breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 52,039 C→T L26F (CTT→TTT)  kefC → glutathione‑regulated potassium‑efflux system protein
RA 475,296 Δ1 bp coding (10/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
JC JC 627,132 IS150 (+) +3 bp intergenic (‑78/+34) rna ← / ← citT ribonuclease I/citrate:succinate antiporter
RA 795,728 (A)7→6 coding (602/993 nt) ECB_00737 ← hypothetical protein
MC JC 1,297,423 Δ10,224 bp [ychE][kch] [ychE], oppA, oppB, oppC, oppD, oppF, yciU, cls, [kch]
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,131,466 (A)9→8 intergenic (‑125/+175) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,815,253 G→T S6I (AGC→ATC)  ygdH → hypothetical protein
JC 3,145,967 +TAT coding (104/1842 nt) rpoD → RNA polymerase sigma factor
RA 3,495,641 T→C T799A (ACT→GCT)  glgP ← glycogen phosphorylase
RA 3,500,852 A→G I641T (ATT→ACT)  glgX ← glycogen debranching enzyme
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,929,292 A→C D359A (GAC→GCC)  rho → transcription termination factor Rho
RA 4,108,360 T→C N48S (AAC→AGC)  metJ ← transcriptional repressor protein MetJ
RA 4,164,603 T→C S1250P (TCT→CCT)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,201,918 C→A E214D (GAG→GAT iclR ← DNA‑binding transcriptional repressor
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2651655 =NA (NA)286 (1.120) 117/148 0.1 NA noncoding (910/1542 nt) rrsG 16S ribosomal RNA
?REL606 2774435 = NA (NA)noncoding (1443/1443 nt) IS150 repeat region
* ? REL606 3893554 =NA (NA)330 (1.300) 116/148 0.1 NA noncoding (1/1443 nt) IS150 repeat region
?REL606 3904522 = NA (NA)noncoding (908/1542 nt) rrsC 16S ribosomal RNA