Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F412 I2 R1 70 105.0 5244496 94.3% 4945559 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,279,878 A→G D322G (GAC→GGC)  kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,279,8780AG98.3% 408.3 / ‑3.2 120D322G (GAC→GGC) kbaZtagatose 6‑phosphate aldolase 1, kbaZ subunit
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (67/50);  total (69/51)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 120 total aligned reads displayed.

CCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGT  >  NC_000913/3279782‑3279974
                                                                                                |                                                                                                
ccTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCGGTTCTGGTCTGCGTCAGGTGCTGGAAGACGt                                                                                               >  1:486461/1‑100 (MQ=255)
 cTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTg                                                                                              >  1:1106140/1‑100 (MQ=255)
   tCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGAt                                                                                            <  2:2227336/100‑1 (MQ=255)
   tCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGAt                                                                                            >  1:1305761/1‑100 (MQ=255)
     gCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGc                                                                                          >  1:548521/1‑100 (MQ=255)
     gCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGc                                                                                          >  2:1983749/1‑100 (MQ=255)
      cccTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCt                                                                                         <  1:1800092/100‑1 (MQ=255)
      cccTGCGTGAAGCTCGGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTCGAAGGCGTGATGCt                                                                                         <  2:1871698/100‑1 (MQ=255)
        cTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCg                                                                                       <  2:2406811/100‑1 (MQ=255)
         tGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGGGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGATGGAAGGCGTGATGCTCGa                                                                                      <  1:2121093/100‑1 (MQ=255)
         cgCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCGAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGa                                                                                      <  2:17151/99‑1 (MQ=255)
          gCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGAc                                                                                     <  1:289447/100‑1 (MQ=255)
          gCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGAc                                                                                     <  2:176783/100‑1 (MQ=255)
              gAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGcc                                                                                 <  1:457166/100‑1 (MQ=255)
                 gCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCgg                                                                              >  2:1947958/1‑100 (MQ=255)
                 gCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCgg                                                                              <  2:1099476/100‑1 (MQ=255)
                 gCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATACTCGACCGCCCgg                                                                              >  2:2036139/1‑100 (MQ=255)
                  ctctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGa                                                                             >  2:1113010/1‑100 (MQ=255)
                   tctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGaa                                                                            >  2:2145778/1‑100 (MQ=255)
                   tctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGATCTGGTCTGCGTCGGGTGCTGGAAGGCGTGATGCTTGACCGCCCGGaa                                                                            >  1:2514938/1‑100 (MQ=255)
                    ctGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAAt                                                                           <  2:1807722/100‑1 (MQ=255)
                       ttCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACt                                                                        >  1:144308/1‑100 (MQ=255)
                         ctctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTgg                                                                      <  1:148431/100‑1 (MQ=255)
                         ctctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTgg                                                                      >  1:2242586/1‑100 (MQ=255)
                         ctctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTgg                                                                      <  1:2229711/100‑1 (MQ=255)
                          tctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGc                                                                     <  1:1263740/100‑1 (MQ=255)
                          tctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGc                                                                     <  1:5472/100‑1 (MQ=255)
                          tctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGc                                                                     <  1:54994/100‑1 (MQ=255)
                           ctctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCa                                                                    >  2:2005399/1‑100 (MQ=255)
                             ctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCaaa                                                                  >  1:1680632/1‑100 (MQ=255)
                               ggcggcGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGc                                                                >  2:2548434/1‑100 (MQ=255)
                               ggcggcGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGc                                                                >  1:762938/1‑100 (MQ=255)
                                gcggcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGcc                                                               <  1:2533206/100‑1 (MQ=255)
                                   gcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACt                                                            <  1:2535361/100‑1 (MQ=255)
                                    cgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTa                                                           >  2:1914591/1‑100 (MQ=255)
                                    cgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTa                                                           <  2:1649598/100‑1 (MQ=255)
                                    cgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTa                                                           >  2:945458/1‑100 (MQ=255)
                                    cgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTa                                                           >  1:2175690/1‑100 (MQ=255)
                                     gATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTAc                                                          >  1:1369660/1‑100 (MQ=255)
                                      aTTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTAcc                                                         >  1:387048/1‑100 (MQ=255)
                                      aTTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTAcc                                                         >  1:426846/1‑100 (MQ=255)
                                        tGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCAc                                                       >  1:2040163/1‑100 (MQ=255)
                                           agaagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGt                                                    >  1:1099879/1‑100 (MQ=255)
                                            gaagaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTg                                                   >  2:555303/1‑100 (MQ=255)
                                              agaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGAc                                                 >  2:761973/1‑100 (MQ=255)
                                              agaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGAc                                                 <  1:2497094/100‑1 (MQ=255)
                                               gaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCGCTACCACGGTGACg                                                <  2:1974239/100‑1 (MQ=255)
                                               gaaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACg                                                <  2:867068/100‑1 (MQ=255)
                                                aaCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACgg                                               <  2:1257385/100‑1 (MQ=255)
                                                 aCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGc                                              <  1:2319629/100‑1 (MQ=255)
                                                 aCTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGc                                              >  1:1179652/1‑100 (MQ=255)
                                                    ggTGCCAGCGAAAGCCTGCTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAc                                           >  1:1946364/1‑100 (MQ=255)
                                                     gTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcg                                          >  2:620990/1‑100 (MQ=255)
                                                      tGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgc                                         >  2:143944/1‑100 (MQ=255)
                                                       gCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcg                                        >  1:931549/1‑100 (MQ=255)
                                                       gCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcg                                        <  2:937714/100‑1 (MQ=255)
                                                         cAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcgcg                                      >  2:2461678/1‑100 (MQ=255)
                                                         cAGCGAAAGCCTGTTCTGGTCTGCGGCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcgcg                                      <  2:1693799/100‑1 (MQ=255)
                                                             gAAAGCCTGTTATGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGAAAAGCCACTACCACGGTGACGGCAACGCGcgtcgt                                  >  2:2536404/1‑100 (MQ=255)
                                                                aGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGTCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTATCACGGTGACTGCATCGTGCGTCGTCTg                               <  2:1184503/100‑1 (MQ=255)
                                                                aGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTg                               <  1:6742/100‑1 (MQ=255)
                                                                 gCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTgg                              >  2:2213995/1‑100 (MQ=255)
                                                                   cTGTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcg                            <  2:1398360/100‑1 (MQ=255)
                                                                     gTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGcgcg                          >  2:799364/1‑100 (MQ=255)
                                                                     gTTCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGcgcg                          >  1:985797/1‑100 (MQ=255)
                                                                      ttCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGt                         <  1:1070826/100‑1 (MQ=255)
                                                                       tCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTg                        <  1:155051/100‑1 (MQ=255)
                                                                       tCTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTg                        <  1:1836014/100‑1 (MQ=255)
                                                                        cTGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTgg                       >  2:2296402/1‑100 (MQ=255)
                                                                         tGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGt                      <  1:1929239/100‑1 (MQ=255)
                                                                         tGGTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCACGTCGTCTGGCGCGTGGt                      <  1:1658056/100‑1 (MQ=255)
                                                                          ggTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGGCCGGAATACTGGCAAAGCCACTACCACGGAGACAGAACGGCGCGTCGTCTGGCGCGTGGtt                     >  2:1397978/1‑100 (MQ=255)
                                                                          ggTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGtt                     >  1:1590109/1‑100 (MQ=255)
                                                                          ggTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGtt                     <  2:2276042/100‑1 (MQ=255)
                                                                           gTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTta                    >  2:1557622/1‑100 (MQ=255)
                                                                           gTCTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTta                    <  2:2002192/100‑1 (MQ=255)
                                                                             cTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTtata                  <  1:925855/100‑1 (MQ=255)
                                                                             cTGCGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTtata                  <  1:792214/100‑1 (MQ=255)
                                                                                cGTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGcn               <  1:2094298/100‑2 (MQ=255)
                                                                                 gTCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTa              >  1:1046214/1‑100 (MQ=255)
                                                                                  tCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTAc             >  2:582649/1‑100 (MQ=255)
                                                                                  tCAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTAc             >  2:438270/1‑100 (MQ=255)
                                                                                   cAGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACt            <  1:1778629/100‑1 (MQ=255)
                                                                                    aGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTc           >  2:1400701/1‑100 (MQ=255)
                                                                                    aGGTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTc           >  2:1142251/1‑100 (MQ=255)
                                                                                     ggTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCg          >  1:249007/1‑100 (MQ=255)
                                                                                     ggTGCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACAGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCg          >  1:80031/1‑100 (MQ=255)
                                                                                        gCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGAt       >  2:1430229/1‑100 (MQ=255)
                                                                                        gCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGAt       >  1:918138/1‑100 (MQ=255)
                                                                                        gCTGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGAt       <  1:1993828/100‑1 (MQ=255)
                                                                                          tGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcg     >  1:639794/1‑100 (MQ=255)
                                                                                          tGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcg     >  1:997944/1‑100 (MQ=255)
                                                                                          tGGAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcg     >  1:1292160/1‑100 (MQ=255)
                                                                                          tGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcg     >  1:2355414/1‑100 (MQ=255)
                                                                                           ggAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgc    >  1:42664/1‑100 (MQ=255)
                                                                                           ggAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgc    >  2:821543/1‑100 (MQ=255)
                                                                                           ggAAGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgc    >  1:379091/1‑100 (MQ=255)
                                                                                           ggAAGGCGTGATCCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgc    >  1:2272842/1‑100 (MQ=255)
                                                                                             aaGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGt  >  2:2422738/1‑100 (MQ=255)
                                                                                             aaGGCGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGAGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGt  >  2:2355421/1‑100 (MQ=255)
                                                                                                |                                                                                                
CCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGT  >  NC_000913/3279782‑3279974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A