Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I3 R1 53 201.3 10250884 92.1% 9441064 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,240,984 (G)7→8 coding (1041/1245 nt) sstT → sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,240,9771.G96.1% 531.6 / 14.3 153coding (1034/1245 nt)sstTsodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base . (2/4);  new base G (63/84);  total (65/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

TGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑G‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCG  >  NC_000913/3240878‑3241075
                                                                                                    |                                                                                                    
tggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGc                                                                                                       <  2:1679387/100‑1 (MQ=255)
 ggctgCTGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g                                                                                                     <  2:1607613/100‑1 (MQ=255)
  gctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g‑g                                                                                                   >  2:4107321/1‑100 (MQ=255)
       ggTTAATACGCTGTGTATTCCGGTCGATCTGCCCACGGCGCTGCGGATGAGCGTGGTGGCTTGCCTGTGTGCCTGTGGCGCATCCTGCTGGGC‑g‑gggcga                                                                                              >  2:4075179/1‑97 (MQ=255)
       ggTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g‑gggg‑gg                                                                                             >  2:1100800/1‑100 (MQ=255)
       ggTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g‑gggg‑gg                                                                                             <  2:28670/100‑1 (MQ=255)
       ggTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g‑gggg‑gg                                                                                             <  2:1800952/100‑1 (MQ=255)
        gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g‑gggg‑ggg                                                                                            <  1:532747/100‑2 (MQ=255)
            aTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGGGCGAGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑g‑gggg‑gggtctc                                                                                        >  1:4528130/1‑95 (MQ=255)
                gCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgct                                                                                     <  2:789315/100‑1 (MQ=255)
                 ntGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgctt                                                                                    >  1:1383279/2‑99 (MQ=255)
                 cTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgctg                                                                                    >  1:5013744/1‑100 (MQ=255)
                  tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgctgc                                                                                   <  1:690504/100‑1 (MQ=255)
                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgctgct                                                                                  <  2:3369084/100‑1 (MQ=255)
                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgctgct                                                                                  >  2:4197269/1‑100 (MQ=255)
                   gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgctgct                                                                                  >  1:3427736/1‑100 (MQ=255)
                    ggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgctgctg                                                                                 <  1:4673548/100‑1 (MQ=255)
                    ggTATGCCGGTCGATCTGCCCACGGTGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTTGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTctgctgctg                                                                                 >  2:1405725/1‑100 (MQ=255)
                      tATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGAt                                                                               <  1:2028449/100‑1 (MQ=255)
                       aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATc                                                                              <  1:4730506/100‑1 (MQ=255)
                       aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTATGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCCGCTGATc                                                                              <  1:1119199/100‑1 (MQ=255)
                        ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATcc                                                                             >  1:814927/1‑100 (MQ=255)
                        ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATcc                                                                             >  2:4595410/1‑100 (MQ=255)
                        ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATcc                                                                             <  2:4589394/100‑1 (MQ=255)
                         tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATccc                                                                            <  2:2822978/100‑1 (MQ=255)
                         tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATccc                                                                            <  1:3361281/100‑1 (MQ=255)
                         tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATccc                                                                            <  1:1783263/100‑1 (MQ=255)
                         tCCGGGCGATCTGCCACCGGCGATACCGTTGAGCGTGGTGGCTTGCATGTGTGCCTGCAGCGCATCCGGCGTGGC‑GAGGGG‑GGTCTCTGCTGCTGATccc                                                                            <  2:1000114/100‑1 (MQ=255)
                           cGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGGTCCCAc                                                                          >  2:4154644/1‑100 (MQ=255)
                           cGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCAc                                                                          <  1:1681478/100‑1 (MQ=255)
                            ggTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACt                                                                         >  1:2591483/1‑100 (MQ=255)
                            ggTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACt                                                                         >  1:2207335/1‑100 (MQ=255)
                             gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTg                                                                        >  1:3892404/1‑100 (MQ=255)
                             gTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTg                                                                        <  1:2449037/100‑1 (MQ=255)
                                gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGcc                                                                     <  1:3695842/100‑1 (MQ=255)
                                gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGcc                                                                     <  2:131573/100‑1 (MQ=255)
                                gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGcc                                                                     >  2:3987632/1‑100 (MQ=255)
                                gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGcc                                                                     <  1:640126/100‑1 (MQ=255)
                                 aTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGccc                                                                    >  2:1903876/1‑99 (MQ=255)
                                 aTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCt                                                                    >  1:2947716/1‑100 (MQ=255)
                                  tCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTg                                                                   <  1:980286/100‑1 (MQ=255)
                                    tGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                 >  1:5046295/1‑100 (MQ=255)
                                    tGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGGGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                                 <  1:2627695/100‑1 (MQ=255)
                                     gCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTaa                                                                >  2:87592/1‑100 (MQ=255)
                                       ccACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAata                                                              <  1:996632/100‑1 (MQ=255)
                                       ccACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAata                                                              <  2:2806838/100‑1 (MQ=255)
                                       ccACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAata                                                              <  1:257543/100‑1 (MQ=255)
                                        cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAatat                                                             <  2:3881427/100‑1 (MQ=255)
                                          cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGt                                                           >  1:2723800/1‑100 (MQ=255)
                                            gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                         <  2:3605234/100‑1 (MQ=255)
                                            gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                         <  2:3280831/100‑1 (MQ=255)
                                             cgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTATTATGTTCg                                                        >  1:1437033/1‑100 (MQ=255)
                                             cgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCg                                                        <  1:366017/100‑1 (MQ=255)
                                                tgctgTTGAGCGTGGTGGCTGCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑G‑GGGGCGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTa                                                     >  2:5065891/1‑100 (MQ=255)
                                                 gctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTAt                                                    <  1:2724840/100‑1 (MQ=255)
                                                    gTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTc                                                 <  2:1972029/100‑1 (MQ=255)
                                                     ttGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCg                                                <  1:608112/100‑1 (MQ=255)
                                                      tGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGa                                               >  1:2111452/1‑100 (MQ=255)
                                                      ngAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGa                                               >  1:1059209/2‑100 (MQ=255)
                                                        aGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAAc                                             <  1:1473911/100‑1 (MQ=255)
                                                        aGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAAc                                             <  2:2929173/100‑1 (MQ=255)
                                                        aGCGTGGTGGCTTCTCTGGGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTCCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAAc                                             <  1:4870560/100‑1 (MQ=255)
                                                         gCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACg                                            <  2:3389869/100‑1 (MQ=255)
                                                              gtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATc                                       >  2:3481710/1‑100 (MQ=255)
                                                              gtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATc                                       >  2:244414/1‑100 (MQ=255)
                                                              gtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATc                                       <  1:2716677/100‑1 (MQ=255)
                                                               tggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCg                                      <  2:1494167/100‑1 (MQ=255)
                                                                 gCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGcc                                    >  1:287804/1‑100 (MQ=255)
                                                                 gCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGcc                                    >  2:2549303/1‑100 (MQ=255)
                                                                  cTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                                   >  2:4801184/1‑100 (MQ=255)
                                                                  cTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCAGATCCCACTGGCATGTAATATGTTCGGTATTTCGAACGATATCGccc                                   >  2:3000631/1‑99 (MQ=255)
                                                                   ttCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCAt                                  >  2:1469817/1‑100 (MQ=255)
                                                                   ttCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCAt                                  >  2:741807/1‑100 (MQ=255)
                                                                    tctcTGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATg                                 <  1:4736882/100‑1 (MQ=255)
                                                                      tctGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGTACGATATCGCCATGCa                               <  2:4913750/100‑1 (MQ=255)
                                                                       ctGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                              <  2:2041907/100‑1 (MQ=255)
                                                                       ctGTGTGCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                              >  1:1047000/1‑100 (MQ=255)
                                                                        tgtgtgCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAgg                             <  1:2579038/100‑1 (MQ=255)
                                                                         gtgtgCCTGTGGCGCCTCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATCTGTTCGGTATTTCGAACGATATCGCCATGCAggt                            <  2:2422561/100‑1 (MQ=255)
                                                                           gtgCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtgg                          >  1:3198641/1‑100 (MQ=255)
                                                                            tgCCTGTGGGGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtggt                         <  2:2217878/100‑1 (MQ=255)
                                                                            tgCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtggt                         <  1:3157489/100‑1 (MQ=255)
                                                                             gCCTGTGGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGtt                        >  1:2828868/1‑100 (MQ=255)
                                                                                  tgGCGCATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                   <  1:654842/100‑1 (MQ=255)
                                                                                     cgcATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCgg                <  2:3138021/100‑1 (MQ=255)
                                                                                      gcATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGc               <  1:4156009/100‑1 (MQ=255)
                                                                                      gcATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGc               <  1:4155269/100‑1 (MQ=255)
                                                                                      gcATCCGGCGTGGCGG‑GGGG‑GGTCACTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGc               <  2:827223/100‑1 (MQ=255)
                                                                                       cATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCGCTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt              <  1:1995265/100‑1 (MQ=255)
                                                                                       cATCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt              <  1:1909374/100‑1 (MQ=255)
                                                                                         tCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCttt            >  2:4501836/1‑100 (MQ=255)
                                                                                         tCCGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCttt            >  2:2189000/1‑100 (MQ=255)
                                                                                          ccGGCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTa           <  1:1133799/100‑1 (MQ=255)
                                                                                           cGGCGTGGCGG‑GGGG‑GGTCTCTTCTGCTGATCCCACTGGCCTGTAATATGTGCGGTATTTCGAACTATATCGCCATGCGGGTTTTTGCCGTCTGCTTTat          >  2:3057199/1‑100 (MQ=255)
                                                                                            ggCGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatc         <  2:444283/100‑1 (MQ=255)
                                                                                              cGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat       <  2:284347/100‑1 (MQ=255)
                                                                                              cGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat       <  2:1377232/100‑1 (MQ=255)
                                                                                              cGTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat       >  1:3910082/1‑100 (MQ=255)
                                                                                               gTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcatc      <  1:4264903/100‑1 (MQ=255)
                                                                                               gTGGCGG‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcatc      <  2:2657614/100‑1 (MQ=255)
                                                                                                    |                                                                                                    
TGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑G‑GGGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCG  >  NC_000913/3240878‑3241075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A