Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A77 F1 I1 R1
|
21 |
53.0 |
3608474 |
95.6% |
3449701 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,101,777 |
A→C |
intergenic (‑7/+86) |
hslV ← / ← ftsN |
ATP‑dependent protease peptidase subunit/essential cell division protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,101,777 | 0 | A | C | 96.2%
| 203.5
/ ‑1.6
| 53 | intergenic (‑7/+86) | hslV/ftsN | ATP‑dependent protease peptidase subunit/essential cell division protein |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (21/30); total (22/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 |
ACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGACCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAGATGGGG > REL606/4101704‑4101847
|
aCGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGccc > 1:1310091/1‑76 (MQ=255)
ggCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCtt < 2:1441077/76‑1 (MQ=255)
gCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTg < 2:817426/76‑1 (MQ=255)
gCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTg < 1:1416273/76‑1 (MQ=255)
gCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTg < 1:389488/76‑1 (MQ=255)
tgcccATCGCCAGCGATTGCCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGtt < 2:166827/73‑1 (MQ=255)
gACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTa > 2:993561/1‑76 (MQ=255)
gACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTa > 2:440300/1‑76 (MQ=255)
ccATCGCCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACg < 2:601333/76‑1 (MQ=255)
aTGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTAc < 1:1136503/76‑1 (MQ=255)
aTGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTAc > 1:541191/1‑76 (MQ=255)
gACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACaa < 1:1525199/76‑1 (MQ=255)
aCCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACaaa < 2:571400/76‑1 (MQ=255)
cacaTGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAAcc < 1:942170/76‑1 (MQ=255)
caTGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACCGAGTACAAAcccc > 2:437100/1‑76 (MQ=255)
tGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGt < 1:276209/76‑1 (MQ=255)
ggCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTa > 2:1459320/1‑76 (MQ=255)
gCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTAc < 1:963175/76‑1 (MQ=255)
gCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTAc < 1:868447/76‑1 (MQ=255)
ccGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACa > 1:357316/1‑76 (MQ=255)
aCGGCGGGCGCTTACTATAGTTGTCACGAGCTGCCCCCGTGGTTACGAATACAGAGTACAAACCACGTACAAAAGt < 1:101247/76‑1 (MQ=255)
cGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTa < 2:1278548/76‑1 (MQ=255)
cGGCGTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTa > 2:1643358/1‑76 (MQ=255)
gTACGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACggg < 2:338913/76‑1 (MQ=255)
tACGCTTACTATAGTTGTCACGAGCTGACCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACgggg > 1:1228049/1‑76 (MQ=255)
tACCCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACgggg < 2:807371/76‑1 (MQ=255)
cGCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCa > 1:1247968/1‑76 (MQ=255)
gCTTACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCAt < 2:1580858/76‑1 (MQ=255)
ttACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATaa > 1:1697171/1‑76 (MQ=255)
ttACTATAGTTGTCACGAGCTGACCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATaa < 1:1563469/76‑1 (MQ=255)
tACTGTAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTGCAAAAGTACGGGGCATAAt < 2:668883/76‑1 (MQ=255)
tACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAAt > 1:1360460/1‑76 (MQ=255)
tACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAAt < 1:453823/76‑1 (MQ=255)
tACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGAACGGGGCATAAt > 1:255639/1‑76 (MQ=255)
tACTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGCACAAAAATACGGGGCATAAt > 1:1595953/1‑76 (MQ=255)
tACCATAGTTGTCACGCGCTGCCCCCTTGGTTACGAATACAGAGTACAACCCCCGTACAAAAGTACGGGGCATAAt < 2:581408/76‑1 (MQ=255)
aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg > 1:552449/1‑76 (MQ=255)
aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg < 1:1075491/76‑1 (MQ=255)
aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg < 1:1222836/76‑1 (MQ=255)
aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg < 1:194602/76‑1 (MQ=255)
aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg < 2:347917/76‑1 (MQ=255)
aCTATAGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATg > 1:754962/1‑76 (MQ=255)
taGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAt > 1:441361/1‑76 (MQ=255)
aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAtt < 2:1013100/76‑1 (MQ=255)
aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAtt > 1:879838/1‑76 (MQ=255)
aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAtt > 1:947938/1‑76 (MQ=255)
aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAAtt > 2:1550987/1‑76 (MQ=255)
aGTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAAAACGGGGCATAAGGCAAtt > 1:687333/1‑76 (MQ=255)
gTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATta > 1:1381464/1‑76 (MQ=255)
gTTGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATta < 2:848111/76‑1 (MQ=255)
ttGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATtat > 2:233525/1‑76 (MQ=255)
ttGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATtat < 2:520884/76‑1 (MQ=255)
ttGTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATtat < 2:554729/76‑1 (MQ=255)
gTCACGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAg < 2:1009737/76‑1 (MQ=255)
aggAGCTGCCCCCCTCGTTACGAAAACAGAGAACAAGCGCAGTAGAAAAGGCAAGAGCATAATGCAATTATATATg < 2:498227/74‑1 (MQ=255)
cGAGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAGATgg > 2:1655113/1‑76 (MQ=255)
aGCTGCCCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAGATgggg > 1:290327/1‑76 (MQ=255)
|
ACGTGGCCTGACCATCACCAGCGATGACCACATGGCCGTTACGGCGTACGCTTACTATAGTTGTCACGAGCTGACCCCTTGGTTACGAATACAGAGTACAAACCCCGTACAAAAGTACGGGGCATAATGCAATTATAGATGGGG > REL606/4101704‑4101847
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
