Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)_7→8	coding (1041/1245 nt)	sstT →	sodium:serine/threonine symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	94.6%	563.3 / 24.8	166	coding (1034/1245 nt)	sstT	sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand): ref base . (4/5); new base G (84/73); total (88/78)
Fisher's exact test for biased strand distribution p-value = 7.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

GGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATC  >  NC_000913/3240879‑3241071

  ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑gg‑g                                                                                              >  1:4243577/1‑100 (MQ=255)

  ctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑gg‑g                                                                                              <  2:2239906/100‑1 (MQ=255)

    gCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑gg‑ggg                                                                                            <  1:3090702/100‑1 (MQ=255)

    gCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑gg‑ggg                                                                                            >  1:2812876/1‑100 (MQ=255)

       gTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑gg‑ggg‑ggg                                                                                        <  1:3777922/100‑2 (MQ=255)

         tAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑gg‑ggg‑gggtc                                                                                      <  2:1700193/100‑4 (MQ=255)

          aaTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑gg‑ggg‑gggtct                                                                                     >  1:444565/1‑96 (MQ=255)

           aTACGCTTGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑gg‑ggg‑gggtctc                                                                                    <  1:4627987/100‑6 (MQ=255)

            tACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑GG‑GGGAGGTctct                                                                                    <  2:1630598/100‑1 (MQ=255)

                 tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTctgctgc                                                                               <  2:3785984/100‑1 (MQ=255)

                 tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGGTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTctgctgc                                                                               >  2:5358058/1‑100 (MQ=255)

                  gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGCGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTctgctgct                                                                              <  2:5001966/100‑1 (MQ=255)

                  gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTctgctgct                                                                              <  1:333527/100‑1 (MQ=255)

                   ggTATTGCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTctgctgctg                                                                             <  2:2984836/100‑1 (MQ=255)

                      aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATc                                                                          <  1:175568/100‑1 (MQ=255)

                      aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATc                                                                          <  2:494455/100‑1 (MQ=255)

                      aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATc                                                                          >  2:266962/1‑100 (MQ=255)

                      aTTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATc                                                                          >  1:4764630/1‑100 (MQ=255)

                       ttCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATcc                                                                         >  1:5128718/1‑100 (MQ=255)

                        tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATccc                                                                        <  1:5408985/100‑1 (MQ=255)

                        tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATccc                                                                        <  2:296919/100‑1 (MQ=255)

                        tCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATccc                                                                        <  2:5221767/100‑1 (MQ=255)

                         ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCa                                                                       >  1:1601612/1‑100 (MQ=255)

                         ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCa                                                                       >  1:1375556/1‑100 (MQ=255)

                         ccGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCa                                                                       >  2:4119997/1‑100 (MQ=255)

                           tgTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACt                                                                     <  2:4819199/99‑1 (MQ=255)

                           ggTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACt                                                                     <  1:2504865/100‑1 (MQ=255)

                               taTCTCCCCACGGTGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTACCTGTGGCGCATCCGGC‑GTGGC‑‑GG‑GGGCGGTCTCTGCTGTTGAGCCCACTGGcc                                                                 <  2:5126451/99‑1 (MQ=255)

                               gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGcc                                                                 <  1:1587861/100‑1 (MQ=255)

                               gATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGcc                                                                 <  1:1799614/100‑1 (MQ=255)

                                aTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCt                                                                >  1:4291532/1‑100 (MQ=255)

                                aTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCATGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCt                                                                >  1:4187896/1‑100 (MQ=255)

                                aTCCGCCCACGGCGCTGGTGTTGGGGGTGGGGGCTTCTCTGTGTGCCTGTGGCGCATCCGGG‑GTGGCGGGG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGcc                                                                 <  1:2985457/100‑1 (MQ=255)

                                  cTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGt                                                              >  2:4141494/1‑100 (MQ=255)

                                   tGCCCACGGCGCTGCTGTTGGGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                             <  1:1541526/100‑1 (MQ=255)

                                   tGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTa                                                             <  1:561125/100‑1 (MQ=255)

                                       cACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAatat                                                         >  1:22214/1‑100 (MQ=255)

                                         cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGt                                                       >  2:1475039/1‑100 (MQ=255)

                                           gcgcTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTc                                                     <  1:2279085/100‑1 (MQ=255)

                                              ctgctgTTGAGCGTGGTGGCTTCTCTGTGTTCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCGCTGGCCTGTTATATGTTCggc                                                  >  1:2190684/1‑99 (MQ=255)

                                                gctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCAGCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTAt                                                >  1:4823272/1‑100 (MQ=255)

                                                  tgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGCTCGGTAttt                                              <  1:5431037/100‑1 (MQ=255)

                                                   gTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTc                                             <  1:2438241/100‑1 (MQ=255)

                                                   gTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTc                                             <  1:2005263/100‑1 (MQ=255)

                                                   gTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTc                                             <  2:488403/100‑1 (MQ=255)

                                                       aGCGTGGTGGCTGCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGCCACCACTGGCCTGTAATATGTTCGGTATTTCGaag                                         <  2:2390508/100‑2 (MQ=255)

                                                        gCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACg                                        >  2:1975311/1‑100 (MQ=255)

                                                          gtggtgGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGat                                      >  1:744229/1‑100 (MQ=255)

                                                              tggCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGGCTGTAATATGTTCGGTATTTCTAACGATATCg                                  >  1:4726475/1‑100 (MQ=255)

                                                              tggCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCg                                  >  2:1792890/1‑100 (MQ=255)

                                                               ggCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGc                                 >  1:4570347/1‑100 (MQ=255)

                                                               ggCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GGGGC‑‑GGAGGG‑GGTCACTGCTGCTGATCCCACTGGCCTGTAATATGTTCCGTATTTCGAACGATATCGc                                 >  1:4953732/1‑100 (MQ=255)

                                                                 cTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTTTTTCGAACGATATTGCCa                               >  1:3554746/1‑100 (MQ=255)

                                                                 cTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                               <  2:1314867/100‑1 (MQ=255)

                                                                 cTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                               >  2:1469716/1‑100 (MQ=255)

                                                                 cTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGGCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCa                               >  2:3172824/1‑100 (MQ=255)

                                                                     tctGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCa                           <  1:4538260/100‑1 (MQ=255)

                                                                      ctGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                          >  2:3998003/1‑100 (MQ=255)

                                                                      ctGTGTGCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAg                          >  2:2002787/1‑100 (MQ=255)

                                                                       tgtgtgCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAgg                         <  2:423194/100‑1 (MQ=255)

                                                                        gtgtgCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggt                        >  1:5155356/1‑100 (MQ=255)

                                                                        ctgtgCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTATGCTGTTGGTCCCACTGGCCTGTAATATGTTCGGTAGTTGGAACGATATCGCCATGCGggt                        >  2:2787677/2‑100 (MQ=255)

                                                                         tgtgCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtg                       <  1:2866808/100‑1 (MQ=255)

                                                                          gtgCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtgg                      >  1:1247446/1‑100 (MQ=255)

                                                                           tgCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtggt                     <  1:4967713/100‑1 (MQ=255)

                                                                           tgCCTGTGGCGCATCCGGC‑GTGGC‑‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGtt                    >  2:3354429/1‑100 (MQ=255)

                                                                           ggCCTGTGGCGCATCCGGCGGGGGG‑‑GG‑GGG‑GGTCTCTGCTGCTGATCCCATTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAggtggt                     <  2:825372/99‑1 (MQ=255)

                                                                            gCCTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGtt                    >  1:1560177/1‑100 (MQ=255)

                                                                             ccTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTg                   <  1:2068675/100‑1 (MQ=255)

                                                                              cTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTTGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGc                  >  1:831920/1‑100 (MQ=255)

                                                                              cTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCCTGCAGGTGGTTGc                  >  2:1186054/1‑100 (MQ=255)

                                                                              cTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGc                  >  2:1183657/1‑100 (MQ=255)

                                                                              cTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGc                  >  1:2493690/1‑100 (MQ=255)

                                                                              cTGTGGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGc                  >  2:3495524/1‑100 (MQ=255)

                                                                               tgtgGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGcc                 <  1:169843/100‑1 (MQ=255)

                                                                               tgtgGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGcc                 >  1:2684633/1‑100 (MQ=255)

                                                                               tgtgGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATACGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGcc                 >  1:4918121/1‑100 (MQ=255)

                                                                                gtgGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                <  2:3302135/100‑1 (MQ=255)

                                                                                gtgGCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCg                >  2:4875562/1‑100 (MQ=255)

                                                                                  ggCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATCTATTCGGTATTTCGAACGATCTCGCCATGCAGGCCGTTGCCGTc              >  1:4853421/1‑100 (MQ=255)

                                                                                  ggCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTc              >  1:3457186/1‑100 (MQ=255)

                                                                                  ggCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTc              >  2:2279626/1‑100 (MQ=255)

                                                                                  ggCGCATCCGGC‑GTGGCG‑GG‑GGG‑GGTCGCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTc              <  1:1494074/100‑1 (MQ=255)

                                                                                   gcgcATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg             <  2:655170/100‑1 (MQ=255)

                                                                                   gcgcATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg             >  2:4297449/1‑100 (MQ=255)

                                                                                    cgcATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCgg            <  1:1142262/100‑1 (MQ=255)

                                                                                    cgcATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCgg            <  1:3712088/100‑1 (MQ=255)

                                                                                    cgcATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCgg            <  1:4378822/100‑1 (MQ=255)

                                                                                     gcATCCGGG‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGc           <  2:2020442/100‑1 (MQ=255)

                                                                                     gcATCCGGC‑GGGTCG‑GG‑GGG‑GGCCTCTGGTGCTGAACCCACTGGCCGGTAATATGTTCGGTATTTCCAACGATGGCGCCGGGGAGGTGGTTGCCATCGGc           >  2:3642060/1‑100 (MQ=255)

                                                                                      cATCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt          >  2:420062/1‑100 (MQ=255)

                                                                                       aTCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCtt         <  1:1476288/100‑1 (MQ=255)

                                                                                        tCCGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCttt        >  2:2939077/1‑100 (MQ=255)

                                                                                         ccGGC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTa       >  1:3651039/1‑100 (MQ=255)

                                                                                          cgtc‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTTCCGTCGGCTTTat      >  2:2493203/4‑100 (MQ=255)

                                                                                           ggC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatc     >  1:3950094/1‑100 (MQ=255)

                                                                                           ggC‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatc     <  2:58034/100‑1 (MQ=255)

                                                                                             c‑GTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcat   <  2:442031/100‑1 (MQ=255)

                                                                                               gTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCCTGCAGGTGGTTGCCGTCGGCCTTatcctc  >  2:3061374/1‑100 (MQ=255)

                                                                                               gTGGCG‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatcatc  <  2:5084504/100‑1 (MQ=255)

GGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGC‑GTGGC‑‑GG‑GGG‑GGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATC  >  NC_000913/3240879‑3241071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A