Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A105 F1 I1 R1
|
16 |
277.3 |
18051348 |
92.9% |
16769702 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,512,384 |
G→T |
intergenic (‑126/‑207) |
yhhX ← / → yhhY |
predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/predicted acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,512,384 | 0 | G | T | 99.5%
| 871.8
/ ‑3.8
| 220 | intergenic (‑126/‑207) | yhhX/yhhY | predicted oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/predicted acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (103/115); total (103/117) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01 |
Only 100 of 222 total aligned reads displayed.
GTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGG > REL606/3512311‑3512456
|
gTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATcc < 1:5410936/76‑1 (MQ=255)
gTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATcc < 2:8591101/76‑1 (MQ=255)
gTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATcc < 2:2124619/76‑1 (MQ=255)
ttCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCa > 2:3054119/1‑76 (MQ=255)
ttCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCa > 1:6978273/1‑76 (MQ=255)
tCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAg < 1:2351430/76‑1 (MQ=255)
ccTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGc > 2:1351638/1‑76 (MQ=255)
ccTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGc > 2:2501070/1‑76 (MQ=255)
ccTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGc < 2:1916421/76‑1 (MQ=255)
ccTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGc > 1:2642094/1‑76 (MQ=255)
ccTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGc > 2:4380915/1‑76 (MQ=255)
cTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCa > 2:3145293/1‑76 (MQ=255)
cTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑‑AAAAAAAATCCAGCAc < 1:7628219/76‑1 (MQ=255)
aCTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCAcc > 2:1105132/1‑76 (MQ=255)
cTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCAccc > 2:3802629/1‑76 (MQ=255)
tGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCg < 2:8125618/76‑1 (MQ=255)
tGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCg < 1:7202987/76‑1 (MQ=255)
tGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCg > 2:5336305/1‑76 (MQ=255)
aGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCggct > 2:3698318/1‑76 (MQ=255)
gTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAGAAAAAATCCAGCACCCggctg > 2:8039674/1‑76 (MQ=255)
gTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCggctg < 2:2007634/76‑1 (MQ=255)
gTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCggctg > 2:6481085/1‑76 (MQ=255)
gcATAACGAACGCAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTa < 2:367999/76‑1 (MQ=255)
aTAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAg < 1:2184021/76‑1 (MQ=255)
aTAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAg > 1:3013842/1‑76 (MQ=255)
aTAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAg < 1:3049617/76‑1 (MQ=255)
tAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGt > 1:1571411/1‑76 (MQ=255)
tAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGt < 2:1846000/76‑1 (MQ=255)
aaCGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTa > 1:4148040/1‑76 (MQ=255)
aCGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTaa < 1:4415620/76‑1 (MQ=255)
gAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATa > 2:6910462/1‑76 (MQ=255)
aaCACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATAc < 2:8203698/76‑1 (MQ=255)
aaCACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATAc > 2:4568892/1‑76 (MQ=255)
acacAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACt < 2:8541966/76‑1 (MQ=255)
acacAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACt < 1:3511774/76‑1 (MQ=255)
acacAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACt < 2:5380392/76‑1 (MQ=255)
acacAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACt < 1:453124/76‑1 (MQ=255)
cacaAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTg < 2:7088790/76‑1 (MQ=255)
cacaAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTg < 2:5497938/76‑1 (MQ=255)
cacaAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTg < 1:1220704/76‑1 (MQ=255)
acaAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTgg < 2:126561/76‑1 (MQ=255)
caAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGa < 1:3774934/76‑1 (MQ=255)
caAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGa < 1:1644044/76‑1 (MQ=255)
gCACTCCCGTGGATAAATTGAGAACGTAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGc < 1:6757676/76‑1 (MQ=255)
gCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGc < 1:6008934/76‑1 (MQ=255)
cACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCa < 2:4551597/76‑1 (MQ=255)
cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCggctg < 1:5367562/54‑1 (MQ=255)
cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAAt < 1:2115616/76‑1 (MQ=255)
cTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAAt > 1:4654606/1‑76 (MQ=255)
tCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAAtg > 2:6158792/1‑76 (MQ=255)
cccGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCaaagg > 2:8108913/1‑73 (MQ=255)
cccGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAAtgt > 2:8880557/1‑76 (MQ=255)
cGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGa > 2:3856966/1‑76 (MQ=255)
cGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGa > 1:2950102/1‑76 (MQ=255)
gTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAg > 2:6897052/1‑76 (MQ=255)
gTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAg > 1:2061584/1‑76 (MQ=255)
gTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAg > 2:3390079/1‑76 (MQ=255)
gTGGATAAATTGAGAACGAAAGATC‑‑AAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGc < 1:5029609/76‑1 (MQ=255)
tGGATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGc < 2:6525777/76‑1 (MQ=255)
gATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGcca > 2:2984884/1‑74 (MQ=255)
gATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCaa > 2:6650323/1‑76 (MQ=255)
gATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCaa > 1:774201/1‑76 (MQ=255)
gATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCaa < 1:3241658/76‑1 (MQ=255)
gATAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCaa < 2:407447/76‑1 (MQ=255)
aTAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAAt < 1:7845723/76‑1 (MQ=255)
tAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGGAATGTGAGCAATg > 2:4930516/1‑76 (MQ=255)
tAAATTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATg > 1:4161382/1‑76 (MQ=255)
aaaTTGAGAACGAAAGATCAAAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATg > 1:6253288/1‑76 (MQ=255)
aaaTTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGt > 2:6124461/1‑76 (MQ=255)
aaTTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTc > 2:6009839/1‑76 (MQ=255)
aaTTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTc > 2:182362/1‑76 (MQ=255)
aaTTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTc < 2:6392687/76‑1 (MQ=255)
aaTTGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTc < 2:4953341/76‑1 (MQ=255)
ttGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGt < 1:8710966/76‑1 (MQ=255)
tGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTg > 1:4077138/1‑76 (MQ=255)
tGAGAACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTg < 2:7909372/76‑1 (MQ=255)
gagaACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGc > 2:6279625/1‑76 (MQ=255)
gagaACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGc < 1:6184961/76‑1 (MQ=255)
agaACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCt > 2:884167/1‑76 (MQ=255)
agaACGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCt > 2:7282786/1‑76 (MQ=255)
aCGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTc < 1:3096229/76‑1 (MQ=255)
aCGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTc < 1:3570660/76‑1 (MQ=255)
aCGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTc < 1:932159/76‑1 (MQ=255)
aCGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTc < 1:8465551/76‑1 (MQ=255)
aCGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTc > 1:4276118/1‑76 (MQ=255)
aCGAAAGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTc < 1:446851/76‑1 (MQ=255)
aaaGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAgg > 1:112538/1‑76 (MQ=255)
aaaGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAgg < 1:6699742/76‑1 (MQ=255)
aaGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGt < 2:7391760/76‑1 (MQ=255)
aaGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGt < 2:7685730/76‑1 (MQ=255)
aaGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGt > 2:2929974/1‑76 (MQ=255)
aaGATC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGt > 2:4736532/1‑76 (MQ=255)
aTC‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTtct < 2:5826436/76‑1 (MQ=255)
c‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTTCTTTCAGGTTCTcc > 1:3261072/1‑76 (MQ=255)
c‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTcc < 1:6088256/76‑1 (MQ=255)
c‑AAAAAAAAATCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTcc > 2:5201959/1‑76 (MQ=255)
aaaaaaaaaTCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCcg > 1:8382648/1‑76 (MQ=255)
aaaaaaaaTCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCcgc < 1:8053484/76‑1 (MQ=255)
aaaaaaaaTCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCcgc > 1:100101/1‑76 (MQ=255)
aaaaaaTCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGa > 1:5485281/1‑76 (MQ=255)
|
GTTCCTACTGGAGTGCGCATAACGAACACAAGCACTCCCGTGGATAAATTGAGAACGAAAGATC‑AAAAAAAAAGCCAGCACCCGGCTGGCTAAGTAATACTGGAAGCAATGTGAGCAATGTCGTGCTTTCAGGTTCTCCGCGAGGG > REL606/3512311‑3512456
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
