Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A132 F1 I1 R1	21	265.3	16744490	95.4%	15974243	76.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,929,292	A→C	D359A (GAC→GCC)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,929,292	0	A	C	99.3%	1239.2 / ‑0.6	293	D359A (GAC→GCC)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (144/147); total (146/147)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

Only 100 of 311 total aligned reads displayed.

TTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCT  >  REL606/3929219‑3929364

 ttAAAGGTACAGGCAACATGGTACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTa                                                                       <  2:5055512/76‑1 (MQ=255)

 ttAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTa                                                                       <  2:2325241/76‑1 (MQ=255)

  tAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTAc                                                                      >  2:6686118/1‑76 (MQ=255)

   aaaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACa                                                                     <  2:4283305/76‑1 (MQ=255)

   aaaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACa                                                                     >  1:60740/1‑76 (MQ=255)

    aaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACaa                                                                    >  2:1422534/1‑76 (MQ=255)

    aaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACaa                                                                    <  1:738842/76‑1 (MQ=255)

    aaGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACaa                                                                    <  1:3562402/76‑1 (MQ=255)

       gTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCg                                                                 >  1:3372562/1‑76 (MQ=255)

        tACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGt                                                                >  2:8178671/1‑76 (MQ=255)

        tACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGt                                                                >  1:5295065/1‑76 (MQ=255)

         gcAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGtt                                                               >  1:3256825/2‑76 (MQ=255)

           aGGCAACATGGAACTGCCCCTCTCTCGTAAGATCGCTGAAAAACGCGGCTTCCCGGCTATCGCCTACAACCGTTCt                                                             <  2:7189621/76‑1 (MQ=255)

            ggCAACCTGGAACTGCCCCCCTCTTCTAAAAACGCTTTAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTg                                                            <  2:1978497/76‑1 (MQ=255)

            ggCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTg                                                            <  1:1577464/76‑1 (MQ=255)

              cAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGt                                                          >  1:2022199/1‑76 (MQ=255)

               aaCATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTa                                                         >  1:8185079/1‑76 (MQ=255)

               aaCATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTa                                                         >  1:903326/1‑76 (MQ=255)

               aaCATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTa                                                         >  1:6237817/1‑76 (MQ=255)

                  aTGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTAccc                                                      >  1:1221398/1‑76 (MQ=255)

                  aTGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTAccc                                                      >  2:1282714/1‑76 (MQ=255)

                  aTGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTAccc                                                      >  1:7976034/1‑76 (MQ=255)

                   tGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCCTTCCGGTACCCg                                                     >  1:2796621/1‑76 (MQ=255)

                    ggAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGt                                                    >  1:683693/1‑76 (MQ=255)

                      aaCTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTaa                                                  >  2:4567016/1‑76 (MQ=255)

                      aaCTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTaa                                                  <  1:1137518/76‑1 (MQ=255)

                       aCTGCCCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTaaa                                                 <  2:2960144/76‑1 (MQ=255)

                         tGCCCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaaga                                               <  2:4920734/76‑1 (MQ=255)

                         tGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaaga                                               >  2:3264952/1‑76 (MQ=255)

                         tGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaaga                                               >  1:2322096/1‑76 (MQ=255)

                          gccccTCTCTCTTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaagaa                                              <  2:3960682/73‑1 (MQ=255)

                           ccccTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaagaag                                             <  1:6952981/74‑1 (MQ=255)

                           cACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAaagaag                                             <  2:6862425/76‑1 (MQ=255)

                            aCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAagaaga                                            >  2:5972587/1‑76 (MQ=255)

                            aCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAagaaga                                            >  2:1670008/1‑76 (MQ=255)

                            aCCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAagaaga                                            >  2:8346320/1‑76 (MQ=255)

                             ccTCTCTCGTAAGATCGCTGAAAAACGCGTCTCCCCGGCTATCGCCTCCAACCGTTCTGGTACCCGTAAAGAagag                                           <  1:3811733/76‑1 (MQ=255)

                              ctctctCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAgc                                          <  2:3576911/76‑1 (MQ=255)

                                 tctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAgctgc                                       >  2:5230106/1‑76 (MQ=255)

                                  ctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct                                      >  1:2463712/1‑76 (MQ=255)

                                  ctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct                                      >  2:3883472/1‑76 (MQ=255)

                                  ctcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAgctgct                                      >  1:3183001/1‑76 (MQ=255)

                                   tcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTc                                     <  1:2903723/76‑1 (MQ=255)

                                   tcGTAAGACCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTc                                     <  2:448277/76‑1 (MQ=255)

                                   gcGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTc                                     <  2:6560804/75‑1 (MQ=255)

                                    cGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCa                                    >  1:7577256/1‑76 (MQ=255)

                                    cGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCCGCTCa                                    >  1:7690775/1‑76 (MQ=255)

                                    cGTAAGAACGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCa                                    <  1:3619029/76‑1 (MQ=255)

                                     gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                                   >  1:675382/1‑76 (MQ=255)

                                     gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                                   >  1:181404/1‑76 (MQ=255)

                                     gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                                   <  1:1921562/76‑1 (MQ=255)

                                     gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                                   <  1:3395045/76‑1 (MQ=255)

                                     gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                                   <  1:5748816/76‑1 (MQ=255)

                                     gTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCAc                                   >  1:5096555/1‑76 (MQ=255)

                                      tAAGATCGCTGAAAACCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACg                                  <  1:4400084/76‑1 (MQ=255)

                                      tAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACg                                  <  2:4674042/76‑1 (MQ=255)

                                      tAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACg                                  <  2:2494517/76‑1 (MQ=255)

                                       aaGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGa                                 <  2:800427/76‑1 (MQ=255)

                                       aaGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGa                                 >  2:3399873/1‑76 (MQ=255)

                                       aaGATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGa                                 >  1:3994649/1‑76 (MQ=255)

                                         gATCGCTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACt                               <  2:3150726/76‑1 (MQ=255)

                                           tCGCTGAAAACCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCa                             <  2:6375935/76‑1 (MQ=255)

                                           tCGCTGAAAAACCCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCa                             <  1:5937020/76‑1 (MQ=255)

                                             gcggAAAAACGCGTCTTCCCGTCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAgg                           <  2:278837/73‑1 (MQ=255)

                                             gCTGAAAAACGCGTCTCCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAgg                           <  1:4676770/76‑1 (MQ=255)

                                              cTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGga                          >  2:7452827/1‑76 (MQ=255)

                                              cTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGga                          >  1:4137421/1‑76 (MQ=255)

                                              cTGAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCCTAAAGAAGAGCTGCTCACGACTCAGga                          >  1:4565080/1‑76 (MQ=255)

                                                gAAAAACGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaag                        <  1:7832924/76‑1 (MQ=255)

                                                aaaaaaCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGgaag                        <  2:2852744/75‑1 (MQ=255)

                                                    aaCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACt                    >  2:544694/1‑76 (MQ=255)

                                                     aCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTg                   <  2:7043191/76‑1 (MQ=255)

                                                     aCGCGTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTg                   <  2:2704145/76‑1 (MQ=255)

                                                      cgcgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGc                  >  1:488456/1‑76 (MQ=255)

                                                      cgcgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGc                  <  2:6919249/76‑1 (MQ=255)

                                                       gcgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCa                 <  2:4866847/76‑1 (MQ=255)

                                                        cttCTTCCCGGTTATCTCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg                <  1:993543/74‑1 (MQ=255)

                                                        cgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg                <  2:5776664/76‑1 (MQ=255)

                                                        cgTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAg                <  2:8223250/76‑1 (MQ=255)

                                                         gTCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGa               <  2:7325362/76‑1 (MQ=255)

                                                          tCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaa              <  1:6981214/76‑1 (MQ=255)

                                                          tCTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaa              <  2:5563447/76‑1 (MQ=255)

                                                           cTTCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaaa             >  1:2097262/1‑76 (MQ=255)

                                                            ttCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaaaa            >  1:5662107/1‑76 (MQ=255)

                                                            ttCCCGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGaaaa            >  2:1060010/1‑76 (MQ=255)

                                                              cccGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtg          >  1:3130478/1‑76 (MQ=255)

                                                                cgccTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg        <  1:1282714/73‑1 (MQ=255)

                                                                cGGCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAAtgtg        >  2:1169019/1‑76 (MQ=255)

                                                                  gCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGa      >  2:6225534/1‑76 (MQ=255)

                                                                  gCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGa      <  2:5640922/76‑1 (MQ=255)

                                                                  gCTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGa      >  1:3696120/1‑76 (MQ=255)

                                                                   ttATCGCTTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt     <  2:7541736/75‑1 (MQ=255)

                                                                   cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt     >  1:720439/1‑76 (MQ=255)

                                                                   cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt     >  2:5902097/1‑76 (MQ=255)

                                                                   cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt     >  2:2183193/1‑76 (MQ=255)

                                                                   cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt     <  2:7642891/76‑1 (MQ=255)

                                                                   cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt     <  1:4231122/76‑1 (MQ=255)

                                                                   cTATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGAt     <  2:1581868/76‑1 (MQ=255)

                                                                    tATCGCCTACAACCGTTCTGGTGCCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATc    <  1:5168828/76‑1 (MQ=255)

                                                                    tATCGCCTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATc    >  1:6508565/1‑76 (MQ=255)

TTTAAAGGTACAGGCAACATGGAACTGCACCTCTCTCGTAAGATCGCTGAAAAACGCGTCTTCCCGGCTATCGACTACAACCGTTCTGGTACCCGTAAAGAAGAGCTGCTCACGACTCAGGAAGAACTGCAGAAAATGTGGATCCT  >  REL606/3929219‑3929364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A