Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,968,180 T→G I85I (ATA→ATC)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,968,1800TG98.3% 617.7 / ‑0.0 175I85I (ATA→ATC) ptsPPEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base G (77/95);  total (80/95)
Fisher's exact test for biased strand distribution p-value = 9.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 175 total aligned reads displayed.

TTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCC  >  NC_000913/2968084‑2968276
                                                                                                |                                                                                                
ttGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATc                                                                                               >  1:1027486/1‑100 (MQ=255)
ttGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATc                                                                                               <  2:313930/100‑1 (MQ=255)
ttGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATc                                                                                               <  1:1979220/100‑1 (MQ=255)
ttGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATc                                                                                               <  1:4557080/100‑1 (MQ=255)
   aataatTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGt                                                                                            >  1:5481222/1‑100 (MQ=255)
     taatTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTc                                                                                          <  1:4816111/100‑1 (MQ=255)
      aatTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTcc                                                                                         <  1:1813061/100‑1 (MQ=255)
        ttGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGc                                                                                       >  2:4844193/1‑100 (MQ=255)
         tGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGcc                                                                                      <  1:1411610/100‑1 (MQ=255)
         tGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGcc                                                                                      >  1:1498911/1‑100 (MQ=255)
         tGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGcc                                                                                      <  2:324205/100‑1 (MQ=255)
           gTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAg                                                                                    <  2:1995518/100‑1 (MQ=255)
            tACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGc                                                                                   <  2:538437/100‑1 (MQ=255)
            tACGCCTAAAAACGCGAGGAAACGTTCTTCCTTTACGGGGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGc                                                                                   <  1:552817/100‑1 (MQ=255)
             aCGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGcc                                                                                  <  2:960305/100‑1 (MQ=255)
              cGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCt                                                                                 <  2:1961879/100‑1 (MQ=255)
               gCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTg                                                                                <  2:332206/100‑1 (MQ=255)
               gCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTg                                                                                >  1:1737560/1‑100 (MQ=255)
                ccTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCACGGTTGATCGGTTCCGCCAGCCTGc                                                                               >  1:1544439/1‑100 (MQ=255)
                ccTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGc                                                                               >  1:746707/1‑100 (MQ=255)
                 cTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGcc                                                                              >  2:2231824/1‑100 (MQ=255)
                 cTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGcc                                                                              >  1:1910009/1‑100 (MQ=255)
                  tAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCa                                                                             >  1:2776074/1‑100 (MQ=255)
                   aaaaaCGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCaa                                                                            <  1:1023877/100‑1 (MQ=255)
                      aaCGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCa                                                                         <  2:3185244/100‑1 (MQ=255)
                      aaCGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCa                                                                         <  2:3184029/100‑1 (MQ=255)
                       aCGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAATGTTGATCGGTTCCGCCAGCCTGCCAACCAg                                                                        <  1:16667/100‑1 (MQ=255)
                          cgcgGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGcc                                                                     >  2:1808959/1‑100 (MQ=255)
                          cgcgGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGcc                                                                     <  1:1788882/100‑1 (MQ=255)
                          cgcgGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGcc                                                                     <  1:3537129/100‑1 (MQ=255)
                          cgcgGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGcc                                                                     <  1:3803705/100‑1 (MQ=255)
                           gcgGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCcg                                                                    <  2:3731767/100‑1 (MQ=255)
                             ggAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCcgac                                                                  <  2:4550572/100‑1 (MQ=255)
                              gAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCcgacg                                                                 >  2:2477840/1‑100 (MQ=255)
                                 aCGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATc                                                              <  1:997976/100‑1 (MQ=255)
                                 aCGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATc                                                              >  1:3319454/1‑100 (MQ=255)
                                  cGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATcc                                                             <  1:2542260/100‑1 (MQ=255)
                                  cGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATcc                                                             >  2:2715724/1‑100 (MQ=255)
                                  cGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATcc                                                             <  1:4619089/100‑1 (MQ=255)
                                  cGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATcc                                                             <  1:581810/100‑1 (MQ=255)
                                    ttcttcTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCt                                                           <  1:4048524/100‑1 (MQ=255)
                                       ttcttTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTtca                                                        <  2:2322935/100‑1 (MQ=255)
                                        tcttTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCTAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTtcat                                                       >  2:5403751/1‑100 (MQ=255)
                                         cttTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTtcatc                                                      <  1:4315050/100‑1 (MQ=255)
                                             tACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAAc                                                  <  2:1061189/100‑1 (MQ=255)
                                               cGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAAcgc                                                >  1:4781723/1‑100 (MQ=255)
                                                ggAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAAcgcg                                               <  1:5084517/100‑1 (MQ=255)
                                                  aGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAg                                             <  1:2965906/100‑1 (MQ=255)
                                                   ggggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGc                                            >  2:2065884/1‑100 (MQ=255)
                                                   ggggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGc                                            <  2:2996404/100‑1 (MQ=255)
                                                     ggATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGt                                          >  1:3940208/1‑100 (MQ=255)
                                                       aTGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTa                                        <  2:748247/100‑1 (MQ=255)
                                                       aTGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTa                                        >  1:963196/1‑100 (MQ=255)
                                                        tGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTAc                                       >  2:5198405/1‑100 (MQ=255)
                                                        tGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTAc                                       <  2:3509757/100‑1 (MQ=255)
                                                         gTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACa                                      >  1:1717630/1‑100 (MQ=255)
                                                         gTATTTGAAGCTGGGGTGCTTGTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACa                                      <  1:819943/100‑1 (MQ=255)
                                                          tATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAg                                     <  1:3385962/100‑1 (MQ=255)
                                                          tATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAg                                     <  1:5160435/100‑1 (MQ=255)
                                                          tATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAg                                     <  2:2443792/100‑1 (MQ=255)
                                                           aTTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGt                                    >  1:3854549/1‑100 (MQ=255)
                                                           aTTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGt                                    >  2:1869655/1‑100 (MQ=255)
                                                           aTTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGt                                    >  1:5043315/1‑100 (MQ=255)
                                                           aTTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGt                                    <  1:3123810/100‑1 (MQ=255)
                                                              tGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCg                                 <  2:1149870/100‑1 (MQ=255)
                                                              tGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCg                                 <  1:317633/100‑1 (MQ=255)
                                                                aaGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGAc                               >  1:5413100/1‑100 (MQ=255)
                                                                aaGCAGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGAc                               >  2:4652613/1‑100 (MQ=255)
                                                                 aGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCTGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGAcc                              >  2:283593/1‑100 (MQ=255)
                                                                 aGCTGGGGTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGAcc                              >  2:3498196/1‑100 (MQ=255)
                                                                     ggggTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGt                          >  1:157665/1‑100 (MQ=255)
                                                                      gggTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTg                         <  2:1085539/100‑1 (MQ=255)
                                                                      gggTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTg                         <  2:2133806/100‑1 (MQ=255)
                                                                      gggTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTg                         <  1:3262857/100‑1 (MQ=255)
                                                                       ggTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTgg                        <  2:305535/100‑1 (MQ=255)
                                                                       ggTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTgg                        >  1:4712324/1‑100 (MQ=255)
                                                                        gTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGt                       >  2:4289755/1‑100 (MQ=255)
                                                                        gTGCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGt                       <  1:186868/100‑1 (MQ=255)
                                                                          gCTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGttt                     <  1:2741874/100‑1 (MQ=255)
                                                                           cTTTTGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGtttt                    >  2:4329502/1‑100 (MQ=255)
                                                                             tttGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGtttttt                  <  1:3899414/100‑1 (MQ=255)
                                                                              ttGCGCATCTGCAAGGTTGATCGGTTCCGTCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTc                 <  2:1549917/100‑1 (MQ=255)
                                                                              ttGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTc                 <  2:1294266/100‑1 (MQ=255)
                                                                               tGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGCCCGCGCGGTTTTTTCa                >  2:4601238/1‑100 (MQ=255)
                                                                               tGCGCATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCATTCATCAAACGCGAGCGTTACAGTGCGACGGCGTGGTTTTTTCa                >  2:16556/1‑100 (MQ=255)
                                                                                gcgcATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAg               >  1:4959750/1‑100 (MQ=255)
                                                                                 cgcATCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGc              >  2:453473/1‑100 (MQ=255)
                                                                                    aTCTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGcacc           >  2:808481/1‑100 (MQ=255)
                                                                                      cTGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCg         >  2:5343433/1‑100 (MQ=255)
                                                                                       tGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCgg        <  2:275660/100‑1 (MQ=255)
                                                                                       tGCAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCgg        >  2:2660782/1‑100 (MQ=255)
                                                                                        acAAGGTCGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCggg       >  2:755395/2‑100 (MQ=255)
                                                                                         cAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGt      >  1:5312979/1‑100 (MQ=255)
                                                                                         cAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGt      <  1:2390577/100‑1 (MQ=255)
                                                                                         cAAGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGt      <  1:4996739/100‑1 (MQ=255)
                                                                                           aGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCg    >  2:5290433/1‑100 (MQ=255)
                                                                                           aGGTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCg    >  2:62399/1‑100 (MQ=255)
                                                                                            ggTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGc   >  2:2917404/1‑100 (MQ=255)
                                                                                             gTTGATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGcc  >  1:2888374/1‑100 (MQ=255)
                                                                                             gTTGATCGGTTCCGCCAGCCTGACAACCAGGCCGACGATCCCTTCATCAAATGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGcc  >  1:894111/1‑100 (MQ=255)
                                                                                                |                                                                                                
TTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCC  >  NC_000913/2968084‑2968276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A