breseq  version 0.33.1  revision 8505477f25b3 mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 232 shown, sorted by frequency from high to low)
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,515,909	0	A	C	57.7%	41.5 / 104.7	78	V260G (GTG→GGG)	ypfI	predicted hydrolase
*	REL606	720,466	0	G	C	55.0%	2.5 / 23.9	20	A23P (GCC→CCC)	phrB	deoxyribodipyrimidine photolyase, FAD‑binding
*	REL606	895,856	0	G	C	54.0%	17.8 / 66.1	37	A26P (GCC→CCC)	ECB_00836	putative Regulatory protein
*	REL606	720,464	0	C	G	50.0%	0.2 / 22.1	20	A22G (GCC→GGC)	phrB	deoxyribodipyrimidine photolyase, FAD‑binding
*	REL606	1,854,401	0	A	C	49.0%	86.1 / 60.8	96	K88N (AAA→AAC)	yeaO	hypothetical protein
*	REL606	4,180,249	0	T	G	47.8%	154.7 / 74.1	134	G24G (GGT→GGG)	yjaH	hypothetical protein
*	REL606	433	0	T	G	47.0%	97.8 / 46.0	83	V33G (GTG→GGG)	thrA	bifunctional aspartokinase I/homeserine dehydrogenase I
*	REL606	4,111,429	0	T	G	46.9%	115.5 / 15.6	81	G496G (GGT→GGG)	metL	bifunctional aspartate kinase II/homoserine dehydrogenase II
*	REL606	2,437,406	0	G	T	41.3%	31.1 / 36.2	46	D435E (GAC→GAA)	ypdD	fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*	REL606	931,314	0	T	C	39.1%	129.2 / 41.8	110	E84G (GAG→GGG)	ybjE	predicted transporter
*	REL606	1,982,697	0	T	G	38.0%	451.4 / 10.0	200	intergenic (+74/‑165)	asnT/yeeJ	tRNA‑Asn/adhesin
*	REL606	3,783,565	0	A	C	38.0%	149.2 / 51.6	100	T744P (ACA→CCA)	ECB_03521	conserved hypothetical protein
*	REL606	4,432,425	0	C	T	37.1%	233.3 / 122.0	151	E160K (GAA→AAA)	ppa	inorganic pyrophosphatase
*	REL606	955,035	0	A	C	35.7%	301.4 / 14.0	141	T192P (ACC→CCC)	lolA	outer‑membrane lipoprotein carrier protein precursor
*	REL606	2,376,348	0	T	G	35.7%	319.9 / 30.1	169	T63P (ACC→CCC)	folC	bifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*	REL606	1,505,284	0	A	G	35.1%	201.7 / 69.1	111	E46G (GAA→GGA)	ECB_01416	hypothetical protein
*	REL606	2,722,532	0	C	G	34.9%	111.7 / 33.4	63	G104G (GGC→GGG)	srlB	glucitol/sorbitol‑specific enzyme IIA component of PTS
*	REL606	390,957	0	A	C	34.6%	497.8 / 74.3	229	H7P (CAC→CCC)	malZ	maltodextrin glucosidase
*	REL606	1,632,964	0	T	G	34.2%	424.2 / 60.1	190	intergenic (‑96/‑39)	ynfA/ynfB	hypothetical protein/hypothetical protein
*	REL606	239,163	0	T	G	34.1%	410.5 / 128.9	211	V58G (GTG→GGG)	dnaQ	DNA polymerase III subunit epsilon

Marginal new junction evidence (sorted from low to high skew)
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	573170 =	141 (0.550)	7 (0.030) +CCTTCCGTATCTT	6/124	16.5	5.7%	coding (319/1599 nt)	ECB_00530	conserved hypothetical protein
	?	REL606	= 573174	141 (0.550)					coding (315/1599 nt)	ECB_00530	conserved hypothetical protein
*	?	REL606	4197881 =	366 (1.420)	26 (0.110)	4/134	18.6	6.8%	intergenic (+23/‑258)	aceA/aceK	isocitrate lyase/bifunctional isocitrate dehydrogenase kinase/phosphatase protein
	?	REL606	= 4603894	388 (1.690)					intergenic (+400/+20)	yjjV/yjjW	predicted DNase/predicted pyruvate formate lyase activating enzyme
*	?	REL606	2518512 =	305 (1.180)	141 (0.570)	3/144	20.0	28.8%	intergenic (‑66/+147)	purC/nlpB	phosphoribosylaminoimidazole‑ succinocarboxamidesynthase/lipoprotein
	?	REL606	= 2583279	406 (1.640)					intergenic (‑449/+3)	iscR/yfhQ	DNA‑binding transcriptional repressor/predicted methyltransferase