Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F422 I52 R1 239 220.4 11079197 93.2% 10325811 100.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,414,497 T→G Y212D (TAC→GAC)  acrE → cytoplasmic membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,414,4970TG99.1% 793.0 / ‑4.4 221Y212D (TAC→GAC) acrEcytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base G (109/109);  total (112/109)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 221 total aligned reads displayed.

TAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAG  >  NC_000913/3414400‑3414593
                                                                                                 |                                                                                                
tAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGAc                                                                                                <  1:4387069/100‑1 (MQ=255)
 aGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACg                                                                                               >  2:4586797/1‑100 (MQ=255)
 aGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATCGGCCAACGACTGAACTGGCGACTGTCCCGCAGCTCGAGCCTGTCGACg                                                                                               >  2:3161875/1‑100 (MQ=255)
  gCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGt                                                                                              >  1:2727600/1‑100 (MQ=255)
   cGGACGTATCGGCAAATCGACTGTGACCGAAGGCTCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGtt                                                                                             <  2:294502/100‑1 (MQ=255)
    ggACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTg                                                                                            <  2:2056432/100‑1 (MQ=255)
      aCGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAt                                                                                          <  2:3495371/100‑1 (MQ=255)
      aCGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAt                                                                                          >  1:2605462/1‑100 (MQ=255)
       cGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtg                                                                                         <  1:590915/100‑1 (MQ=255)
       cGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtg                                                                                         <  1:3877528/100‑1 (MQ=255)
       cGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtg                                                                                         <  1:2189381/100‑1 (MQ=255)
       cGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtg                                                                                         <  1:1328785/100‑1 (MQ=255)
        gTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtgt                                                                                        >  1:796378/1‑100 (MQ=255)
        gTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtgt                                                                                        >  1:5479753/1‑100 (MQ=255)
        gTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtgt                                                                                        >  1:950526/1‑100 (MQ=255)
        gTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtgt                                                                                        >  2:5443200/1‑100 (MQ=255)
         tATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtgtg                                                                                       <  2:1660002/100‑1 (MQ=255)
         tATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtgtg                                                                                       >  2:4439358/1‑100 (MQ=255)
          aTCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGa                                                                                      >  2:3972560/1‑100 (MQ=255)
           tCGGCAAATCGACTCTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGAc                                                                                     <  2:5019190/100‑1 (MQ=255)
            cGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGAtgtagat                                                                                    >  2:4057777/1‑96 (MQ=255)
            cGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGAcc                                                                                    >  2:1838224/1‑100 (MQ=255)
             ggCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGAccc                                                                                   >  1:4263838/1‑100 (MQ=255)
             ggCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGAccc                                                                                   >  2:1317395/1‑100 (MQ=255)
             ggCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGAccc                                                                                   >  1:5102443/1‑100 (MQ=255)
                aaaTCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAAt                                                                                >  1:2152270/1‑100 (MQ=255)
                  agcGACTGTGACCGAAGGCGCTCTTGTCACTACTGGGCAAACGACTGAACTGGCGACTGTCCAGCCGCTCGATCCTGTCGACGTTGATGTGACCCAATcc                                                                              <  2:3594299/98‑1 (MQ=255)
                  aTCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATcc                                                                              >  1:2785293/1‑100 (MQ=255)
                  aTCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATcc                                                                              <  1:4615547/100‑1 (MQ=255)
                  aTCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATcc                                                                              >  1:4022668/1‑100 (MQ=255)
                     gACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAGACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGc                                                                           <  1:5251951/100‑1 (MQ=255)
                     gACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGc                                                                           <  1:4240124/100‑1 (MQ=255)
                     gACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGc                                                                           <  1:3049567/100‑1 (MQ=255)
                     gACTGTGACCGAAGGCGCTCTTGACACTAAGGGGCAAACGACTGAACTGGCGACCGTCCAGCAGCTCGATCATATCGACGTTGATGTGACCCAAGCCAGc                                                                           <  2:3369822/100‑1 (MQ=255)
                       cTGTGACCGAAGGTGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCaa                                                                         <  2:3729198/100‑1 (MQ=255)
                       cTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCaa                                                                         >  1:3783378/1‑100 (MQ=255)
                       cTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCaa                                                                         <  2:1468323/100‑1 (MQ=255)
                       cTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCaa                                                                         >  1:3901499/1‑100 (MQ=255)
                         gtgACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACg                                                                       <  1:5296362/100‑1 (MQ=255)
                         gtgACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACg                                                                       >  2:3644243/1‑100 (MQ=255)
                           naCCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGAt                                                                     >  1:4807647/2‑100 (MQ=255)
                             ccGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGAttt                                                                   >  1:4408896/1‑100 (MQ=255)
                               gAAGGCGCTCTTGTCGCTAAAGCGCAAACGACTAAACTGGCTACCCTCCAGCAGCTCGATCCTATCGACGTTGATGTGTCCCAATCCAGCAACTATTTTa                                                                 <  2:5235788/100‑1 (MQ=255)
                               gAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTa                                                                 >  2:2044451/1‑100 (MQ=255)
                               gAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTa                                                                 >  1:2541602/1‑100 (MQ=255)
                               gAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTa                                                                 >  2:1504106/1‑100 (MQ=255)
                                 aGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATg                                                               <  2:3094788/100‑1 (MQ=255)
                                    cgcTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAgg                                                            >  1:4962447/1‑100 (MQ=255)
                                      ctctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCt                                                          <  2:4367568/100‑1 (MQ=255)
                                      ctctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCt                                                          <  1:923755/100‑1 (MQ=255)
                                        ctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGa                                                        >  2:2814057/1‑100 (MQ=255)
                                        ctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGa                                                        <  2:2155774/100‑1 (MQ=255)
                                        ctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGa                                                        >  1:1809093/1‑100 (MQ=255)
                                           ttCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGc                                                     <  1:3473374/99‑1 (MQ=255)
                                            tCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCa                                                    >  2:3441114/1‑100 (MQ=255)
                                             cACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCaa                                                   >  2:3663485/1‑100 (MQ=255)
                                               cTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATc                                                 >  1:4477904/1‑100 (MQ=255)
                                               cTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATc                                                 >  1:4569400/1‑100 (MQ=255)
                                               cTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATc                                                 >  1:414963/1‑100 (MQ=255)
                                                    gggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTag                                            >  1:2224537/1‑100 (MQ=255)
                                                     ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTaga                                           <  1:1753224/100‑1 (MQ=255)
                                                     ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTaga                                           <  1:3125034/100‑1 (MQ=255)
                                                      gCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGCTGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTagcg                                          >  2:2274569/1‑98 (MQ=255)
                                                       cAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGc                                         >  2:3614557/1‑100 (MQ=255)
                                                       cAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGc                                         <  2:1193367/100‑1 (MQ=255)
                                                        aaaCGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCa                                        >  1:163419/1‑100 (MQ=255)
                                                        aaaCGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCa                                        >  1:1514250/1‑100 (MQ=255)
                                                         aaCGACTGAACTGGCGACTGTCCAGCAGCTCGATCTTGTCCACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGCAGCAATCCCTAGAGCaa                                       >  2:5361290/1‑100 (MQ=38)
                                                             aCTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGaa                                   >  2:4331992/1‑100 (MQ=255)
                                                                gAACTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAAtt                                <  1:1859500/100‑1 (MQ=255)
                                                                  aCTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTg                              <  2:3995993/100‑1 (MQ=255)
                                                                  aCTGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTg                              <  1:3639056/100‑1 (MQ=255)
                                                                    tGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCa                            <  1:1019425/100‑1 (MQ=255)
                                                                    tGGCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTCTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCa                            <  2:4224868/100‑1 (MQ=255)
                                                                    tGGCGACTGTCCAGCAGCTCGATCCGATCGACGTTAATGAGACCCAATCCAGCAACCATTTTATGGGGCTGAAGCAATCCGTAGAGCAAGGAAATGTGCa                            <  2:4658399/100‑1 (MQ=255)
                                                                     ggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGCTTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCAt                           >  1:1412053/1‑100 (MQ=255)
                                                                     ggCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCAt                           <  1:5141790/100‑1 (MQ=255)
                                                                     ggCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCAt                           <  1:3734535/100‑1 (MQ=255)
                                                                     ggCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCAt                           >  1:3706933/1‑100 (MQ=255)
                                                                     ggCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCAt                           <  1:2323908/100‑1 (MQ=255)
                                                                      gCGACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATa                          <  1:863684/100‑1 (MQ=255)
                                                                        gACTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCCGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAg                        >  2:388883/1‑100 (MQ=255)
                                                                        gACTGTCCAGCAGCACGAGCCTATGGACGTTGATGGGACCTAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAg                        <  2:4509817/100‑1 (MQ=255)
                                                                         aCTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAgg                       <  2:2587194/100‑1 (MQ=255)
                                                                          cTGTCCAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGTCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGa                      >  2:556809/1‑100 (MQ=255)
                                                                              ccAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAAc                  >  1:3623050/1‑100 (MQ=255)
                                                                              ccAGCAGCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAAc                  <  1:1346448/100‑1 (MQ=255)
                                                                               cagcagCTCGATCCTATCGACGTTGATGTGTCCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACg                 <  2:4836602/100‑1 (MQ=255)
                                                                               cagcagCTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACg                 <  2:699907/100‑1 (MQ=255)
                                                                                agcagcTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGc                >  1:1186526/1‑100 (MQ=255)
                                                                                  cagcTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGcca              <  1:5417616/100‑1 (MQ=255)
                                                                                   agcTCGATCCTATCGACCTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGCAAATTTGCATAAGGAAAACGccac             <  1:2918443/100‑1 (MQ=255)
                                                                                    gcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGccacc            >  2:324083/1‑100 (MQ=255)
                                                                                    gcTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGccacc            <  2:5123119/100‑1 (MQ=255)
                                                                                    gcTCGATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGccacc            >  1:4880039/1‑100 (MQ=255)
                                                                                      tCGATCCTATCGACGTTGATGTGACCCAAACCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAg          <  1:4924913/100‑1 (MQ=255)
                                                                                        gATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAATTTTGCATAAGGAAAACGCCACCAGCa        >  1:4528447/1‑100 (MQ=255)
                                                                                        gATCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCa        >  2:4999955/1‑100 (MQ=255)
                                                                                         aTCCTATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCaa       >  1:892356/1‑100 (MQ=255)
                                                                                             tATCGACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTa   >  2:1812412/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TAGCGGACGTATCGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAG  >  NC_000913/3414400‑3414593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A