:20584/1‑76 (MQ=255)
gTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCg < 1:423023/76‑1 (MQ=255)
tGCAGGCACAAATTGACGAAGAAAAAAGAAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGa > 2:599309/1‑76 (MQ=255)
gCAGGCTCAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGAt < 1:926369/76‑1 (MQ=255)
cacaAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTga < 2:346113/76‑1 (MQ=255)
cacaAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGCCGGTGGATTTTga < 1:86397/76‑1 (MQ=255)
acaAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTgaa < 2:49981/76‑1 (MQ=255)
ttGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGaaat > 2:149948/1‑76 (MQ=255)
ttGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGaaat > 1:184779/1‑76 (MQ=255)
cGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGacatcaaa > 2:909439/1‑76 (MQ=255)
cGAAGAAAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGaaatcaaa > 2:909434/1‑76 (MQ=255)
aagaaAAAAGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGaaatcaaatc < 2:52608/76‑1 (MQ=255)
aaaaaaGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAAtcaaatcaaa < 1:477088/76‑1 (MQ=255)
aGCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTag < 1:236849/76‑1 (MQ=255)
gCAAAATCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTaga < 1:389438/76‑1 (MQ=255)
aaaaTCACAGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGt > 2:712579/1‑76 (MQ=255)
acaGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAaca > 2:479627/1‑76 (MQ=255)
caGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAacac > 2:415948/1‑76 (MQ=255)
aGCGCCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACAcc > 1:1161116/1‑76 (MQ=255)
gcgcCGCAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCg < 1:446450/76‑1 (MQ=255)
cgccgcAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCGa < 2:505533/76‑1 (MQ=255)
cgccgcAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCGa < 2:294982/76‑1 (MQ=255)
ccgcAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCGAGc < 1:82379/76‑1 (MQ=255)
cgcAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCGAGCt > 2:734485/1‑76 (MQ=255)
gcAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCGAGCTg > 2:471011/1‑76 (MQ=255)
cAGGATGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCGAGCTGt > 2:926884/1‑76 (MQ=255)
|
TGCGCGTAATGCGATTCAGTCATTGCAGGCACAAATTGACGAAGAAAAAAGCAAAATCACAGCGCCGCAGGGTGACAAGCTAAACCGTATGGCGGTGGATTTTGAAGAAATCAAATCAAAAGTAGAGTTCAACACCGAGCTGT > REL606/3014205‑3014347
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
