Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A24 F1 I1 R1
|
18 |
37.8 |
2419812 |
96.5% |
2335118 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,582 |
C→A |
A133S (GCT→TCT) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,582 | 0 | C | A | 100.0%
| 82.6
/ NA
| 23 | A133S (GCT→TCT) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (8/15); total (8/15) |
AGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCG > REL606/3328511‑3328654
|
aGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGccc < 2:365751/76‑1 (MQ=255)
aaaCCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTNAATCAGGAAGACTTCACGGGCACCAGAGCCCTg < 2:1024061/76‑1 (MQ=255)
cGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGcgcg > 2:605950/1‑76 (MQ=255)
ccAATGGGGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTg < 1:239954/76‑1 (MQ=255)
aaTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTgag < 2:139091/76‑3 (MQ=255)
ttGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGcgcg < 2:807169/76‑1 (MQ=255)
cGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCgcggcg < 1:692964/76‑1 (MQ=255)
gCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCaa < 2:549291/76‑1 (MQ=255)
ccATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAAc > 2:206403/1‑76 (MQ=255)
cATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCNGAGCCCTGCGAGGATACACGAATTGCGCGGCGTTNAAcc > 1:142755/1‑76 (MQ=255)
cGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTgg < 2:1076487/76‑1 (MQ=255)
ggTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTggg < 1:474667/76‑1 (MQ=255)
gTTCTTCAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGt > 1:488236/1‑76 (MQ=255)
tcttcAATCAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTcg > 1:290172/1‑76 (MQ=255)
cAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAcc < 2:818103/76‑1 (MQ=255)
cAGGAAGACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAcc < 2:942866/76‑1 (MQ=255)
gACTTCACGGGCACCATAGCCCTGCGCCGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAAACGGcaca > 1:60600/1‑76 (MQ=255)
gACTTCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGTGCGGCGTTCAACCTGGGTCGCGCCAACCGGcaca > 1:615194/1‑76 (MQ=255)
tCACGGGCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACaaa > 1:582438/1‑76 (MQ=255)
gggCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACTACCCAg > 1:1196559/1‑76 (MQ=255)
ggCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGa < 1:388216/76‑1 (MQ=255)
gCACCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCATCCTGGGTCGCGCCAACCGGCACATAAACCAGaa < 2:1035379/76‑1 (MQ=255)
aCCAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcg < 1:564736/76‑1 (MQ=255)
cAGAGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAcgcg < 1:582384/76‑1 (MQ=255)
|
AGAAACCGGCAGGCCAGCACCAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCG > REL606/3328511‑3328654
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
