Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A130 F1 I1 R1
|
13 |
250.2 |
15681636 |
95.5% |
14975962 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,161,948 |
G→A |
E365K (GAA→AAA) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,161,948 | 0 | G | A | 99.6%
| 922.0
/ ‑3.2
| 223 | coding (1093/4029 nt) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (158/64); minor base . (0/1); total (158/65) |
| Fisher's exact test for biased strand distribution p-value = 2.91e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.21e-01 |
Only 100 of 263 total aligned reads displayed.
TCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGT > REL606/4161875‑4162020
|
tCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTaaaa < 2:4688028/76‑1 (MQ=255)
tCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTaaaa < 2:2935881/76‑1 (MQ=255)
tCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTaaaa > 2:7262305/1‑76 (MQ=255)
ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATc > 2:69921/1‑76 (MQ=255)
ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATc > 2:2076392/1‑76 (MQ=255)
ggATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATc > 2:4492112/1‑76 (MQ=255)
gATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCt < 2:4909260/76‑1 (MQ=255)
aTCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTa > 2:2131764/1‑76 (MQ=255)
cACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTAcc > 1:1078692/1‑76 (MQ=255)
cACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTAcc > 2:2820061/1‑76 (MQ=255)
aCGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCg < 1:2521745/76‑1 (MQ=255)
ggCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCa > 1:4289042/1‑76 (MQ=255)
gCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCat > 2:1488432/1‑76 (MQ=255)
gCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCat > 2:4254389/1‑76 (MQ=255)
cccATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatg > 1:5240012/1‑76 (MQ=255)
ccATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatga > 1:4057065/1‑76 (MQ=255)
cATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatgat > 1:2527485/1‑76 (MQ=255)
atatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatgatg > 2:7095371/1‑76 (MQ=255)
atatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatgatg < 1:2866679/76‑1 (MQ=255)
atatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCatgatg > 2:1070554/1‑76 (MQ=255)
tatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATgc < 1:3710416/76‑1 (MQ=255)
tatatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATgc > 1:2435700/1‑76 (MQ=255)
tatCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATgcgc > 1:786533/1‑76 (MQ=255)
atCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGcc > 1:1548067/1‑76 (MQ=255)
tctcTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGccc > 2:1895393/1‑76 (MQ=255)
tctcTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGccc > 2:1404932/1‑76 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTg > 1:1741675/1‑76 (MQ=255)
tctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTg > 1:129607/1‑76 (MQ=255)
cctGAAACCCTACGGGGCGACCCAACCAACCGCCGTCTGAGCGCACCGGTAAAAATCTACCGCATGATGCGCCCTg < 1:701111/75‑1 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 2:3703242/1‑76 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 2:5192490/1‑76 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 1:3090839/1‑76 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 2:3313206/1‑76 (MQ=255)
ctGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTgg > 2:2977746/1‑76 (MQ=255)
tGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGc < 1:4126420/76‑1 (MQ=255)
tGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGc < 2:2383991/76‑1 (MQ=255)
aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGa > 2:6804424/1‑76 (MQ=255)
aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGa < 1:1469018/76‑1 (MQ=255)
aaaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGa < 1:1778584/76‑1 (MQ=255)
aaCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAg > 1:1518915/1‑76 (MQ=255)
aCCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgc > 2:6025647/1‑76 (MQ=255)
ccTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgcc > 2:2210049/1‑76 (MQ=255)
ccTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgcc > 1:4911538/1‑76 (MQ=255)
cTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgccg > 2:6591749/1‑76 (MQ=255)
tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgccgcc > 1:6428302/1‑76 (MQ=255)
tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgccgcc > 2:7639436/1‑76 (MQ=255)
tACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAgccgcc > 2:7551758/1‑76 (MQ=255)
ccGTGTCGCCCCAACTACCGCCCGTCGGAGCGCACTGGAAAAAATCTCCCGCATGATGCCCCCGGGCGAGccgccg < 2:6301460/75‑1 (MQ=255)
gtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAACAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTc > 2:3109896/1‑76 (MQ=255)
gtCGACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTc < 1:6317875/76‑1 (MQ=255)
tCGACCAAACTAACGACCGTCGGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCg < 2:573819/76‑1 (MQ=255)
gACCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTg < 1:7569032/76‑1 (MQ=255)
aCCCAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGa < 1:1209359/76‑1 (MQ=255)
cccAACAAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGaa < 1:357929/76‑1 (MQ=255)
cAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTCCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagc < 2:7041517/76‑1 (MQ=255)
cAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagc < 1:3872709/76‑1 (MQ=255)
cAACTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagc < 1:2022339/76‑1 (MQ=255)
aaCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagca < 2:666165/76‑1 (MQ=255)
aaCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagca > 2:5421169/1‑76 (MQ=255)
aCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcag > 1:1171105/1‑76 (MQ=255)
aCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcag > 1:186694/1‑76 (MQ=255)
aCTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcag > 1:5889529/1‑76 (MQ=255)
cTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcagc > 1:4861344/1‑76 (MQ=255)
cTAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAagcagc > 2:6161944/1‑76 (MQ=255)
cTAACGACCGTCTGAGAGCACTGGTAAAAATGTACCGCATGATGCGCCCTGGGGAGCCGCCGGCTCGTGAagcagc < 2:7804977/76‑1 (MQ=255)
tAACGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCg > 2:1347729/1‑76 (MQ=255)
aaCGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 2:363210/1‑76 (MQ=255)
aaCGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 2:4279566/1‑76 (MQ=255)
aaCGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 2:6266978/1‑76 (MQ=255)
aaCGACCGTCTGAGCACACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCgg > 1:6762871/1‑76 (MQ=255)
aCGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGa < 1:5723318/76‑1 (MQ=255)
cGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaa < 2:1763287/76‑1 (MQ=255)
cGACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaa > 1:322064/1‑76 (MQ=255)
gACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 1:55021/1‑76 (MQ=255)
gACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 2:494460/1‑76 (MQ=255)
gACCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa > 1:5825602/1‑76 (MQ=255)
gACCGTCTGAGCGCACTGGAAAAAATCTCCCGCATGAGGCCCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGaaa < 2:1669902/76‑1 (MQ=255)
aCCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAg < 1:1579796/76‑1 (MQ=255)
aCCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAg > 2:3933983/1‑76 (MQ=255)
aCCGTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAg < 1:7084340/76‑1 (MQ=255)
gccTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:1115460/74‑1 (MQ=255)
gTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 1:6650871/76‑1 (MQ=255)
gTCTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt > 2:5119283/1‑76 (MQ=255)
gTCTGAGCGCACTGGTAAAAATCTACCGCATGAGGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCt < 2:5924325/76‑1 (MQ=255)
tCTGAGCGCACTGGTAAAAATCTCCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTg < 1:7262305/76‑1 (MQ=255)
cTGAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGt > 1:979704/1‑76 (MQ=255)
tGAGCGCACTGGGAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt > 2:7505294/1‑76 (MQ=255)
tGAGCCCACTGGTAAAAATCTCCCGCATGATGCCCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGtt < 1:7346440/76‑1 (MQ=255)
gAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTc > 1:3785487/1‑76 (MQ=255)
gAGCGCACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTc > 2:437251/1‑76 (MQ=255)
gcgcACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCga < 2:7243792/76‑1 (MQ=255)
gcgcACTGGTAAAAATCTACCGCATGATGCCCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCga < 2:5542445/76‑1 (MQ=255)
cgcACTGGTAAAAATCTACCGCATGATGCCCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgag < 1:883196/76‑1 (MQ=255)
gcACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCgaga < 1:6914084/76‑1 (MQ=255)
cACTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGaa < 1:6111401/76‑1 (MQ=255)
cTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAcc > 2:2604735/1‑76 (MQ=255)
cTGGTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAAcc > 1:347469/1‑76 (MQ=255)
gggTAAAAATCTCCCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCt < 1:445446/75‑1 (MQ=255)
ggTAAAAATCTCCCGCATGATGCGCCCGGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTg < 2:6005155/76‑1 (MQ=255)
ggTAAAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTg < 2:2525786/76‑1 (MQ=255)
|
TCTGGATCACGGCCCATATATCTCTGAAACCTTACGTGTCGACCCAACTAACGACCGTCTGAGCGCACTGGTAGAAATCTACCGCATGATGCGCCCTGGCGAGCCGCCGACTCGTGAAGCAGCGGAAAGCCTGTTCGAGAACCTGT > REL606/4161875‑4162020
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
