Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
17 |
37.0 |
2377229 |
96.2% |
2286894 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,161,306 |
C→T |
R151C (CGT→TGT) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,161,306 | 0 | C | T | 100.0%
| 113.5
/ NA
| 31 | R151C (CGT→TGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/16); total (15/16) |
AATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGC > REL606/4161233‑4161376
|
aaTTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGt < 2:731088/76‑1 (MQ=255)
aTTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTa < 1:403538/76‑1 (MQ=255)
cggCTCATGACAGACAACGGTGCCTTTGTGATAAACGGTATTGAGCGTATCATCGTTTCCCAGCTGCACTGTAGTc < 1:131363/74‑1 (MQ=255)
gCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCg < 2:529250/76‑1 (MQ=255)
cTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCgg > 2:913119/1‑76 (MQ=255)
cTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCgg > 1:627884/1‑76 (MQ=255)
agacaACGGTACCTTTGTTATCAGCGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGtcttc < 1:1147467/76‑1 (MQ=255)
aCGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGa > 1:705275/1‑76 (MQ=255)
ggTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACt > 1:537853/1‑76 (MQ=255)
ggTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACt < 2:72508/76‑1 (MQ=255)
tACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTATCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTcc > 2:739926/1‑76 (MQ=255)
cTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTAGACTCCGAc > 2:21233/1‑76 (MQ=255)
tttGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACa < 1:350454/76‑1 (MQ=255)
ttGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCATCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACaa < 1:876036/76‑1 (MQ=255)
ttGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACaa < 2:65739/76‑1 (MQ=255)
tGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACaaa > 1:1137287/1‑76 (MQ=255)
cAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTaaa < 2:101628/76‑1 (MQ=255)
cTGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCCTCTTTGACTCCGACAAAGGTAAAACCCActc > 1:843430/1‑76 (MQ=255)
tGAGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCActct > 2:777074/1‑76 (MQ=255)
aGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTc > 1:1150251/1‑76 (MQ=255)
aGCGTGTTATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTc < 2:584798/76‑1 (MQ=255)
tgtTATCGTTTTCCATCTGCACTGTAGTCCTGGCGGCTTCTTNGNCTCCGACNAAGGTAAAACCCACTCTTCGGGt < 1:1097437/76‑1 (MQ=255)
ttATCGTTTACCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTaa < 1:226310/76‑1 (MQ=255)
tATCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGCAAAACCCACTCTTCGGGTaaa > 1:444369/1‑76 (MQ=255)
tCGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGt > 2:574112/1‑76 (MQ=255)
cGTTTCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTg < 1:733653/76‑1 (MQ=255)
ttCCCAGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTg > 2:527149/1‑76 (MQ=255)
aGCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATaa < 1:753323/76‑1 (MQ=255)
gCTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAAc < 2:705275/76‑1 (MQ=255)
cTGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAAcg < 1:769334/76‑1 (MQ=255)
cTGCACTGTAGTCCGGGCGTCTACTTTGATTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGAATAGcg > 1:1031445/1‑76 (MQ=255)
tGCACTGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAAcgc > 1:504051/1‑76 (MQ=255)
|
AATTCCGCTCATGACAGACAACGGTACCTTTGTTATCAACGGTACTGAGCGTGTTATCGTTTCCCAGCTGCACCGTAGTCCGGGCGTCTTCTTTGACTCCGACAAAGGTAAAACCCACTCTTCGGGTAAAGTGCTGTATAACGC > REL606/4161233‑4161376
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
