Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F422 I52 R1	239	220.4	11079197	93.2%	10325811	100.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,276,692	A→C	N471H (AAT→CAT)	garD →	D‑galactarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,276,692	0	A	C	99.1%	744.5 / ‑4.3	210	N471H (AAT→CAT)	garD	D‑galactarate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (110/98); total (112/98)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 210 total aligned reads displayed.

CGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACC  >  NC_000913/3276595‑3276790

cGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCAt                                                                                                  <  2:4773601/100‑1 (MQ=255)

cGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCAt                                                                                                  >  2:2744933/1‑100 (MQ=255)

cGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCAt                                                                                                  >  2:1608896/1‑100 (MQ=255)

 ggTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATg                                                                                                 <  2:2119152/100‑1 (MQ=255)

   tCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCg                                                                                               <  2:4842004/100‑1 (MQ=255)

    cGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCgg                                                                                              >  1:5533857/1‑100 (MQ=255)

     gTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCggg                                                                                             >  2:3322052/1‑100 (MQ=255)

     gTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCggg                                                                                             >  2:2650372/1‑100 (MQ=255)

     gTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCggg                                                                                             <  2:2053809/100‑1 (MQ=255)

      tGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGc                                                                                            <  1:61623/100‑1 (MQ=255)

       ggTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCa                                                                                           >  1:1062453/1‑100 (MQ=255)

       ggTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCa                                                                                           >  1:3823203/1‑100 (MQ=255)

       ggTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCa                                                                                           >  1:2352862/1‑100 (MQ=255)

        gTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCAc                                                                                          >  1:2130692/1‑100 (MQ=255)

        gTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCAc                                                                                          <  2:4729765/100‑1 (MQ=255)

        gTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCAc                                                                                          >  1:3639031/1‑100 (MQ=255)

         tACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCAcc                                                                                         >  2:3621272/1‑100 (MQ=255)

           cGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCAt                                                                                       <  1:950375/100‑1 (MQ=255)

               gTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCt                                                                                   >  1:1952692/1‑100 (MQ=255)

                  cGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTAcc                                                                                <  1:3214749/100‑1 (MQ=255)

                  cGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTAcc                                                                                <  1:5026243/100‑1 (MQ=255)

                      cTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCg                                                                            >  2:2148279/1‑100 (MQ=255)

                        gATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCgaa                                                                          >  2:3299808/1‑100 (MQ=255)

                        gATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCgaa                                                                          >  1:2504886/1‑100 (MQ=255)

                        gATGGCGCTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTCGTTTGATTTAATGGATATTCATGCGGGCACCATCCCTACCGGCgaa                                                                          <  2:5104647/100‑1 (MQ=255)

                         aTGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGACCCGCTGGTTTGATTTAATCGATATTCATGCGGGCACCATCGCTACCGGCgaag                                                                         <  1:5165581/100‑1 (MQ=255)

                         aTGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCgaag                                                                         <  1:5408025/100‑1 (MQ=255)

                         aTGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCgaag                                                                         <  1:5339152/100‑1 (MQ=255)

                          tGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCgaaga                                                                        >  2:3194671/1‑100 (MQ=255)

                          tGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCgaaga                                                                        <  1:2308560/100‑1 (MQ=255)

                          tGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCgaaga                                                                        >  1:753281/1‑100 (MQ=255)

                             cGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAAc                                                                     <  2:2448979/100‑1 (MQ=255)

                             cGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAAc                                                                     <  1:5115031/100‑1 (MQ=255)

                              ggTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACt                                                                    <  2:5461806/100‑1 (MQ=255)

                               gTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCCCCATCGCTACCGGCGAAGAAACTa                                                                   >  2:280070/1‑100 (MQ=255)

                               gTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATACGGGCACCATCGCTACCGGCGAAGAAACTa                                                                   >  2:4475849/1‑100 (MQ=255)

                                 aCCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTAtt                                                                 <  2:3901461/100‑1 (MQ=255)

                                  cccGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTg                                                                >  1:1007438/1‑100 (MQ=255)

                                   ccGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGa                                                               >  1:976870/1‑100 (MQ=255)

                                    cGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGaa                                                              >  1:4305311/1‑100 (MQ=255)

                                     gTCATTAAAATGGCAACCCGCACCGAGCTTGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAag                                                             <  1:3146630/100‑1 (MQ=255)

                                        aTTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAgg                                                          <  1:2255501/100‑1 (MQ=255)

                                        aTTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAgg                                                          >  2:1933971/1‑100 (MQ=255)

                                         ttAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGt                                                         >  2:408389/1‑100 (MQ=255)

                                         ttAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGt                                                         >  1:1318209/1‑100 (MQ=255)

                                          tAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTg                                                        <  2:3425926/100‑1 (MQ=255)

                                            aaaTGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTggg                                                      <  2:1069992/100‑1 (MQ=255)

                                            aaaTGGCAACCCGCACCGAGCTAGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGTCGCAGAAACTATTGAAGAGGTggg                                                      >  2:1735130/1‑100 (MQ=255)

                                             aaTGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGc                                                     <  1:1839219/100‑1 (MQ=255)

                                              aTGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGcn                                                    <  1:4444775/100‑2 (MQ=255)

                                               tGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGGAACTATTGAGGAGGTGGGcag                                                   >  1:2411894/1‑98 (MQ=255)

                                               tGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTg                                                   >  1:2218957/1‑100 (MQ=255)

                                               tGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTg                                                   <  2:1183581/100‑1 (MQ=255)

                                                ggCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTgg                                                  <  1:4081854/100‑1 (MQ=255)

                                                  cAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGaa                                                <  2:1752415/100‑1 (MQ=255)

                                                  cAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGaa                                                >  1:3771408/1‑100 (MQ=255)

                                                   aaCCCGCACCGGGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCAGGCGGGCTCCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAg                                               <  2:3469471/100‑1 (MQ=255)

                                                   aaCCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAg                                               <  1:1674338/100‑1 (MQ=255)

                                                   aaCCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAg                                               <  1:5549720/100‑1 (MQ=255)

                                                    aCCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAgt                                              <  2:1159596/100‑1 (MQ=255)

                                                     cccGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAgtt                                             >  1:3846178/1‑100 (MQ=255)

                                                     cccGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAgtt                                             >  1:3578156/1‑100 (MQ=255)

                                                     cccGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAgtt                                             >  1:2660088/1‑100 (MQ=255)

                                                       cGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAgttgt                                           <  2:5307226/100‑1 (MQ=255)

                                                        gCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAgttgtt                                          >  1:3911597/1‑100 (MQ=255)

                                                          aCCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTcc                                        <  1:2730367/100‑1 (MQ=255)

                                                          aCCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTcc                                        >  2:3263563/1‑100 (MQ=255)

                                                           ccGATCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCa                                       <  2:381374/100‑1 (MQ=255)

                                                             gAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACt                                     <  2:670533/100‑1 (MQ=255)

                                                              aGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACtt                                    <  2:4373605/100‑1 (MQ=255)

                                                              aGCTGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACtt                                    <  1:4123293/100‑1 (MQ=255)

                                                                 tGGCGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTAt                                 <  2:1816762/100‑1 (MQ=255)

                                                                    cGAACCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATtct                              <  1:1485561/100‑1 (MQ=255)

                                                                      aaCCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCg                            <  1:2383417/100‑1 (MQ=255)

                                                                       aCCGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGTTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGa                           >  1:5014329/1‑100 (MQ=255)

                                                                        ccGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGAc                          <  2:5445194/100‑1 (MQ=255)

                                                                        ccGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGAc                          >  2:2337965/1‑100 (MQ=255)

                                                                        ccGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGAc                          >  2:1199635/1‑100 (MQ=255)

                                                                        atgCTGTTTTGATTTAATGGATATTCATTCGGGCACCATCGCTACCGGCTAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGAc                          <  2:4011373/98‑1 (MQ=255)

                                                                         cGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACg                         <  2:5237117/100‑1 (MQ=255)

                                                                         cGCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACg                         >  1:678470/1‑100 (MQ=255)

                                                                          gCTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGt                        >  2:4271439/1‑100 (MQ=255)

                                                                           cTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTc                       >  1:4713729/1‑100 (MQ=255)

                                                                           cTGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTc                       <  2:4190747/100‑1 (MQ=255)

                                                                            tGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCg                      <  2:2317947/100‑1 (MQ=255)

                                                                            tGGTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCg                      <  2:3094016/100‑1 (MQ=255)

                                                                              gTTTGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGcc                    >  1:829364/1‑100 (MQ=255)

                                                                                ttGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAg                  >  1:5439358/1‑100 (MQ=255)

                                                                                ttGATTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAg                  >  2:631075/1‑100 (MQ=255)

                                                                                   aTTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGGTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCgg               >  1:5324183/1‑100 (MQ=255)

                                                                                   aTTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCgg               <  1:1237551/100‑1 (MQ=255)

                                                                                   aTTTAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCgg               >  1:2950312/1‑100 (MQ=255)

                                                                                    tttAATGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCggg              <  2:2839957/100‑1 (MQ=255)

                                                                                       aaTGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGgaag           <  2:4072650/100‑1 (MQ=255)

                                                                                       aaTGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGgaag           <  1:1926170/100‑1 (MQ=255)

                                                                                         tGGATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGgaagaa         <  2:388178/100‑1 (MQ=255)

                                                                                          ggATATTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGCCGTCGCCAGCGGgcagaag        >  1:2463287/1‑100 (MQ=255)

                                                                                            atatTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGGGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGgaagaagaa      <  1:2915606/100‑1 (MQ=255)

                                                                                            atatTCATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGCCTGCAAGTTGTTCCACTTTATTCTCGACGTCTCTAGCGGgaagaagaa      <  1:4892535/100‑1 (MQ=255)

                                                                                                tAATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAAcc  >  2:2491162/1‑100 (MQ=255)

CGGTCGTGGTACGCCGTACGGCCTGATGGCGGTACCCGTCATTAAAATGGCAACCCGCACCGAGCTGGCGAACCGCTGGTTTGATTTAATGGATATTAATGCGGGCACCATCGCTACCGGCGAAGAAACTATTGAAGAGGTGGGCTGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACC  >  NC_000913/3276595‑3276790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A