Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A95 F1 I1 R1
|
16 |
38.4 |
2546724 |
96.0% |
2444855 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,873,040 |
C→A |
R332L (CGC→CTC) |
glmS ← |
D‑fructose‑6‑phosphate amidotransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,873,040 | 0 | C | A | 92.9%
| 140.1
/ 3.6
| 42 | R332L (CGC→CTC) | glmS | D‑fructose‑6‑phosphate amidotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base A (18/21); total (18/24) |
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 |
GGTATCCGCGGTTTCGCCAGACTGTGACAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCAT > REL606/3872967‑3873110
|
ggTATCCGCGGTTTCGCCAGACTGTGACAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAgg > 2:501030/1‑76 (MQ=255)
ggTATCCGCGGTTTCGCCAGACTGTGACAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAgg < 2:783566/76‑1 (MQ=255)
cgcgGTTTCGCCAGACTGTGACAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCa < 1:1106994/76‑1 (MQ=255)
gcgGTTTCGCCAGACTGTGACAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCag < 1:583672/76‑1 (MQ=255)
ttCGCCAGACTGTGACAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCg < 1:226841/76‑1 (MQ=255)
tgtgACAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGt > 1:1073547/1‑76 (MQ=255)
cAAGGTGATTATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCAc > 2:253431/1‑76 (MQ=255)
cAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCAc < 1:652926/76‑1 (MQ=255)
cAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCAc > 1:265182/1‑76 (MQ=255)
atcaGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTg < 2:13661/76‑1 (MQ=255)
tcaGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGc < 2:595559/76‑1 (MQ=255)
caGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCt < 1:240464/76‑1 (MQ=255)
caGGCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCt > 1:1245650/1‑76 (MQ=255)
ggCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAg < 2:472560/76‑1 (MQ=255)
gCTGTTACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGc > 1:665337/1‑76 (MQ=255)
tGTTACGACGCACNGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGa > 1:853225/1‑76 (MQ=255)
tACGACGCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTc > 2:788376/1‑76 (MQ=255)
acgacgCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCa < 1:323297/76‑1 (MQ=255)
acgacgCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCa < 1:1149873/76‑1 (MQ=255)
cgacgCACGGCAGGTTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGCAGTACTGGCTAGCGATTCaa > 2:1060945/1‑76 (MQ=255)
gacgCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCaaa > 1:1155967/1‑76 (MQ=255)
cgCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAAcc > 2:57363/1‑76 (MQ=255)
cgCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAAcc > 1:1258503/1‑76 (MQ=255)
cgCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCAACGTCGCACGGAATACCTGCTAGCGATTCAAAcc < 1:99790/76‑1 (MQ=255)
gCACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCa < 2:842698/76‑1 (MQ=255)
cACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAg < 1:629517/76‑1 (MQ=255)
cACGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAg > 2:481481/1‑76 (MQ=255)
aCGGCAGCTTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGt > 1:810413/1‑76 (MQ=255)
cGGCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTa < 2:446387/76‑1 (MQ=255)
ggCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAg < 2:364836/76‑1 (MQ=255)
ggCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGNTTCAAACCAGTAg < 1:857314/76‑1 (MQ=255)
ggCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAg < 2:788882/76‑1 (MQ=255)
ggCAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAg > 1:1053908/1‑76 (MQ=255)
cAGATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCg < 2:209751/76‑1 (MQ=255)
gATTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCggg < 2:865714/76‑1 (MQ=255)
aTTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGa > 1:737579/1‑76 (MQ=255)
aTTTGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGa > 1:565847/1‑76 (MQ=255)
tttGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGan < 1:337507/76‑2 (MQ=255)
ttGCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGaaa < 1:107204/76‑1 (MQ=255)
tGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAAc < 2:565025/76‑1 (MQ=255)
gCGATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAAcc > 2:145696/1‑76 (MQ=255)
gATAGAGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCAt < 2:1258503/76‑1 (MQ=255)
|
GGTATCCGCGGTTTCGCCAGACTGTGACAAGGTGATCATCAGGCTGTTACGACGCACGGCAGATTTGCGATAGCGGAATTCAGAGGCGATTTCGACGTCGCACGGAATACCTGCTAGCGATTCAAACCAGTAGCGGGAAACCAT > REL606/3872967‑3873110
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
