Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,237,742	A→C	K144N (AAA→AAC)	ygjR →	putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,237,742	0	A	C	99.2%	939.4 / ‑2.6	262	K144N (AAA→AAC)	ygjR	putative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (133/126); total (134/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 262 total aligned reads displayed.

AAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTC  >  NC_000913/3237646‑3237837

  aTCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCtt                                                                                            >  1:572509/1‑100 (MQ=255)

   tCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCttt                                                                                           <  1:5059546/100‑1 (MQ=255)

    cAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCtttt                                                                                          >  1:957461/1‑100 (MQ=255)

    cAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCtttt                                                                                          <  1:3622988/100‑1 (MQ=255)

    cAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCtttt                                                                                          >  2:3373578/1‑100 (MQ=255)

     aGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCttttt                                                                                         <  2:1521636/100‑1 (MQ=255)

      ggtagtACTGTTTGAGGCATTTAAAACCGCCTCCCTGCCGAACTCTCCTTTGTTGCGCCAGTCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTc                                                                                        >  2:2077635/1‑100 (MQ=255)

       gtggtgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCa                                                                                       >  1:2168010/1‑100 (MQ=255)

       gtggtgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCa                                                                                       <  2:1735294/100‑1 (MQ=255)

        tggtgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCaa                                                                                      >  1:772439/1‑100 (MQ=255)

        tggtgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCaa                                                                                      <  1:3990603/100‑1 (MQ=255)

          gtgCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACt                                                                                    <  1:3728374/100‑1 (MQ=255)

            gCTGTTTGAGGCATTTAAAATCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCATGCACTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTAt                                                                                  >  2:5481926/1‑100 (MQ=255)

            gCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTAt                                                                                  >  2:3948252/1‑100 (MQ=255)

             cTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTAtt                                                                                 >  1:47500/1‑100 (MQ=255)

             cTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTAtt                                                                                 >  1:640819/1‑100 (MQ=255)

               gTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGc                                                                               >  2:21174/1‑100 (MQ=255)

               gTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGc                                                                               >  1:2570279/1‑100 (MQ=255)

                 ttGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCa                                                                             >  2:5411671/1‑100 (MQ=255)

                  tgtggCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAg                                                                            <  1:3378632/97‑1 (MQ=255)

                   gAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGt                                                                           <  1:289882/100‑1 (MQ=255)

                   gAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGt                                                                           <  1:3286581/100‑1 (MQ=255)

                     tccATTTACATCCGCCTGCCGGCTGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGCCTTTTTCAACTCTTGCCAGTAt                                                                         <  2:4965899/98‑1 (MQ=255)

                     ggCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTAt                                                                         >  1:3407292/1‑100 (MQ=255)

                     ggCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTAt                                                                         >  2:5389667/1‑100 (MQ=255)

                     ggCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTAt                                                                         <  1:4393438/100‑1 (MQ=255)

                       cATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTc                                                                       >  2:2656782/1‑100 (MQ=255)

                       cATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTc                                                                       <  2:3765121/100‑1 (MQ=255)

                       cATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTc                                                                       >  1:5431816/1‑100 (MQ=255)

                       cATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTc                                                                       <  1:4247133/100‑1 (MQ=255)

                       cATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTc                                                                       >  1:1243384/1‑100 (MQ=255)

                        aTTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTTAACTATTGCCAGTATTcc                                                                      <  1:4707244/100‑1 (MQ=255)

                        aTTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTCTTcc                                                                      >  1:1118114/1‑100 (MQ=255)

                        aTTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTcc                                                                      >  2:86003/1‑100 (MQ=255)

                        aTTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTcc                                                                      >  1:2038612/1‑100 (MQ=255)

                        aTTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTcc                                                                      >  1:5196359/1‑100 (MQ=255)

                        aTTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTAGGCAAACTGCGGAACGTCTTTTTCAACTCTTGCCAGTATTcc                                                                      >  2:4452208/1‑100 (MQ=255)

                         tttAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCt                                                                     >  2:5126808/1‑100 (MQ=255)

                         tttAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCt                                                                     >  1:4286399/1‑100 (MQ=255)

                           tAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTcg                                                                   >  1:1865373/1‑100 (MQ=255)

                           tAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTcg                                                                   >  2:2585376/1‑100 (MQ=255)

                           tAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTcg                                                                   >  1:3971655/1‑100 (MQ=255)

                           tAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTcg                                                                   >  2:2943889/1‑100 (MQ=255)

                           tAAAACCGCCTGCCTGCCGAACTTTCATCTGTTGCACCAGGCGCTGCCGAGAGTCGGCAAACTGCGCTACGTCTTTTTCAACTATTGCCAGTATTCCTcg                                                                   >  1:1927018/1‑100 (MQ=255)

                            aaaaCCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTcgc                                                                  >  2:1309231/1‑100 (MQ=255)

                            aaaaCCGCCTGCCGGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTcgc                                                                  <  2:4189264/100‑1 (MQ=255)

                                    ctgcctgcCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAGAGTCGGCGAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCaa                                                          >  1:5289168/1‑100 (MQ=255)

                                    ctgcctgcCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCaa                                                          >  1:3183896/1‑100 (MQ=255)

                                     tgcctgccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAAc                                                         >  1:937696/1‑100 (MQ=255)

                                      gcctgccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACg                                                        >  2:3656545/1‑100 (MQ=255)

                                      gcctgccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACg                                                        <  2:156119/100‑1 (MQ=255)

                                       cctgccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGt                                                       <  2:3038316/100‑1 (MQ=255)

                                        ctgccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGtt                                                      >  2:2388161/1‑100 (MQ=255)

                                        ctgccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGtt                                                      >  2:4682392/1‑100 (MQ=255)

                                        ctgccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGtt                                                      >  2:1932547/1‑100 (MQ=255)

                                          gccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTAc                                                    >  2:3893137/1‑100 (MQ=255)

                                           ccGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTAcc                                                   <  1:5162710/100‑1 (MQ=255)

                                             gAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTg                                                 <  1:826451/100‑1 (MQ=255)

                                              aaCTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTgg                                                <  1:1728479/100‑1 (MQ=255)

                                                cTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGAt                                              <  1:5493712/100‑1 (MQ=255)

                                                 tttCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATg                                             >  1:752032/1‑100 (MQ=255)

                                                  ttCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATgg                                            <  1:4320277/100‑1 (MQ=255)

                                                  ttCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATgg                                            <  1:4803619/100‑1 (MQ=255)

                                                  ttCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATgg                                            <  2:2186572/100‑1 (MQ=255)

                                                  ttCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATgg                                            >  2:2293567/1‑100 (MQ=255)

                                                   tCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTCCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGt                                           <  2:3537704/100‑1 (MQ=255)

                                                    cATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTg                                          >  2:3101543/1‑100 (MQ=255)

                                                     aTTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTga                                         <  2:1424493/100‑1 (MQ=255)

                                                     aTTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTga                                         <  1:1340975/100‑1 (MQ=255)

                                                        tgttgCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGaa                                      >  1:1161635/1‑100 (MQ=255)

                                                         gttgCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAAt                                     >  1:2781416/1‑100 (MQ=255)

                                                           tgCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTTTTTTTTTTTCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATcc                                   >  2:5047347/1‑100 (MQ=255)

                                                           tgCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATcc                                   <  2:45939/100‑1 (MQ=255)

                                                             cgcCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCa                                 <  2:5257380/100‑1 (MQ=255)

                                                              gcCCGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATGGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTCAGAATCCCaa                                <  1:4545337/100‑1 (MQ=255)

                                                               ccAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAac                               <  2:605934/100‑1 (MQ=255)

                                                               ccAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAac                               <  2:421508/100‑1 (MQ=255)

                                                               ccAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAac                               <  2:925316/100‑1 (MQ=255)

                                                               ccAGGCGCTGCCGAAAGTCGGCAAACTGCGGAACGTC‑TTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAaca                              >  1:4655713/1‑100 (MQ=255)

                                                                  ggCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACAcc                            >  2:5500706/1‑100 (MQ=255)

                                                                  ggCGCTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACAcc                            <  2:5176070/100‑1 (MQ=255)

                                                                   gcgcTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCt                           <  2:4836083/100‑1 (MQ=255)

                                                                     gcTGCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCttt                         <  2:1226854/100‑1 (MQ=255)

                                                                      cTGCCGAAAGTCGGCAAACTGCGGAACGTCCTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTa                        >  1:3216281/1‑100 (MQ=255)

                                                                        gCCGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAAt                      >  1:2440815/1‑100 (MQ=255)

                                                                         ccGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATc                     >  2:2246770/1‑100 (MQ=255)

                                                                          cGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATcc                    >  2:3326316/1‑100 (MQ=255)

                                                                          cGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATcc                    >  2:2454822/1‑100 (MQ=255)

                                                                          cGAAAGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATcc                    >  1:4145642/1‑100 (MQ=255)

                                                                           gAAAGTCGGCAGACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCg                   <  2:2483658/100‑1 (MQ=255)

                                                                             aaGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGc                 <  2:1834708/100‑1 (MQ=255)

                                                                             aaGTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGc                 <  1:1665/100‑1 (MQ=255)

                                                                               gTCGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCAt               >  1:3640771/1‑100 (MQ=255)

                                                                                 cGGCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATtc             >  2:114212/1‑100 (MQ=255)

                                                                                  ggCAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATtct            <  1:2317134/100‑1 (MQ=255)

                                                                                    cAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTctct          <  1:2530759/100‑1 (MQ=255)

                                                                                    cAAACTGCGGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTctct          <  1:3056118/100‑1 (MQ=255)

                                                                                      accTGCAGAACGTCTGTTTCAACTATTGCCATTATTCCTCGCGGTATCAACGTTACCTGCATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTaa        <  2:4616064/98‑1 (MQ=255)

                                                                                       aCTGCAGAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGTTGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAAc       <  2:830904/100‑1 (MQ=255)

                                                                                            ggAACGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTc  >  1:1000952/1‑100 (MQ=255)

AAATCAGGTGGTGCTGTTTGAGGCATTTAAAACCGCCTGCCTGCCGAACTTTCATTTGTTGCGCCAGGCGCTGCCGAAAGTCGGCAAACTGCGGAAAGTCTTTTTCAACTATTGCCAGTATTCCTCGCGGTATCAACGTTACCTGGATGGTGAGAATCCCAACACCTTTAATCCGGCATTCTCTAACGGTTC  >  NC_000913/3237646‑3237837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A