Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A91 F1 I1 R1
|
18 |
55.4 |
3661525 |
95.1% |
3482110 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,219,546 |
T→G |
I97S (ATT→AGT) |
yjbA → |
phosphate‑starvation‑inducible protein PsiE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,219,546 | 0 | T | G | 94.8%
| 225.9
/ 1.1
| 58 | I97S (ATT→AGT) | yjbA | phosphate‑starvation‑inducible protein PsiE |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base G (35/20); total (37/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 |
CTGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCATTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTACT > REL606/4219473‑4219618
|
cTGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGtg < 1:297542/76‑1 (MQ=255)
tGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCATtgt > 2:1759502/1‑76 (MQ=255)
tGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCATtgt > 1:1719820/1‑76 (MQ=255)
gATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGtgtg > 2:1148875/1‑76 (MQ=255)
gATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGtgtg < 1:1630059/76‑1 (MQ=255)
ttGTGAATTTCTTTCAGTCCGTTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTgcg < 1:400820/76‑1 (MQ=255)
tgtgAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTgcgc > 2:164812/1‑76 (MQ=255)
tgAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCtt > 1:1052482/1‑76 (MQ=255)
tgAAGTACTTTCAGTCCGGTTTTCACGTCCCGCTCCGTTGCTTTGTCTATTTTGGGATCACCGCCAGTGTGCGCtt < 2:668019/76‑1 (MQ=255)
aaGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGa > 2:1535593/1‑76 (MQ=255)
aGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGat < 1:588428/76‑1 (MQ=255)
tACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGatca > 2:309689/1‑76 (MQ=255)
cTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGatcatc > 2:397663/1‑76 (MQ=255)
atttCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGatcatc > 1:959283/2‑76 (MQ=255)
ttCAGTCCTGTTTTCCCTTCCCGCTCGGTTACTTTGTCTATATTGGGATCGCCGCCATTGTGCGCTTGATCAtcgt < 1:131686/76‑1 (MQ=255)
ttCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATGGGGATCACCGCCAGTGTGCGCTTGATCAtcgt < 2:1315814/76‑1 (MQ=255)
cAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCAtcgtcg > 1:1405485/1‑76 (MQ=255)
cAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCAtcgtcg > 1:258294/1‑76 (MQ=255)
aGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGa > 1:492209/1‑76 (MQ=255)
tCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATc > 2:1229621/1‑76 (MQ=255)
ggTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATa < 2:1263172/76‑1 (MQ=255)
gTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATaa < 2:666408/76‑1 (MQ=255)
gTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATaa < 2:1577770/76‑1 (MQ=255)
ttttCACTTCCCTCTACGTTACTTTGTCTATATTGGGATCTCCGCCAGTGTGCGCTTGATCATCGTCGATCATaaa < 2:1628783/76‑1 (MQ=255)
ttttCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATaaa > 2:367041/1‑76 (MQ=255)
tttCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAAt < 2:1704398/76‑1 (MQ=255)
tttCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAAt < 1:1633155/76‑1 (MQ=255)
tCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGGCGATCATAAATCg > 1:919170/1‑76 (MQ=255)
cTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCa > 2:1170819/1‑76 (MQ=255)
ttCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCAc > 2:266907/1‑76 (MQ=255)
tCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACt > 2:1764932/1‑76 (MQ=255)
cccccCTACGTTACTTTGTCTATATTGGAATCCCCGCCAGTGTGCGCTGGATCTTCGTCGGTCATAAATCGCCACt < 2:914061/75‑1 (MQ=255)
ccGCTGCGTTTCTTTGTATATATGGGGATCCCCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTgg < 1:828611/76‑1 (MQ=255)
cGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGa > 1:58101/1‑76 (MQ=255)
cGCTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGa > 2:1334396/1‑76 (MQ=255)
ctgcGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtg > 1:1340384/4‑76 (MQ=255)
cTACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtg < 2:1088046/76‑1 (MQ=255)
tACGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgt < 1:1444825/76‑1 (MQ=255)
aCGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgtg > 1:768545/1‑76 (MQ=255)
aCGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgtg > 1:1328372/1‑76 (MQ=255)
aCGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgtg > 1:1294326/1‑76 (MQ=255)
aCGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGAtgtg > 1:969634/1‑76 (MQ=255)
cGTTACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATgtgt > 2:1171323/1‑76 (MQ=255)
ttACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTg > 2:1507137/1‑76 (MQ=255)
tACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGa > 1:1657011/1‑76 (MQ=255)
tACTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGa > 1:373638/1‑76 (MQ=255)
aCTTTGTTTATGTTGGAATCGCCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGAt < 1:480342/76‑1 (MQ=255)
aCTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGAt > 1:566332/1‑76 (MQ=255)
aCTTTGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGAt < 2:211186/76‑1 (MQ=255)
ttGTCTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTa < 1:1375466/76‑1 (MQ=255)
cTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCg > 2:1566703/1‑76 (MQ=255)
cTATATTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCg > 1:783860/1‑76 (MQ=255)
tataTTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCgg < 1:701519/76‑1 (MQ=255)
tataTTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCgg > 1:28953/1‑76 (MQ=255)
atatTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGc > 1:713242/1‑76 (MQ=255)
atatTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGc > 2:248926/1‑76 (MQ=255)
tatTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCt > 2:1304530/1‑76 (MQ=255)
tatTGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCt > 1:1412930/1‑76 (MQ=255)
tGGGATCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCg > 1:884076/1‑76 (MQ=255)
aTCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATcc > 2:553730/1‑76 (MQ=255)
tCACCGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATCCt > 2:1099761/1‑76 (MQ=255)
ccGCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCCACTCGGCTGCGATCCTGtt < 2:1469719/76‑1 (MQ=255)
gCCAGTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTAc < 1:1002577/76‑1 (MQ=255)
ccAGTGTGCGCTGGAGCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCGACTCGGCTGCGATCCTGTTACt < 2:319895/76‑1 (MQ=255)
|
CTGATTGTGAAGTACTTTCAGTCCGGTTTTCACTTCCCGCTACGTTACTTTGTCTATATTGGGATCACCGCCATTGTGCGCTTGATCATCGTCGATCATAAATCGCCACTGGATGTGTTGATCTACTCGGCTGCGATCCTGTTACT > REL606/4219473‑4219618
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
